Difference between revisions of "Team:NTNU Trondheim/Notebook"
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<html> | <html> | ||
− | + | <body> | |
− | + | <p><!-- TOUR TEMPLATE --> | |
− | + | </p> | |
− | + | ||
− | + | ||
− | + | <div class="tour" style="left:0;display:none"> | |
− | + | <a class="button radius" id="tourPrev">Previous<br> | |
− | + | Tour Page</a> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | <div class="tour" style="right:0;display:none"> | |
− | + | <a class="button radius" id="tourNext">Next<br> | |
− | + | Tour Page</a> | |
− | + | </div> | |
− | + | ||
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− | + | <div class="tour" style="right:0;display:none"> | |
− | + | <a class="button radius" id="tourStart">Start<br> | |
− | + | Tour!</a> | |
− | + | </div> | |
− | + | ||
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− | + | <div class="row"> | |
− | + | <div class="two columns"> | |
− | + | <ul class="side-nav" style="margin-left:15px"> | |
− | + | <!-- ===================Month Filters=================== --> | |
− | + | ||
− | + | ||
− | + | <li> | |
− | + | <h6>Jump to:</h6> | |
− | + | </li> | |
− | + | <!--li class="nb-month"> | |
− | + | <div style="top:67px;margin-left:-6px">Spring</div> | |
− | + | </li> | |
− | + | <li id="weekA" class="active" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#A">January</a> | |
− | + | </li> | |
− | + | <li id="weekB" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#B">February</a> | |
− | + | </li> | |
− | + | <li id="weekC" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#C">March</a> | |
− | + | </li--> | |
− | + | ||
− | + | ||
− | + | <li class="nb-month"> | |
− | + | <div style="top:7px"> | |
− | + | April | |
− | + | </div> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week18" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#18">Week 18</a> | |
− | + | </li> | |
− | + | <!--li id="weekE" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#E">May</a> | |
− | + | </li--> | |
− | + | ||
− | + | ||
− | + | <li class="nb-month"> | |
− | + | <div style="top:45px"> | |
− | + | June | |
− | + | </div> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week23" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#23">Week 23</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week24" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#24">Week 24</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week25" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#25">Week 25</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week26" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#26">Week 26</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li class="nb-month"> | |
− | + | <div style="top:65px;margin-left:2px"> | |
− | + | July | |
− | + | </div> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week27" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#27">Week 27</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week28" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#28">Week 28</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week29" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#29">Week 29</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week30" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#30">Week 30</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week31" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#31">Week 31</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li class="nb-month"> | |
− | + | <div style="top:52px;margin-left:-9px"> | |
− | + | August | |
− | + | </div> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week32" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#32">Week 32</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week33" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#33">Week 33</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week34" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#34">Week 34</a> | |
− | + | </li> | |
− | + | ||
− | + | ||
− | + | <li id="week35" onclick="weekFilter(this)"> | |
− | + | <a class="nb-week" href="#35">Week 35</a> | |
− | + | </li> | |
− | + | ||
+ | |||
+ | <li class="nb-month"> | ||
+ | <div style="top:52px;margin-left:-22px"> | ||
+ | September | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week36" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#36">Week 36</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week37" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#37">Week 37</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week38" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#38">Week 38</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week39" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#39">Week 39</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li class="nb-month"> | ||
