Difference between revisions of "Template:IONIS Paris/Notebook/09-09-15"

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<i class="fa fa-angle-right"></i> Measurements of DO595 and DO405 (wtGFP) all the day long <br>
 
<i class="fa fa-angle-right"></i> Measurements of DO595 and DO405 (wtGFP) all the day long <br>
<i class="fa fa-caret-right"></i> Typical profile of colonies transformed with pDawn-HOKD under light exposition<br><br>
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<h5 style="color:rgba(255, 0, 0, 0.6);font-weight: bold;">Typical profile of colonies transformed with pDawn-HOKD under light exposition</h5>
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<center><img src="https://static.igem.org/mediawiki/2015/a/af/Res0909.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br>
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<i class="fa fa-angle-right"></i>Continuous bacterial growth despite light exposure <i class="fa fa-caret-right"></i> not expected
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<h5 style="color:rgba(255, 0, 0, 0.6);font-weight: bold;"> Typical profile of colonies transformed with pDawn-CCDB under light exposition:<h5>
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<center><img src="https://static.igem.org/mediawiki/2015/a/af/Res0909.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br>
 
<center><img src="https://static.igem.org/mediawiki/2015/a/af/Res0909.png" alt="img01" class="img-responsive" style="width:50%;height:50%" /></center><br>
 
<i class="fa fa-angle-right"></i>Continuous bacterial growth despite light exposure <i class="fa fa-caret-right"></i> not expected
 
<i class="fa fa-angle-right"></i>Continuous bacterial growth despite light exposure <i class="fa fa-caret-right"></i> not expected
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Revision as of 21:37, 16 September 2015

E.coli DH5 alpha transformation

Transformation of competent cells with pSB1C3-GFP and pSB1C3-H/E (ratio 3:1, 1:1, 1:3) with 3 µl of plasmid.

Liquid cultures on plates from 08/09

Measurements of DO595 and DO405 (wtGFP) all the day long

Typical profile of colonies transformed with pDawn-HOKD under light exposition

img01

Continuous bacterial growth despite light exposure not expected

Typical profile of colonies transformed with pDawn-CCDB under light exposition:

img01

Continuous bacterial growth despite light exposure not expected


9 September 15