Difference between revisions of "Team:BostonU/Interlab"
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<p>Our team participated in the interlab study this year. The interlab study is coordinated effort by iGEM to | <p>Our team participated in the interlab study this year. The interlab study is coordinated effort by iGEM to | ||
gather fluorescence data from three known genetic devices across different labs from around the world. | gather fluorescence data from three known genetic devices across different labs from around the world. | ||
Revision as of 15:52, 16 September 2015
Interlab Study
Our team participated in the interlab study this year. The interlab study is coordinated effort by iGEM to
gather fluorescence data from three known genetic devices across different labs from around the world.
This is an effort to normalize fluorescence data that may have been recorded under different conditions.
Our team used biobrick cloning methods to make the constructs each of which consisted of a GFP gene
under a different constitutive promoter. The data was collected using FACS. The data was then
processed by negating all fluorescence from cells that were outliers in size or complexity. Then, all cells
that expressed a negative fluorescence value were disregarded.