+ | <div style="top:55px;margin-left:-12px"> | ||
+ | October | ||
+ | </div> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week40" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#40">Week 40</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week41" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#41">Week 41</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week42" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#42">Week 42</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li id="week43" onclick="weekFilter(this)"> | ||
+ | <a class="nb-week" href="#43">Week 43</a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="ten columns team-bios-container"> | ||
+ | <div class="row" id="ugd-members"> | ||
+ | <div class="twelve columns"> | ||
+ | <h2 class="centered">Notebook</h2> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- ===================Subteam Filters=================== --> | ||
+ | |||
+ | |||
+ | <div class="row last-ele" style="padding-bottom:0px"> | ||
+ | <h4 style="margin-left:15px;text-align:left">Filter by | ||
+ | subteam:</h4> | ||
+ | |||
+ | |||
+ | <div class="nb-all" id="nb-all" onclick="showAll()"> | ||
+ | <h4 style="margin-left:15px;text-align:left">show all | ||
+ | subteams</h4> | ||
+ | |||
+ | |||
+ | <div class="nb-all-i" id="nb-alltech" onclick= | ||
+ | "showAllTech()" style="margin-right:15px"> | ||
+ | <h4 style="margin-left:15px;text-align:left">show | ||
+ | technical details</h4> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="row last-ele nb-labels" style="padding-bottom:0px"> | ||
+ | <div style="opacity:0.00;width:65px"> | ||
+ | "_" | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | Wet Lab | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | Dry Lab | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | Human Practices | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | Wiki | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div> | ||
+ | Presenters | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="row"> | ||
+ | <!-- PLACEHOLDER --> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter" style= | ||
+ | "opacity:0.00;width:65px"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter"> | ||
+ | <div class="filter-container" onclick="filter(this)" style= | ||
+ | "background-image:url(https://static.igem.org/mediawiki/2015/3/3a/Team_NTNU_Trondheim_nb-wet-bg.png)"> | ||
+ | <img id="wet" src= | ||
+ | "https://static.igem.org/mediawiki/2015/c/c6/Team_NTNU_Trondheim_nb-wet-bc.png"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-only" id="wet-only" onclick= | ||
+ | "onlyFilter(this)"> | ||
+ | only | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter"> | ||
+ | <div class="filter-container" onclick="filter(this)" style= | ||
+ | "background-image:url(https://static.igem.org/mediawiki/2015/2/27/Team_NTNU_Trondheim_nb-dry-sg.png)"> | ||
+ | <img id="dry" src= | ||
+ | "https://static.igem.org/mediawiki/2015/b/b4/Team_NTNU_Trondheim_nb-dry-sc.png"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-only" id="dry-only" onclick= | ||
+ | "onlyFilter(this)"> | ||
+ | only | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter"> | ||
+ | <div class="filter-container" onclick="filter(this)" style= | ||
+ | "background-image:url(https://static.igem.org/mediawiki/2015/8/8c/Team_NTNU_Trondheim_nb-hprac-sg.png)"> | ||
+ | <img id="hprac" src= | ||
+ | "https://static.igem.org/mediawiki/2015/9/9b/Team_NTNU_Trondheim_nb-hprac-sc.png"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-only" id="hprac-only" onclick= | ||
+ | "onlyFilter(this)"> | ||
+ | only | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter"> | ||
+ | <div class="filter-container" onclick="filter(this)" style= | ||
+ | "background-image:url(https://static.igem.org/mediawiki/2015/d/d6/Team_NTNU_Trondheim_nb-wiki-sg.png)"> | ||
+ | <img id="wiki" src= | ||
+ | "https://static.igem.org/mediawiki/2015/9/98/Team_NTNU_Trondheim_nb-wiki-sc.png"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-only" id="wiki-only" onclick= | ||
+ | "onlyFilter(this)"> | ||
+ | only | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter"> | ||
+ | <div class="filter-container" onclick="filter(this)" style= | ||
+ | "background-image:url(https://static.igem.org/mediawiki/2015/a/a2/Team_NTNU_Trondheim_nb-pres-sg.png)"> | ||
+ | <img id="anim" src= | ||
+ | "https://static.igem.org/mediawiki/2015/0/0d/Team_NTNU_Trondheim_nb-pres-sc.png"> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-only" id="anim-only" onclick= | ||
