Difference between revisions of "Team:Wellesley TheTech/Project/Wetlab"
| Line 55: | Line 55: | ||
-They can see a photo of their grown ‘mars bacteria’ several days later<br> | -They can see a photo of their grown ‘mars bacteria’ several days later<br> | ||
</p> | </p> | ||
| − | <br> | + | <hr> |
| − | + | <h3>Development</h3> | |
| + | <p> | ||
| + | Plasmids: ADD HYPERLINKS<br> | ||
| + | Selected 5 bioBricks from the iGEM distribution kit: <br> | ||
| + | -Lux Operon (BBa_K325909) - <a href="http://parts.igem.org/Part:BBa_K325909">Bioluminescence pathway</a><br> | ||
| + | amilGFP (BBa_K592010) - Yellow chromoprotein<br> | ||
| + | aeBlue (BBa_K864401) - Blue chromoprotein<br> | ||
| + | eforRed (BBa_K592012) - Red/pink chromoprotein<br> | ||
| + | asPink (BBa_K1033933) - Pink/purple chromoprotein<br> | ||
| + | </p> | ||
</div> | </div> | ||
Revision as of 18:12, 16 September 2015
WETLAB COMPONENT
Goal
The goal of the wetlab component of BacPack is to connect the digital experience to a hands-on, lab-based one. In the digital activity visitors explore designing bacteria to have a variety of new, useful functions for helping humans survive in different extreme environments. In the wetlab activity visitors get try their hands at the same real-world process of engineering a bacteria to have a new property of their choosing.
Activity Design Outline
Visitors transform a living bacteria to make it do something new:
-5 DNA options that change the physical appearance of bacteria in different ways
-Each ‘color’ represents a bacteria function that can provide one of the 5 needs on Mars
-Visitors pick the DNA they want to use and transform e.coli with it
-They can see a photo of their grown ‘mars bacteria’ several days later
Development
Plasmids: ADD HYPERLINKS
Selected 5 bioBricks from the iGEM distribution kit:
-Lux Operon (BBa_K325909) - Bioluminescence pathway
amilGFP (BBa_K592010) - Yellow chromoprotein
aeBlue (BBa_K864401) - Blue chromoprotein
eforRed (BBa_K592012) - Red/pink chromoprotein
asPink (BBa_K1033933) - Pink/purple chromoprotein