Difference between revisions of "Template:IONIS Paris/Notebook/09-09-15"
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<i class="fa fa-caret-right"></i> Typical profile of colonies transformed with pDawn-HOKD under light exposition<br><br> | <i class="fa fa-caret-right"></i> Typical profile of colonies transformed with pDawn-HOKD under light exposition<br><br> | ||
| − | <center><img src="https://static.igem.org/mediawiki/2015/a/af/Res0909.png" alt="img01" class="img-responsive" style="width: | + | <center><img src="https://static.igem.org/mediawiki/2015/a/af/Res0909.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br> |
<i class="fa fa-angle-right"></i>Continuous bacterial growth despite light exposure <i class="fa fa-caret-right"></i> not expected | <i class="fa fa-angle-right"></i>Continuous bacterial growth despite light exposure <i class="fa fa-caret-right"></i> not expected | ||
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Revision as of 09:46, 16 September 2015
E.coli DH5 alpha transformation
Transformation of competent cells with pSB1C3-GFP and pSB1C3-H/E (ratio 3:1, 1:1, 1:3) with 3 µl of plasmid.
Liquid cultures on plates from 08/09
Measurements of DO595 and DO405 (wtGFP) all the day long
Typical profile of colonies transformed with pDawn-HOKD under light exposition
Continuous bacterial growth despite light exposure not expected
9 September 15