+ | "onlyFilter(this)"> | ||
+ | only | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- PLACEHOLDER --> | ||
+ | |||
+ | |||
+ | <div class="two columns nb-filter" style= | ||
+ | "opacity:0.00;width:65px"> | ||
+ | </div> | ||
+ | </div> | ||
<!-- ===================Notebook entries=================== --> | <!-- ===================Notebook entries=================== --> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
− | <div class="nb-week" id=" | + | |
+ | |||
+ | <div class="nb-week" id="week18entry"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
− | <h3 class="centered">Week | + | <h3 class="centered">Week 18</h3> |
− | <h5 class="centered">( | + | |
− | + | ||
− | + | <h5 class="centered">(28/04 - 04/05)</h5> | |
− | + | ||
− | + | ||
− | + | <p> | |
− | + | </p> | |
− | + | ||
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− | + | ||
− | + | ||
− | + | ||
<div class="entry nb-hprac"> | <div class="entry nb-hprac"> | ||
<div> | <div> | ||
− | <h6> | + | <h6>April 29th</h6> |
− | + | ||
− | + | ||
− | < | + | <div class="nb-onetech-i nohilite"> |
− | + | <h6>show technical details</h6> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | <div class="nb-tech"> | |
− | < | + | {{{techdetail}}} |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | <p> | |
− | + | </p> | |
− | + | ||
− | + | ||
− | + | <div style="margin-left: 2em"> | |
− | + | Open Day at NTNU. The NTNU iGEM team explained | |
− | + | about synthetic biology and iGEM, as well as | |
− | + | showed and explained about some simple | |
− | + | experimental methods (e.g. gel | |
− | + | electrophoresis). | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | <p> | |
− | + | </p> | |
− | + | ||
− | + | ||
− | < | + | |
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− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | </ | + | |
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-week" id="week23entry" style="display: block"> | ||
+ | <div class="twelve columns"> | ||
+ | <h3 class="centered">Week 23</h3> | ||
+ | |||
+ | |||
+ | <h5 class="centered">(02/06 - 08/06)</h5> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
+ | |||
<div class="nb-week" id="week24entry"> | <div class="nb-week" id="week24entry"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
<h3 class="centered">Week 24</h3> | <h3 class="centered">Week 24</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(09/06 - 15/06)</h5> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
+ | |||
<div class="nb-week" id="week25entry"> | <div class="nb-week" id="week25entry"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
<h3 class="centered">Week 25</h3> | <h3 class="centered">Week 25</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(16/06 - 22/06)</h5> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
+ | |||
<div class="nb-week" id="week26entry"> | <div class="nb-week" id="week26entry"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
<h3 class="centered">Week 26</h3> | <h3 class="centered">Week 26</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(23/06 - 29/06)</h5> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
+ | |||
<div class="nb-week" id="week27entry"> | <div class="nb-week" id="week27entry"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
<h3 class="centered">Week 27</h3> | <h3 class="centered">Week 27</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(30/06 - 06/07)</h5> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
+ | |||
+ | |||
<div class="nb-week" id="week28entry"> | <div class="nb-week" id="week28entry"> | ||
<div class="twelve columns"> | <div class="twelve columns"> | ||
<h3 class="centered">Week 28</h3> | <h3 class="centered">Week 28</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(07/07 - 13/07)</h5> | ||
+ | |||
+ | |||
<p> | <p> | ||
</p> | </p> | ||
− | + | ||
+ | |||
+ | <div class="entry nb-wet"> | ||
+ | <div> | ||
+ | <h6>July 7th</h6> | ||
+ | |||
+ | |||
+ | <div class="nb-onetech-i nohilite"> | ||
+ | <h6>show technical details</h6> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-tech"> | ||
+ | NanoDrop of amplified backbone showed an | ||
+ | average concentration of 50 ng/mL for all | ||
+ | samples. The ligation ratio of insert and | ||
+ | backbone was 3. <i>T4</i> ligase was used. The | ||
+ | next day colonies were observed for both | ||
+ | ligations. | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <ol> | ||
+ | <li>Amplification of <a href= | ||
+ | "http://parts.igem.org/Part:pSB1A3">psB1A3 | ||
+ | backbone</a>, <a href= | ||
+ | "http://parts.igem.org/Part:pSB1C3">psB1C3 | ||
