Difference between revisions of "Team:Paris Saclay/Notebook/July/1"
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''by Seong Koo, Pauline, Johan'' | ''by Seong Koo, Pauline, Johan'' | ||
| − | Preparation | + | Preparation competent cells DH5α with CaCl2 cf:protocol |
15mL competente cells | 15mL competente cells | ||
| − | Aliquot the solution in chilled 1.5mL microcentrifuge tubes ( | + | Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500µL/tube) and frozen and store at -70°C |
===Preparation Petri dishes with LB=== | ===Preparation Petri dishes with LB=== | ||
Latest revision as of 17:38, 16 September 2015
Contents
July 1
Lab Work
Preparation
by Johan
Plasmid Rehydratation :
by Johan Measure fluorescence : I13504 J23117 J23106 J23101
For our project : R0051 J13602 J13522 C0040
Preparation of liquid culture of DH5α with K115017
K115017 5mL LB + 5uL Cm (20mg/mL)
Competente cells preparation
by Seong Koo, Pauline, Johan
Preparation competent cells DH5α with CaCl2 cf:protocol
15mL competente cells Aliquot the solution in chilled 1.5mL microcentrifuge tubes (500µL/tube) and frozen and store at -70°C
Preparation Petri dishes with LB
by Seong Koo and Pauline
22 Petri dishes with LB + Cm (20µL/mL) 12 Petri dishes with LB + Amp (100µL/mL)
Members present:
- Instructors : Alice.
- Students : Johan, Seong Koo, Pauline.
Contact
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. All rights reserved All inspirations encouraged.
*This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.
eigem.parissaclay AT gmail DOT com
t@iGEMParisSaclay
aUniversité Paris Sud
a91405 Orsay, France
Copyright © 2015 iGEM Paris Saclay. *This wiki was conceived during long nights of work and a great coffee experience in the beautiful Orsay, France.