Difference between revisions of "Team:Wellesley TheTech/Project/Wetlab"
| Line 58: | Line 58: | ||
<h3>Development</h3> | <h3>Development</h3> | ||
<p> | <p> | ||
| − | Plasmids: | + | <b>Plasmids:</b><br> |
| − | Selected 5 bioBricks from the iGEM distribution kit: <br> | + | <b>Selected 5 bioBricks from the iGEM distribution kit:</b> <br> |
| − | -Lux Operon (BBa_K325909) - <a href="http://parts.igem.org/Part: | + | -Lux Operon (BBa_K325909) - <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K592010" target="_blank" style="color:blue">Bioluminescence pathway</a><br> |
| − | amilGFP (BBa_K592010) - Yellow chromoprotein<br> | + | amilGFP (BBa_K592010) - <a href="http://parts.igem.org/Part:BBa_K325909" target="_blank" style="color:blue">Yellow chromoprotein</a><br> |
| − | aeBlue (BBa_K864401) - | + | aeBlue (BBa_K864401) - <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K864401” target="_blank" style="color:blue">Blue chromoprotein</a><br> |
| − | eforRed (BBa_K592012) - Red/pink chromoprotein<br> | + | eforRed (BBa_K592012) - <a href="http://parts.igem.org/wiki/index.php/Part:BBa_K592012” target="_blank" style="color:blue">Red/pink chromoprotein</a><br> |
| − | asPink (BBa_K1033933) - Pink/purple chromoprotein<br> | + | asPink (BBa_K1033933) - <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1033933” target="_blank" style="color:blue">Pink/purple chromoprotein</a><br> |
</p> | </p> | ||
</div> | </div> | ||
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</div> | </div> | ||
</html> | </html> | ||
Revision as of 18:29, 16 September 2015
WETLAB COMPONENT
Goal
The goal of the wetlab component of BacPack is to connect the digital experience to a hands-on, lab-based one. In the digital activity visitors explore designing bacteria to have a variety of new, useful functions for helping humans survive in different extreme environments. In the wetlab activity visitors get try their hands at the same real-world process of engineering a bacteria to have a new property of their choosing.
Activity Design Outline
Visitors transform a living bacteria to make it do something new:
-5 DNA options that change the physical appearance of bacteria in different ways
-Each ‘color’ represents a bacteria function that can provide one of the 5 needs on Mars
-Visitors pick the DNA they want to use and transform e.coli with it
-They can see a photo of their grown ‘mars bacteria’ several days later
Development
Plasmids:
Selected 5 bioBricks from the iGEM distribution kit:
-Lux Operon (BBa_K325909) - Bioluminescence pathway
amilGFP (BBa_K592010) - Yellow chromoprotein
aeBlue (BBa_K864401) - Blue chromoprotein
eforRed (BBa_K592012) - Red/pink chromoprotein
asPink (BBa_K1033933) - Pink/purple chromoprotein