+ | backbone</a> and <a href= | ||
+ | "http://parts.igem.org/Part:pSB1K3">psB1K3 | ||
+ | backbone</a> using touchdown <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#9"> | ||
+ | PCR</a>. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li> | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#8"> | ||
+ | Digestion</a>, <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#12"> | ||
+ | ligation</a> and <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#13"> | ||
+ | heat-shock transformed</a> of <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#17"> | ||
+ | Left_flank</a>, JB with psB1A3 backbone and | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#18"> | ||
+ | Kanamycin_resistance</a>, <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#16"> | ||
+ | Righ_flank</a> with psB1A3 backbone. | ||
+ | </li> | ||
+ | </ol> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="entry nb-wet"> | ||
+ | <div> | ||
+ | <h6>July 8th</h6> | ||
+ | |||
+ | |||
+ | <div class="nb-onetech-i nohilite"> | ||
+ | <h6>show technical details</h6> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-tech"> | ||
+ | Construct was optimised to work in | ||
+ | <i>Synechocystis</i> sp. PCC 6803. | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <ol> | ||
+ | <li>Made <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#1"> | ||
+ | LB plates</a> with ampicillin and with | ||
+ | chloramphenicol. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li> | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#13"> | ||
+ | Heat-shock transformed</a> synthesised | ||
+ | promoter + RBS region (<a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#19">Construct</a>) | ||
+ | into competent <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#21"> | ||
+ | <i>E. coli</i> DH5α</a>. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li>One colony from each ligation on July 7th | ||
+ | were picked and inoculated.</li> | ||
+ | </ol> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="entry nb-wet"> | ||
+ | <div> | ||
+ | <h6>July 9th</h6> | ||
+ | |||
+ | |||
+ | <div class="nb-onetech-i nohilite"> | ||
+ | <h6>show technical details</h6> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-tech"> | ||
+ | NanoDrop of LF+JB and Kan+RF showed a | ||
+ | concentration of 44.7 and 47.6 ng/mL | ||
+ | respectively. BBa_E1010 was picked from plate | ||
+ | 3, 11N. | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <ol> | ||
+ | <li> | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#7"> | ||
+ | Mini-prep</a> of inoculated colonies of | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#17"> | ||
+ | Left_flank</a>+JB and <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#18"> | ||
+ | Kanamycin_resistance</a>+<a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#16">Righ_flank</a>. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li> | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#8"> | ||
+ | Digestion</a>, <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#12"> | ||
+ | ligation</a> and <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#13"> | ||
+ | heat-shock transformation</a> of LFJB, | ||
+ | <a href= | ||
+ | "http://parts.igem.org/Part:BBa_C0012">BBa_C0012</a> | ||
+ | and <a href= | ||
+ | "http://parts.igem.org/Part:pSB1C3">psB1C3 | ||
+ | backbone</a>. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li>Rehydrated BioBrick <a href= | ||
+ | "http://parts.igem.org/Part:BBa_E1010">BBa_E1010</a> | ||
+ | and heat-shock transformed into competent | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#21"> | ||
+ | <i>E. coli</i> DH5α</a>. | ||
+ | </li> | ||
+ | </ol> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="entry nb-wet"> | ||
+ | <div> | ||
+ | <h6>July 10th</h6> | ||
+ | |||
+ | |||
+ | <div class="nb-onetech-i nohilite"> | ||
+ | <h6>show technical details</h6> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div class="nb-tech"> | ||
+ | The sequencing results arrived approximately a | ||
+ | week later with news that the sequence that was | ||
+ | submitted did not correspond to the desired | ||
+ | sequence. The next day BBa_E1010 and KRF did | ||
+ | not show growth in the liquid medium. | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | |||
+ | |||
+ | <ol> | ||
+ | <li> | ||
+ | <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#15"> | ||
+ | Gel electrophoresis verification</a> of | ||
+ | digest of <a href= | ||
+ | "https://2015.igem.org/Team:NTNU_Trondheim/Protocols#17"> | ||
+ | Left_flank</a>+JB, <a href= | ||
+ | "http://parts.igem.org/Part:BBa_C0012">BBa_C0012</a>, | ||
+ | <a href= | ||
