Difference between revisions of "Team:Virginia/Parts"

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</div></a> <!-- END Part 1 --><!-- BEGIN Part 2 --><tr>
 
</div></a> <!-- END Part 1 --><!-- BEGIN Part 2 --><tr>
 
<td>
 
<td>
<p> IPTG Promoter+RBS+EIIA+T </p>
+
<p> Promoter+RBS+GlgC+TT </p>
 
</td>
 
</td>
 
<td>
 
<td>
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</td></tr>
 
</td></tr>
 
<a href="javascript:void(0);"> <div class="popup" id="popup-2">
 
<a href="javascript:void(0);"> <div class="popup" id="popup-2">
<h4>IPTG Promoter+RBS+EIIA+TT</h4>
+
<h4>Promoter+RBS+GlgC+TT</h4>
<p> BioBrick BBa_1600001serves to increase the uptake of glucose by the cell. This extra glucose will then be
+
<p> BioBrick BBa_1600002 is one of our polymerization circuits and serves to polymerize glucose into the  
  
polymerized by one of our following BioBrick devices (BBa_ . This composite BioBrick is composed  
+
polysaccharide glycogen in order to increase sugar absorption time thus minimizing the probability of  
 +
 
 +
glycemic spikes. This composite BioBrick is composed of BBa_J23110 (promoter), BBa_K118018 (RBS and
 +
 
 +
Glgc), and BBa_B0015 (double terminator).
  
of BBa_K215000 (IPTG promoter), BBa+R0034 (RBS) and BBa_K1508003 (EIIA plus promoter).
 
 
  </p>
 
  </p>
 
<img src="#"></img>
 
<img src="#"></img>
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<!-- BEGIN Part 1 --><tr>
 
<!-- BEGIN Part 1 --><tr>
 
<td>
 
<td>
<p> IPTG Promoter+RBS+EIIA+T </p>
+
<p> Promoter+RBS+SacB+TT </p>
 
</td>
 
</td>
 
<td>
 
<td>
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<a href="javascript:void(0);"> <div class="popup" id="popup-3">
 
<a href="javascript:void(0);"> <div class="popup" id="popup-3">
<h4>IPTG Promoter+RBS+EIIA+TT</h4>
+
<h4>Promoter+RBS+SacB+TT</h4>
<p> BioBrick BBa_1600001serves to increase the uptake of glucose by the cell. This extra glucose will then be
+
<p> BioBrick BBa_1600003 is another of our polymerization circuits and is important not only for
  
polymerized by one of our following BioBrick devices (BBa_ . This composite BioBrick is composed  
+
polymerization of levan but also acts as our kill switch which is activated once there is greater than 5%
 +
 
 +
of fructose in the cell. This serves as an important aspect of our safety factor since this kill switch is
 +
 
 +
responsible for the lysing of the cell to prevent colonization of antibiotic resistant bacteria in the gut.
 +
 
 +
This composite BioBrick is composed of BBa_J23110 (promoter), BBa_K322921 (RBS and SacB) and
 +
 
 +
BBa_B0015 (double terminator).
  
of BBa_K215000 (IPTG promoter), BBa+R0034 (RBS) and BBa_K1508003 (EIIA plus promoter).
 
 
  </p>
 
  </p>
 
<img src="#"></img>
 
<img src="#"></img>
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<!-- BEGIN Part 1 --><tr>
 
<!-- BEGIN Part 1 --><tr>
 
<td>
 
<td>
<p> IPTG Promoter+RBS+EIIA+T </p>
+
<p> Promoter+RBS+GlgC+RBS+SacB+TT </p>
 
</td>
 
</td>
 
<td>
 
<td>
Line 113: Line 123:
  
 
<a href="javascript:void(0);"> <div class="popup" id="popup-4">
 
<a href="javascript:void(0);"> <div class="popup" id="popup-4">
<h4>IPTG Promoter+RBS+EIIA+TT</h4>
+
<h4>Promoter+RBS+GlgC+RBS+SacB+TT</h4>
<p> BioBrick BBa_1600001serves to increase the uptake of glucose by the cell. This extra glucose will then be
+
<p> BioBrick BBa_1600004 is our composite polymerization circuit combining the functions of BBa_1600002
 +
 
 +
and BBa_1600003. This BioBrick polymerizes glucose into glycogen, polymerizes levan from fructose,
 +
 
 +
and possesses the kill switch responsible for lysing the cell. This composite BioBrick is composed of
 +
 
 +
BBa_J23110 (promoter), BBa_K118018(RBS and Glgc), BBa_K322921 (RBS and SacB) and BBa_B0015
  
polymerized by one of our following BioBrick devices (BBa_ . This composite BioBrick is composed
+
(double terminator).
  
of BBa_K215000 (IPTG promoter), BBa+R0034 (RBS) and BBa_K1508003 (EIIA plus promoter).
 
 
  </p>
 
  </p>
 
<img src="#"></img>
 
<img src="#"></img>

Revision as of 01:06, 17 September 2015

University of Virginia iGEM 2015

IPTG Promoter+RBS+EIIA+T

BBa_XXXXX

Promoter+RBS+GlgC+TT

BBa_XXXXX

Promoter+RBS+SacB+TT

BBa_XXXXX

Promoter+RBS+GlgC+RBS+SacB+TT

BBa_XXXXX

University of Virginia iGEM

148 Gilmer Hall

485 McCormick Road

Charlottesville, Virginia 22904

United States of America

virginia.igem@gmail.com