|
|
| Line 1: |
Line 1: |
| | | | |
| − | {{Warwick}}
| |
| − | <html>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <!-- SUBHEADER
| |
| − | ================================================== -->
| |
| − | <a id="link1"></a>
| |
| − | <div id="subheader">
| |
| − | <div class="row">
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − | <div class="hr">
| |
| − | </div>
| |
| − | </div>
| |
| − | <!-- CONTENT
| |
| − | ================================================== -->
| |
| − | <div class="row">
| |
| − | <!-- MAIN CONTENT-->
| |
| − | <div class="eight columns">
| |
| − | <div class="sectiontitle">
| |
| − | <h4>DNA Origami Glue Sequences</h4>
| |
| − | </div>
| |
| − | <!-- Services List-->
| |
| − | <div class="fifteen columns noleftmargin">
| |
| − |
| |
| − | <p>
| |
| − | <img src="https://static.igem.org/mediawiki/2015/7/7d/Warwick3armedglue.png" class="pics" alt="" height="700px" width="980px">
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <h5 class="sidebartitle">BBa_K314110</h5>
| |
| − | <p>
| |
| − | <p style="float: right;"><img src="https://static.igem.org/mediawiki/2015/c/c8/WarwickSequencesfor3.png" align="right" height="300px" width="440px" border="1px"></p>
| |
| − | The right shows the sequences and linkers which we will use.
| |
| − | <br><b>Procedure</b>
| |
| − | <br>1) Remove insert from plasmid
| |
| − | <br>2) PCR the plasmids
| |
| − | <br>3) Use restriction enzymes to cut the plasmids into smaller pieces of equal length (150 bp)
| |
| − | <br>4) Create "linker" DNA
| |
| − | <br>5) Denature the double stranded DNA and add linkers
| |
| − | <br>6) Allow to cool and be amazed at the results
| |
| − |
| |
| − | <p style="float: right;">
| |
| − |
| |
| − | <p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/2/25/WarwickPrimersequencesfory.png" align="right" height="400px" width="640px" border="1px"></p>
| |
| − | </p>
| |
| − |
| |
| − |
| |
| − |
| |
| − |
| |
| − | <p style="float: right;">
| |
| − | </p>
| |
| − | </div>
| |
| − |
| |
| − | </p>
| |
| − | </div>
| |
| − | </div><!-- end main content-->
| |
| − |
| |
| − | <!-- SIDEBAR-->
| |
| − |
| |
| − | </div>
| |
| − | <div class="hr">
| |
| − | </div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | </html>
| |
| − |
| |
| − | {{WarwickFooter}}
| |