+ | "http://parts.igem.org/Part:BBa_E1010">BBa_E1010</a> | ||
+ | and <a href= | ||
+ | "http://parts.igem.org/Part:pSB1C3">psB1C3 | ||
+ | backbone</a> from July 9th on agarose gel | ||
+ | using <i>Not</i>I-HF restriction enzyme. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li>LFJB on <a href= | ||
+ | "http://parts.igem.org/Part:pSB1A3">psB1A3 | ||
+ | backbone</a> and KRF on psB1A3 backbone sent | ||
+ | for sequencing. | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li>Inoculated new colonies of LFJB and KRF and | ||
+ | BBa_E1010 because the gel verification did not | ||
+ | give expected bands.</li> | ||
+ | </ol> | ||
+ | |||
+ | |||
+ | <p> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
+ | |||
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+ | |||
+ | |||
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<h3 class="centered">Week 29</h3> | <h3 class="centered">Week 29</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(14/07 - 20/07)</h5> | ||
+ | |||
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+ | <h5 class="centered">(04/08 - 10/08)</h5> | ||
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+ | <h5 class="centered">(08/09 - 14/09)</h5> | ||
+ | |||
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<h3 class="centered">Week 38</h3> | <h3 class="centered">Week 38</h3> | ||
− | <h5 class="centered">( | + | |
+ | |||
+ | <h5 class="centered">(15/09 - 21/09)</h5> | ||
+ | |||
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+ | <h5 class="centered">(22/09 - 28/09)</h5> | ||
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+ | |||
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+ | |||
+ | |||
+ | <div class="nb-week" id="week40entry"> | ||
+ | <div class="twelve columns"> | ||
+ | <h3 class="centered">Week 40</h3> | ||
+ | |||
+ | |||
+ | <h5 class="centered">(29/09 - 05/10)</h5> | ||
+ | |||
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+ | <p> | ||
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+ | </div> | ||
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− | <div class="printfooter"> | + | |
− | Retrieved from "<a href="https://2013.igem.org/Team:Cornell/notebook">https://2013.igem.org/Team:Cornell/notebook</a>" and "<a href="https://2014.igem.org/Team:NTNU_Trondheim/notebook">https://2014.igem.org/Team:NTNU_Trondheim/notebook</a>"</div> | + | |
− | </div> | + | <div class="printfooter"> |
+ | Retrieved from "<a href= | ||
+ | "https://2013.igem.org/Team:Cornell/notebook">https://2013.igem.org/Team:Cornell/notebook</a>" | ||
+ | and "<a href= | ||
+ | "https://2014.igem.org/Team:NTNU_Trondheim/notebook">https://2014.igem.org/Team:NTNU_Trondheim/notebook</a>" | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div id="catlinks"> | ||
+ | <div class="catlinks catlinks-allhidden" id="catlinks"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
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</html> | </html> |
Revision as of 20:20, 22 June 2015
Notebook
Filter by subteam:
show all subteams
show technical details
Week 18
(28/04 - 04/05)
April 29th
show technical details
Week 23
(02/06 - 08/06)
Week 24
(09/06 - 15/06)
Week 25
(16/06 - 22/06)
Week 26
(23/06 - 29/06)
Week 27
(30/06 - 06/07)
Week 28
(07/07 - 13/07)
July 7th
show technical details
- Amplification of psB1A3 backbone, psB1C3 backbone and psB1K3 backbone using touchdown PCR.
- Digestion, ligation and heat-shock transformed of Left_flank, JB with psB1A3 backbone and Kanamycin_resistance, Righ_flank with psB1A3 backbone.
July 8th
show technical details
- Made LB plates with ampicillin and with chloramphenicol.
- Heat-shock transformed synthesised promoter + RBS region (Construct) into competent E. coli DH5α.
- One colony from each ligation on July 7th were picked and inoculated.
July 9th
show technical details
- Mini-prep of inoculated colonies of Left_flank+JB and Kanamycin_resistance+Righ_flank.
- Digestion, ligation and heat-shock transformation of LFJB, BBa_C0012 and psB1C3 backbone.
- Rehydrated BioBrick BBa_E1010 and heat-shock transformed into competent E. coli DH5α.
July 10th
show technical details
- Gel electrophoresis verification of digest of Left_flank+JB, BBa_C0012, BBa_E1010 and psB1C3 backbone from July 9th on agarose gel using NotI-HF restriction enzyme.
- LFJB on psB1A3 backbone and KRF on psB1A3 backbone sent for sequencing.
- Inoculated new colonies of LFJB and KRF and BBa_E1010 because the gel verification did not give expected bands.
Week 29
(14/07 - 20/07)
Week 30
(21/07 - 27/07)
Week 31
(28/07 - 03/08)
Week 32
(04/08 - 10/08)
Week 35
(25/08 - 31/08)
Week 37
(08/09 - 14/09)
Week 38
(15/09 - 21/09)
Week 39
(22/09 - 28/09)
Week 40
(29/09 - 05/10)