Difference between revisions of "Team:Warwick/Collaborations"

Revision as of 17:18, 16 September 2015

Warwick iGEM 2015

Collaborations

Glasgow Collaboration

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Manchester-Graz Collaboration

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NTNU Modelling Collaboration

After we came up with a model of DNA Origami arms and the sequences for them and how they would bond we wanted to model the probability of the E.coli arms forming fully and how this probability would change as length of the arms increased. We came up with a basic model but thought it would be beneficial if we sought outside help with the mathematics.
We got in contact with NTNU to ask for assistance and they came back with a stochastic method for calculating said probabilities. After discussing the problem further we came up with various equations such as S_i= Σ_j (K_i,j / Σ_j K_i,j ) log K_i,j / E_j[K_i,j], where S is how well a zinc finger binds to an arm and P_i = S_i / W (S_i) where P is the probability of formation for that zinc finger arm.

Oxford Collaboration

One part of our project was create DNA Origami arms using a part from the distributed kit. Once we had the design made, we decided to collaborate with Oxford to help get it made.
We worked with them to PCR a part we had designed using primers we had sequenced. This allowed us to use our time more efficiently and begin work on the second phase of the project.
Once we got the parts back we combined them and annealed to form DNA Origami shapes, which were then viewed under an electron microscope.

Design by Warwick iGEM 2015