Difference between revisions of "Team:Warwick/Cloning"

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<h4>Cloning and Transformation</h4>
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<h1>Cloning and Transformation</h1>
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Our project relied greatly on previously established techniques for cloning modified genes into plasmids and transforming those plasmids into competent cells. We wanted to make our part as modular and easy to change as possible, meaning we had to rely heavily on techniques such as Gibson assembly to construct our plasmid.
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By offering our part with changeable parts we hoped to make our part more useful for further research, and allow for deeper applications than the ones we initially thought of. Having interchangeable zinc fingers allows the diversity of the cells that can be glued together using our system to be much greater by allowing each different cell type or individual cell to have a specific DNA sequence that it binds to, so that fine control of spatial positioning of cells can be achieved.
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<img src="https://static.igem.org/mediawiki/2015/1/1d/Warwick_Modularity.png" align="middle">
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Revision as of 18:33, 16 September 2015

Warwick iGEM 2015