Difference between revisions of "Team:Penn/Safety"

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<p>We believe that our final project would not harbor any major safety issues to the research, public or the environment. The light-producing and light-sensing components of our project simply apply existing natural processes in a new setting.</p>
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<p><strong>What is your chassis organism?</strong></p>
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<p>We work with strains of <em>E.Coli</em>: NEB10, BL21, SY104 and HNS BW25113 Dhns::kan. We do not intend to plan experiments with any other organisms aside from our chassis.</p>
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<p><strong>What risks does your project pose at the laboratory stage? What actions are you taking to reduce those risks?</strong></p>
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<p>The strains of E.Coli that comprise our light-based communication system are highly conserved and pose minor risks when handled carefully. ATCC has assigned all strains a biosafety level of 1. In order to prevent contamination of any samples in the lab, including mammalian cell cultures, we dispose of all E. coli by bleaching cultures and then disposing of equipment in designated biohazard bins. All team members strictly adhere to the following safety procedures when performing experiments:&nbsp;</p>
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<li>All personnel wash their hands after they handle viable materials, after removing gloves, and before leaving the lab.&nbsp;</li>
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<li>Eating and drinking in the lab is not permitted. Food is stored outside the work area in cabinets or refrigerators.&nbsp;</li>
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<li>Policies for the safe handling of sharps are instituted.&nbsp;</li>
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<li>Work surfaces are decontaminated at least once a day and after any spill.&nbsp;</li>
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<li>All cultures, stocks and other wastes are decontaminated before disposal by an approved method such as autoclaving.&nbsp;</li>
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<li>Rubber gloves and protective eye-wear are worn at all times.</li>
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<p><strong>How would your project be used in the real world?</strong></p>
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<p>We are building a system of light-based communication between cell populations. This will allow for future research teams to take advantage of the orthogonality and spatiotemporal control of light as a medium for cell-to-cell communication. Additionally, we have shown that communication is possible across boundaries, so it is possible to promote talk between cells that grow in different environments or have different antibiotic resistances.</p>
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<p><strong>What risks might your project pose, if it were fully developed into a real product that real people could use? What future work might you do to reduce those risks?</strong></p>
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<tr class="tableizer-firstrow"><th>Species name (including strain)</th><th>Risk Group</th><th>Risk Group Source</th><th>Disease risk to humans?</th><th>Part number/name</th><th>Natural function of part</th><th>How did you acquire it?</th></tr>
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<p>We believe that the light-producing and light-sensing BioBricks do not raise any known safety issues to research, the public, or the environment. The system would most likely be utilized in a lab environment in order to promote communication across strains and environments. Therefore, general lab safety procedures must be followed in utilizing our communication system. Additionally, to guarantee public and environmental safety, researchers must properly dispose of bio-hazardous experimental waste to prevent undesirable spread of genes or toxicity into the environment.</p>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pACBB-eGFP</td><td>pACBB backbone with multiple cloning site; GFP is a naturally occurring protein made by the jellyfish Aequorea victoria.</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDAWN</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>BBa_K325909 pBAD + lux operon BioBrick on PSB1C3</td><td>Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. </td><td>Obtained from the BioBrick Registry</td></tr>
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<tr><td>E. coli NEB Turbo</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDawn + lacZ transformed into NEB turbo</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E.Coli BL21</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>BBa_K325909 pBAD + lux operon BioBrick on PSB1C3</td><td>Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. </td><td>Obtained from the BioBrick Registry</td></tr>
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<tr><td>E.Coli BL21</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDawn + lacZ transformed into BL21</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E.Coli BL21</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pACBB + BBa_K325909 lux operon ligated by Penn iGEM 2015 for testing if ligation successful by inducing pBAD in biobrick with arabinose</td><td>Vibrio fisheri, a bacterium that symbiotically lives in the light organ of certain squid species. The species produces a small autoinducer molecule (AHL) with expression of the LuxI gene. When the population density of the bacteria is high, the AHL molecule binds a transcriptional regulator, LuxR. The regulator then binds to the promoter pLux and activates the lux box, causing luminescence. </td><td>Insert obtained from the BioBrick Registry; Backbone obtained from the Chow lab at the Universtiy of Pennsylvania</td></tr>
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<tr><td>E.Coli DH5-alpha</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>uninduced  pDawn RFP</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>Obtained from the Chow lab at University of Pennsylvania</td></tr>
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<tr><td>E. coli NEB10</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pACBB + pBAD + lux operon & pDawn + mRFP</td><td>Cotransformed plasmids</td><td>Plasmid 1 obtained from the biobrick registry and Plasmid 2 obtained from the Chow lab at the University of Pennsylvania</td></tr>
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<tr><td>SY102</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>luxAB+sulA and lux CDE+ CP38 </td><td>Modified lux box. Split-lux" system in which gene expression of lux AB is under control of the sulA inducible promoter, but lux CDE expression is under a constitutive promoter (CP25/CP38).   </td><td>Grown up and innoculated from agar spear sample received from Dr. Shimshon Belkin at the Hebrew University, Jerusalem, Israel</td></tr>
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<tr><td>SY104</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>luxAB+sulA and luxCDE + CP25</td><td>Modified lux box. Split-lux" system in which gene expression of lux AB is under control of the sulA inducible promoter, but lux CDE expression is under a constitutive promoter (CP25/CP38).  </td><td>Grown up and innoculated from agar spear sample received from Dr. Shimshon Belkin at the Hebrew University, Jerusalem, Israel</td></tr>
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<tr><td>HNS BW25113 Dhns::kan strain</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pSB1C3 + pBAD + lux operon</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>pDawn + mRFP transformed into the HNS mutant strain obtained from Dr. Goulian at the University of Pennsylvania</td></tr>
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<tr><td>HNS BW25113 Dhns::kan strain</td><td>1</td><td>DSMZ </td><td>Strain derived from K-12. May be used in Level 1 laboratories with the most basic safety precautions, such as rubber gloves.</td><td>pDawn + mRFP</td><td>Blue-light photoreceptor for light-activated gene expression</td><td>pDawn + mRFP transformed into the HNS mutant strain obtained from Dr. Goulian at the University of Pennsylvania</td></tr>
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Revision as of 03:04, 17 September 2015

