Difference between revisions of "Team:NTNU Trondheim"

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<h4> NTNU Trondheim iGEM 2015 </h4>
 
<h4> NTNU Trondheim iGEM 2015 </h4>
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<p>NTNU Trondheim is a team from Trondheim, Norway, competing for the iGEM 2015 student competition. NTNU Trondheim maintains the <a href="http://almaaslab.nt.ntnu.no/igem_matchmaker/">iGEM Matchmaker Tool</a> which helps iGEM teams cooperate with each other.
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<p>NTNU Trondheim is a team from Trondheim, Norway, competing for the iGEM 2015 student competition with glucose-sensing alginate-encapsulate <i>Pseudomonas Putida</i>. NTNU Trondheim maintains the <a href="http://almaaslab.nt.ntnu.no/igem_matchmaker/">iGEM Matchmaker Tool</a> which helps iGEM teams cooperate with each other.
 
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<p>We plan to engineer the soil bacterium <i>Pseudomonas putida</i> to function as a glucose detector. In the presence of glucose it will express the red fluorescent protein mCherry (see <strong>Figure 1</strong>). To achieve this we will use the promoters of four operons commonly found in <i>Pseudomonas putida</i>. The operons are negatively controlled, and the expression of subsequent genes is initiated by derivatives of glucose. We will characterize the performance of these four promoters and choose one to do further work on.
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<p>Further, we will encapsulate our engineered bacteria in alginate. This is a polymeric biomaterial from brown algae that can be used to form gel capsules. By encapsulating living cells in alginate, the semi permeable gel capsule will shield the cells from the surroundings at the same time as letting small molecules like glucose in and out (<strong>Figure 1</strong>).
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<a href="https://2014.igem.org"><img src="https://static.igem.org/mediawiki/2014/a/af/Igemlogo_officiallogo_no_text_ntnu_logo_blue_border_3.png" width="500px"></a>
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  <img class="img-responsive" src="https://static.igem.org/mediawiki/2015/0/0c/NTNU_Trondheim_Scheme.png" alt="Glucose metabolism"><br>
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  <figcaption><strong>Figure 1</strong> - Alginate encapsulated <i>Pseudomonas putida</i> will express the red fluorescent protein mCherry upon glucose detection.</figcaption>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Project"><img src="https://static.igem.org/mediawiki/2014/9/96/NTNU_lab_small.jpg"></a>
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<p><a href="https://2015.igem.org/Team:NTNU_Trondheim/Description">Read more...</a></p>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Notebook"><h3 style="margin:0px auto 0px;">Project</h3></a>
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We have constructed BioBricks that allow the insertion of transgenes into <i>Synechocystis</i> sp. PCC 6803. Working with photosynthetic organisms such as <i>Synechocystis</i> can help in the generation of environmental innovations.  
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We hope that our BioBricks can encourage and make it easier for future teams to work with <i>Synechocystis</i> and other photosynthetic organisms.<br><br><br><br>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Notebook" class="small button radius corner">More Info</a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Judging"><img src="https://static.igem.org/mediawiki/2014/7/72/Checkbox.png"></a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Judging"><h3 style="margin:0px auto 0px;">Judging criteria</h3></a>
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See which judging criteria we have met.
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Judging" class="small button radius">More Info</a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Notebook"><img src="https://static.igem.org/mediawiki/2014/4/40/NTNU_gel.jpg"></a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Notebook"><h3 style="margin:0px auto 0px;">Notebook</h3></a>
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See our day to day activities in the lab.
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Notebook" class="small button radius corner">More Info</a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Team"><img src="https://static.igem.org/mediawiki/2014/b/b8/IGEM_team.jpg" style="height:120px"></a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Team"><h3 style="margin:0px auto 0px;">Team</h3></a>
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Meet the team behind NTNU's 2014 project.
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Team" class="small button radius corner">More Info</a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Protocols"><img src="https://static.igem.org/mediawiki/2014/3/3d/NTNU_pippette_hand.jpg"></a>
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Protocols"><h3 style="margin:0px auto 0px;">Protocols</h3></a>
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Having trouble finding protocols for your experiments? Check out our protocols section!
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<a href="https://2014.igem.org/Team:NTNU_Trondheim/Protocols" class="small button radius corner">More Info</a>
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Revision as of 01:48, 17 September 2015

Welcome to the NTNU Trondheim iGEM 2015 webpage!

NTNU Trondheim iGEM 2015

NTNU Trondheim is a team from Trondheim, Norway, competing for the iGEM 2015 student competition with glucose-sensing alginate-encapsulate Pseudomonas Putida. NTNU Trondheim maintains the iGEM Matchmaker Tool which helps iGEM teams cooperate with each other.


Project
We have constructed BioBricks that allow the insertion of transgenes into Synechocystis sp. PCC 6803. Working with photosynthetic organisms such as Synechocystis can help in the generation of environmental innovations.
We hope that our BioBricks can encourage and make it easier for future teams to work with Synechocystis and other photosynthetic organisms.



More Info

Judging criteria
See which judging criteria we have met.
More Info

Notebook
See our day to day activities in the lab.
More Info

Team
Meet the team behind NTNU's 2014 project.
More Info

Protocols
Having trouble finding protocols for your experiments? Check out our protocols section!
More Info