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| Line 37: |
Line 37: |
| | <ul type="square"> | | <ul type="square"> |
| | <li> | | <li> |
| − | Wearing lab-grown
| + | Wearing lab-grown |
| | </li> | | </li> |
| | <li> | | <li> |
| − | Wearing lab gloves
| + | Wearing lab gloves |
| | </li> | | </li> |
| | <li> | | <li> |
| − | Using the experimental machine properly
| + | Using the experimental machine properly |
| | | | |
| | <li> | | <li> |
| − | Deal with the waste in a proper manner
| + | Deal with the waste in a proper manner |
| | </li> | | </li> |
| | </ul> | | </ul> |
| Line 97: |
Line 97: |
| | <p class="p1"> | | <p class="p1"> |
| | Environmental Safety | | Environmental Safety |
| − | </p>
| |
| − | <ul type="square">
| |
| − | <li>
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| − | Can be decomposed easily by microorganism in soil
| |
| − | </li>
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| − | <li>
| |
| − | Sensitive to sun light
| |
| − | </li>
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| − | <li>
| |
| − | High selectivity: only have effects on a few certain pests including termites and cotton aphid
| |
| − | </li>
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| − | <li>
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| − | Complete safe to large mammals under recommended concentrations
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| − | </li>
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| − | </ul>
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| − | <p class="p1">
| |
| − | Application Safety
| |
| − | </p>
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| − | <ul type="square">
| |
| − | <li>
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| − | Complete safe to users under recommended concentration
| |
| − | </li>
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| − | <li>
| |
| − | Already existed mature industrial production system
| |
| − | </li>
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| − | <li>
| |
| − | Already have more than 3000 registered avermetin-related products in China, passing the security check of AQSIQ (State General Administration of the People’s Republic of China for Quality Supervision and Inspection and Quarantine)
| |
| − | </li>
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| − | </ul>
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| − | </div>
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| − |
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| − |
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| − | <div class="col-md-6 text-col-right">
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| − |
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| − | <img src="https://static.igem.org/mediawiki/2015/e/e5/ZJU-China_Safety3.png" class="img-center"></img>
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| − | <div class="cpright">
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| − | <p class="kuvateksti">
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| − |
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| − | </p>
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| − | </div>
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| − |
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| − |
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| − | </div>
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| − |
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| − | </div>
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| − |
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| − | <div class="row">
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| − | <p class="p1" style="padding-top:20px;">
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| − | ② Toxic Proteins
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| − | </p>
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| − | <p class="p1">
| |
| − | Because the toxic proteins we use are recently discovered, little related researches can be found online. However, the proteins we use are pretty like bt protein, so we will use the data of bt protein to help us analyze.
| |
| − | </p>
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| − |
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| − | <div class="col-md-6 text-col-left">
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| − |
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| − |
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| − | <p class="p1">
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| − | Environmental Safety
| |
| − | </p>
| |
| − | <ul type="square">
| |
| − | <li>
| |
| − | About 80% can be fast digested in soil or water in 20 days, but will be a little residue (10-20%)
| |
| − | </li>
| |
| − | <li>
| |
| − | Can be absorbed quickly and decomposed easily on the soil surface
| |
| − | </li>
| |
| − | <li>
| |
| − | Will NOT be reproduced in water. Will have some reproduction only in nutrient soil.
| |
| − | </li>
| |
| − | <li>
| |
| − | Non-toxic and non-pathogenic to birds, fish, and shrimp
| |
| − | </li>
| |
| − | <li>
| |
| − | High selectivity: little to no direct toxicity to non-target insects and other shelled invertebrates
| |
| − | </li>
| |
| − | <li>
| |
| − | No adverse effect to rats
| |
| − | </li>
| |
| − | <li>
| |
| − | Will NOT cause a disease outbreak among wild animals
| |
| − | </li>
| |
| − | <li>
| |
| − | Non-toxic to adults and infants
| |
| − | </li>
| |
| − | <li>
| |
| − | No contribution to the development of cancer
| |
| − | </li>
| |
| − |
| |
| − | </ul>
| |
| − | <p class="p1">
| |
| − | Application Safety
| |
| − | </p>
| |
| − | <ul type="square">
| |
| − | <li>
| |
| − | Completely safe to users under recommended concentration
| |
| − | </li>
| |
| − | <li>
| |
| − | Will NOT be used or applied in the wild. Will be completely used in the house.
| |
| − | </li>
| |
| − | <li>
| |
| − | The bacterium are CNC-coated, having little to no leak of the toxic proteins consequently.