University of Pennsylvania iGEM

SAFETY

When the New York fashion community notices your brand, the world soon follows. The widespread love for UGG extended to Europe in the mid-2000's along with the stylish casual movement and demand for premium casual fashion. UGG boots and shoes were now seen walking the streets of London, Paris and Amsterdam with regularity. To meet the rising demand from new fans, UGG opened flagship stores in the UK and an additional location in Moscow. As the love spread farther East, concept stores were opened in Beijing, Shanghai and Tokyo. UGG Australia is now an international brand that is loved by all. This love is a result of a magical combination of the amazing functional benefits of sheepskin and the heightened emotional feeling you get when you slip them on your feet. In short, you just feel better all over when you wear UGG boots, slippers, and shoes.

In 2011, UGG will go back to its roots and focus on bringing the active men that brought the brand to life back with new styles allowing them to love the brand again as well. Partnering with Super Bowl champion and NFL MVP Tom Brady, UGG will invite even more men to feel the love the rest of the world knows so well. UGG will also step into the world of high fashion with UGG Collection. The UGG Collection fuses the timeless craft of Italian shoemaking with the reliable magic of sheepskin, bringing the luxurious feel of UGG to high end fashion. As the love for UGG continues to spread across the world, we have continued to offer new and unexpected ways to experience the brand. The UGG journey continues on and the love for UGG continues to spread.

We believe that our final project would not harbor any major safety issues to the research, public or the environment. The light-producing and light-sensing components of our project simply apply existing natural processes in a new setting.

What is your chassis organism?

We work with strains of E.Coli: NEB10, BL21, SY104 and HNS BW25113 Dhns::kan. We do not intend to plan experiments with any other organisms aside from our chassis.

What risks does your project pose at the laboratory stage? What actions are you taking to reduce those risks?

The strains of E.Coli that comprise our light-based communication system are highly conserved and pose minor risks when handled carefully. ATCC has assigned all strains a biosafety level of 1. In order to prevent contamination of any samples in the lab, including mammalian cell cultures, we dispose of all E. coli by bleaching cultures and then disposing of equipment in designated biohazard bins. All team members strictly adhere to the following safety procedures when performing experiments: 

  1. All personnel wash their hands after they handle viable materials, after removing gloves, and before leaving the lab. 
  2. Eating and drinking in the lab is not permitted. Food is stored outside the work area in cabinets or refrigerators. 
  3. Policies for the safe handling of sharps are instituted. 
  4. Work surfaces are decontaminated at least once a day and after any spill. 
  5. All cultures, stocks and other wastes are decontaminated before disposal by an approved method such as autoclaving. 
  6. Rubber gloves and protective eye-wear are worn at all times.

How would your project be used in the real world?

We are building a system of light-based communication between cell populations. This will allow for future research teams to take advantage of the orthogonality and spatiotemporal control of light as a medium for cell-to-cell communication. Additionally, we have shown that communication is possible across boundaries, so it is possible to promote talk between cells that grow in different environments or have different antibiotic resistances.

What risks might your project pose, if it were fully developed into a real product that real people could use? What future work might you do to reduce those risks?

We believe that the light-producing and light-sensing BioBricks do not raise any known safety issues to research, the public, or the environment. The system would most likely be utilized in a lab environment in order to promote communication across strains and environments. Therefore, general lab safety procedures must be followed in utilizing our communication system. Additionally, to guarantee public and environmental safety, researchers must properly dispose of bio-hazardous experimental waste to prevent undesirable spread of genes or toxicity into the environment.