Difference between revisions of "Team:UiOslo Norway/Experiments/Dialysis"

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Revision as of 20:15, 16 September 2015

Dialysis

Back to Protocols


  • Combine the elution fractions that are pure and containing the protein of interest.

  • Dialyse against dialysis buffer for 2 hours and change the buffer after 1 hour.

    • Dialysis buffer

    20 mM Tris-HCl pH 8
    50 mM NaCl
    0.5 mM EDTA
    10 mM beta-Mercaptoethanol

    iGEM UiOslo 2015 was sponsored by: