Difference between revisions of "Team:Penn/Sender"

Line 91: Line 91:
 
     <p>An effective light-based communication system rests on the bioluminesence generated by the “sender cell.”  In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909). </p>
 
     <p>An effective light-based communication system rests on the bioluminesence generated by the “sender cell.”  In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909). </p>
  
−
<div><a href="https://2015.igem.org/Team:Penn/Educational_Toolbox"><img src="https://static.igem.org/mediawiki/2015/6/6b/Luminesence_Graph1.png" alt></a></div>
+
     <p class="margin-top-10">Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.</p>
−
 
+
−
     <p class="margin-top-10">When the New York fashion community notices your brand, the world soon follows. The widespread love for UGG extended to Europe in the mid-2000's along with the stylish casual movement and demand for premium casual fashion. UGG boots and shoes were now seen walking the streets of London, Paris and Amsterdam with regularity. To meet the rising demand from new fans, UGG opened flagship stores in the UK and an additional location in Moscow. As the love spread farther East, concept stores were opened in Beijing, Shanghai and Tokyo. UGG Australia is now an international brand that is loved by all. This love is a result of a magical combination of the amazing functional benefits of sheepskin and the heightened emotional feeling you get when you slip them on your feet. In short, you just feel better all over when you wear UGG boots, slippers, and shoes.</p>
+
  
 
<p class="line-break margin-top-10"></p>
 
<p class="line-break margin-top-10"></p>

Revision as of 23:26, 16 September 2015

PENN iGEM 2015



SENDER

An effective light-based communication system rests on the bioluminesence generated by the “sender cell.” In order to design a well-functioning system, the Penn 2015 iGEM team worked to optimize the light output of various E.coli “sender cells” transformed with the lux operon (BBa_K325909).

Lux operon expression is responsible for bioluminescence. The operon is initiated by a constitutive promoter (BBa_J23100) followed by an RBS + lux box. The box contains the following: LuxC, D, A, B, E and G. LuxA and B encode two subunits of bacterial luciferase. The genes LuxC, D, and E drive expression of the substrate for the light-emitting reaction, tetradecanal. The function of the luxG gene is yet to be fully elucidated; however, inclusion of the gene is known to increase light output (CITATION). The circuit is completed with a stop codon and a terminator sequence.