Difference between revisions of "Team:Warwick/Microscopy"
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<p>We set out to prove this using fluorescein bound oligonucleotides that contained our zinc finger target DNA sequence. By fixing different cells to slides and washing them with different oligos we could determine whether the zinc fingers bind only target DNA, or any DNA. This test could be conducted quantitatively by doing a photon count using the fluorescent microscope. This can then be used to determine which of the four zinc fingers we tested bound the most oligo, and therefore had the strongest binding affinity with its target sequence. This information could potentially be important for any further research conducted in this field. </p> | <p>We set out to prove this using fluorescein bound oligonucleotides that contained our zinc finger target DNA sequence. By fixing different cells to slides and washing them with different oligos we could determine whether the zinc fingers bind only target DNA, or any DNA. This test could be conducted quantitatively by doing a photon count using the fluorescent microscope. This can then be used to determine which of the four zinc fingers we tested bound the most oligo, and therefore had the strongest binding affinity with its target sequence. This information could potentially be important for any further research conducted in this field. </p> | ||
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Revision as of 18:21, 16 September 2015
