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Attributions

Despite our tireless efforts we could not have done this on our own. Therefore we would like to express our gratitude and thank all on the following lists.

Sponsors

• University of Southern Denmark

• Department of Microbiology under The Deparment of Biochemistry and Molecular Biology - for providing us laboratory space, equipment and always being patient and accessible.

Laboratory support

• Our instructors Postdoc and Ph. D. Mikkel Girke Jørgensen, Academic Assistant Tina Kronborg, Ph. D. student Thøger Jensen Krogh and Stud.cand.scient Patrick Rosendahl Andreassen
• Medical Laboratory Technician Simon Rose who

General support

• DTU – for arranging the anually bio brick workshop
• Vilnius iGEM team – for hosting our visit for three days
• The SDU iGEM team 2013
• Uppsala iGEM team - Nordic Meetup
• Associate professor, MD Søren Thue Lillevang. Department of Clinical Immunology, Odense University Hospital. He attributed to the discussion about medical aspects
• Associate Professor Jan Nehlin, Department of Clinical Immunology Odense University Hospital.
• Nancy Rasmussen - for helping us proof reading our wiki.

Technical support

Litterature support

Antibody issue

• Lubert Stryer JMB, John L. Tymoczko. The Immune System. Biochemistry. 7 ed: W. H. Freeman and Company; 2012.
• Cowan MK. Host Defenses II - Specific Immunity and Immunization. Microbiology - A Systems Approach. 3 ed: McGraw-Hill; 2012.
• Chippaux JP, Goyffon M. Venoms, antivenoms and immunotherapy. Toxicon. 1998;36(6):823-46.
• Clem AS. Fundamentals of vaccine immunology. J Glob Infect Dis. 2011;3(1):73-8.
• Siddiqui MZ. Monoclonal antibodies as diagnostics; an appraisal. Indian J Pharm Sci. 2010;72(1):12-7.
• Berger M, Shankar V, Vafai A. Therapeutic applications of monoclonal antibodies. Am J Med Sci. 2002;324(1):14-30.
• Scott AM, Wolchok JD, Old LJ. Antibody therapy of cancer. Nat Rev Cancer. 2012;12(4):278-87.
• BioPharma P. What Are Humanized Monoclonal Antibodies 2015 [cited 2015 08/08]. (Link)
• Payne WJ, Jr., Marshall DL, Shockley RK, Martin WJ. Clinical laboratory applications of monoclonal antibodies. Clin Microbiol Rev. 1988;1(3):313-29.
• Hnasko RM, Stanker LH. Hybridoma Technology. Methods Mol Biol. 2015;1318:15-28.
• Stills HF, Jr. Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants. ILAR journal / National Research Council, Institute of Laboratory Animal Resources. 2005;46(3):280-93.
• Descartes Rene. Les Méditations métaphysiques’, 1647
• Bentham Jeremy, Introduction to the Principles of Morals and Legislation, 1789
• Three RS (Link)
• Cruelty Free International (Link)
• Nelson PN, Reynolds GM, Waldron EE, Ward E, Giannopoulos K, Murray PG. Demystified …: Monoclonal antibodies. Molecular Pathology. 2000;53(3):111-7.
• Colas P. The eleven-year switch of peptide aptamers. Journal of biology. 2008;7(1):2.
• Colas P, Cohen B, Jessen T, Grishina I, McCoy J, Brent R. Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2. Nature. 1996;380(6574):548-50.
• Borghouts C, Kunz C, Delis N, Groner B. Monomeric recombinant peptide aptamers are required for efficient intracellular uptake and target inhibition. Molecular cancer research : MCR. 2008;6(2):267-81.
• Evans D, Johnson S, Laurenson S, Davies AG, Ko Ferrigno P, Wälti C. Electrical protein detection in cell lysates using high-density peptide-aptamer microarrays. Journal of biology. 2008;7(1):3.
• Li J, Tan S, Chen X, Zhang CY, Zhang Y. Peptide aptamers with biological and therapeutic applications. Current medicinal chemistry. 2011;18(27):4215-22.
• Swiss Institute of Bioinformatics. 2015. (Link)
• Key Benefits: Affimers have some simple but essential advantages over antibodies: Avacta Life Sciences; [updated 19-08-2015]. (Link)

P.A.S.T

• Karimova G, Pidoux J, Ullmann A, Ladant D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.(Link)
• Battesti A, Bouveret E. The bacterial two-hybrid system based on adenylate cyclase reconstitution in Escherichia coli. Methods (San Diego, Calif). 2012;58(4):325-34.(Link)
• SIMON L. DOVE, J. KEITH JOUNG* & ANN HOCHSCHILD - ‘Activation of prokaryotic transcription through arbitrary protein–protein contacts’ - Nature 386, 627-630 (10 April 1997).(Link)
• J. Keith Joung, Elizabeth I. Ramm, and Carl O. Pabo - ‘A bacterial two-hybrid selection system for studying protein–DNA and protein–protein interactions’ - Proceedings of the National Academy of Science of the United States. vol. 97, (20 june 2000).(Link)

Process

• World health organization, third edition, 2004 - Visited: 16.09.15.(Link)
• Lov om miljø og genteknologi - Visited: 16.09.15.(Link)
• Arbejdstilsynet - Visited: 16.09.15.(Link)
• E.coli Genome Project, University of Wisconsin - Visited: 16.09.15.(Link)
• NIH Guidelines for research involving - Visited: 16.09.15.(Link)
• WO 2001073108 A2 - Patent - Visited: 16.09.15.(Link)
• Europa-parlementets og rådets direktiv 2009/41/EF - Visited: 16.09.15.(Link)

Results

Practices

What's Next

• Karimova G, Pidoux J, Ullmann A, Ladant D. - A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(10):5752-6.(Link)