Difference between revisions of "Team:KU Leuven/Research/Results"

The Centre of Microbial and Plant Genetics (KU Leuven) provided us with three E.coli K-12 strains, with each one representing the knockout for the genes tar, tsr or cheZ. The kanamycin cassette of the tar knock-out strain was removed by the enzyme flippase on pCP20. This excision was checked by PCR. Here the original knock-out strain of tar was used as a positive control and a band at 1232 base pairs is expected. If the cassette is removed, a band at 438 base pairs will be visible. Hereby, ten colonies were tested and all have lost their cassette (Figure 1).