| |
| − | </li>
| |
| − | <li>
| |
| − | Completely no harm to adults and infants when users are behaved according to our instruction
| |
| − | </li>
| |
| − | </ul>
| |
| − | </div>
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| − |
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| − |
| |
| − | <div class="col-md-6 text-col-right textcenter">
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| − | <img src="https://static.igem.org/mediawiki/2015/0/0b/ZJU-China_Background2.jpg" class="img-center" style="width:auto;height:347px"></img>
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| − | <div class="cpright">
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| − | <p class="kuvateksti">
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| − |
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| − |
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| − | </p>
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| − | </div>
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| − |
| |
| − |
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| − | </div>
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| − |
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| − |
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| − |
| |
| − |
| |
| − | </div>
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| − |
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| − |
| |
| − | <div id="pos4"></div>
| |
| − | <h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px;margin-top:30px;" id="Experimental">Experimental Safety</h3>
| |
| − | <div class="row">
| |
| − | <div>
| |
| − |
| |
| − | <p class="p1">
| |
| − | During the experiment, we worry that our gene modified bacterium would get out of the lab, thus we figured out several ways to make our bacterium safer.
| |
| − | </p>
| |
| − |
| |
| − | <p class="p1" style="padding-top:20px">
| |
| − | ① Inducible Promoter
| |
| − | </p>
| |
| − | <p class="p1">
| |
| − | In our plan, we shall build four constructs to express toxin proteins that helps killing termites. In case that our bacterium get outside and leak the toxic protein into the environment, we use an inducible promoter in our constructs. Only when there is arabinose in the medium, can the promoter work as usual. There is no arabinose outside the lab, so our bacterium won’t leak the toxic protein.
| |
| − | </p>
| |
| − | <p class="p1">
| |
| − | ② Kill Switch
| |
| − | </p>
| |
| − | <p class="p1">
| |
| − | With regard to this CidA-LrgA system and the BNU iGEM 2014 kill switch design6, we designed our own kill switch as the figure 3 shows. When cultured with our IPTG-added medium, these bacteria would live as usual to function as a mini-factory to amplify plasmids or to produce plasmids for our use, whereas they would die due to the cell membrane lysis and release of genomic DNA. Moreover, we put the lrgA gene under the control of a weak and constitutive promoter J23116 to in case of the leaky expression of the CidA to prevent cell lysis.
| |
| − | </p>
| |
| − | <div class="row">
| |
| − | <div class="col-md-6 text-col-left">
| |
| − | <img src="https://static.igem.org/mediawiki/2015/4/43/ZJU-CHINA_Safety-Stable.png" class="img-center"></img>
| |
| − | <div class="cpright">
| |
| − | <p class="kuvateksti">
| |
| − |
| |
| − | </p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <div class="col-md-6 text-col-right">
| |
| − |
| |
| − | <img src="https://static.igem.org/mediawiki/2015/4/43/ZJU-CHINA_Safety-Stable.png" class="img-center"></img>
| |
| − | <div class="cpright">
| |
| − | <p class="kuvateksti">
| |
| − |
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <div id="pos5"></div>
| |
| − | <h3 style="border-bottom:1px solid #555;font-size:2em;text-align:center;padding-bottom:10px;margin-top:30px;" id="Device" >Device Safety & Social Safety</h3>
| |
| − |
| |
| − | <div class="row">
| |
| − | <div>
| |
| − |
| |
| − | <p class="p1">
| |
| − | In our project, we designed a device aiming at better controlling and killng termites. This device is absolutely not allowed to be used outdoors, but is designed to be used in the house instead. After we designed the device, we are afraid that people might use it in a wrong way after they bought it. In order to avoid people wrongly operating our device, we also design an instruction of our device as below.
| |
| − | </p>
| |
| − | <img src=" https://static.igem.org/mediawiki/2015/c/c5/ZJU-China_Device7.jpg" class="img-center"></img>
| |
| − | <div class="cpleft">
| |
| − | <p class="kuvateksti">
| |
| − |
| |
| − | </p>
| |
| − | </div>
| |
| − | <p class="p1">
| |
| − | We plan to sell our device with the instruction attached to it, and we believe that our device would be safer with our well-designed instruction.
| |
| − | </p>
| |
| − | <p class="p1">
| |
| − | Furthermore, we considered the social safety of our whole project. According to the chart we show below, we considered every aspect to confirm that our project are safe under given conditions..
| |
| − | </p>
| |
| − | <img src="https://static.igem.org/mediawiki/2015/e/ec/SAFETY.gif" class="img-center"></img>
| |
| − | <div class="cpleft">
| |
| − | <p class="kuvateksti">
| |
| − |
| |
| − | </p>
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | </article>
| |
| − |
| |
| − | </div>
| |
| − |
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| − |
| |
| − |
| |
| − | <!--------------------------------------------------------------------------->
| |
| − | </body>
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| − |
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| − |
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| − |
| |
| − | </html>
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| − | {{ZJU-China/mascot}}
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| − | {{:Team:ZJU-China/template/foot}}
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