Difference between revisions of "Team:Georgia State/Collaborations"
| Line 219: | Line 219: | ||
$0.0715/gram x 10 = $0.715 <br> | $0.0715/gram x 10 = $0.715 <br> | ||
<b><i>Total = $7.815</i></b> | <b><i>Total = $7.815</i></b> | ||
| + | |||
| + | <h4>J-broth(1L)</h4> | ||
| + | Bulk Supplements Amino Acids - $16.96 for 250 grams | ||
| + | $0.067/gram x 10 =$0.67<br> | ||
| + | Marmite Yeast Extract - $12.63 for 500 grams | ||
| + | $0.025/gram x 5 = $0.125<br> | ||
| + | Sodium Chloride (table salt) - $8.28 for 1000grams | ||
| + | $0.0083/gram x 10 = $0.083<br> | ||
| + | |||
| + | <b><i>Total = $0.275</i></b> | ||
Revision as of 01:02, 17 September 2015
Collaborations
Backyard Biology @ Emory University: Project JBroth
Low Cost LB and Competent Cells!
Our interest in our topic began when we were researching possible projects. What we found most interesting was a research paper from the Journal of Pakistan Medical Association that tested the efficacy of microwaving LB rather than autoclaving. Also, a project that inspired us was the project from the University of Calgary (2014) that tested the efficacy of a homemade wash for competent cells. From these projects, we asked ourselves: Could one conduct a synthetic biology project without access to a professional laboratory?
This summer, we created a cost effective alternative to lab grade Luria Broth (LB). To compare our homemade broth, J-Broth, and lab grade LB we compared optical densities of liquid cultures and transformation efficiency of competent cells. For each method of testing, we compared autoclaved LB, microwaved LB, and microwaved J-Broth.
Materials/Methods
We grew XL1-Blue E. coli cells in liquid culture of lab grade luria broth and J-broth. The media was either autoclaved or microwaved for 8 minutes at 650 Watts before the cells were added.
We then made competent cells using XL1-Blue e. coli cells that were grown in each type of media following the Inoue method (Inoue, 1990) and calculated the transformation efficiency for comparison.
