Difference between revisions of "Team:KU Leuven/InterLabStudy/Results"
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Revision as of 14:34, 16 September 2015
Interlab Results
The results of our Interlab study are discussed in this section The minipreped samples of the given devices were validated by restriction mapping using the enzymes NcoI and XhoI. These restriction enzymes left us with bands around 267, 364, 625 and 1724 basepairs. This was validated by a gel electrophoresis (figure 1). The numbers 101, 106 and 117 stand for the devices containing the promoters J23101, J23106 and J23117 respectively.
Figure 1
Restriction digest of the three devices with NcoI and XhoI.
The restriction digest (Fig 1) shows our devices containing GFP I13504 and the promoters J23101, J23106 and J23117.The expected bands were at 267, 364, 625, 1724 bp respectively.
We made a Fluorescein standard graph and extrapolated the concentration of the GFP from the samples using the fluorescence and the absorbance values that were recorded. We went ahead with those values to calculate the mean and the standard deviation for our biological and technical replicates. We processed all the data in Microsoft excel.
Table 1:The raw fluorescence data ( excitation at 483 nm and emission at 525 nm) for LB medium containing chloramphenicol, LB medium with chloramphenicol and cells containing the biobrick J3101 and the three devices (D1, D2, D3). Biological replicates originating from three different devices are presented in the rows and the technical replicates for the devices are presented in the columns.
| LB+Cam | LB+Cam+Cells | D1 | D2 | D3 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 512 | 601 | 737 | 685 | 751 | 9425 | 9322 | 9737 | 22786 | 25895 | 25048 |
| 546 | 594 | 754 | 641 | 641 | 9669 | 9545 | 9876 | 23159 | 26460 | 24785 |
| 549 | 587 | 722 | 660 | 697 | 9407 | 9321 | 9677 | 22719 | 25931 | 24935 |
| Value | Value | Value | Value | Value | Value | Value | Value | Value | Value | Value |
Figure 1
Restriction digest of the three devices with NcoI and XhoI.
Figure 2 Left: plasmid A, Right: plasmid B. Click to enlarge
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be
