Revision as of 04:25, 17 September 2015

BAC PACK

WETLAB COMPONENT

Description of above image

Goal

The goal of the wetlab component of BacPack is to connect the digital experience to a hands-on, lab-based one. In the digital activity visitors explore designing bacteria to have a variety of new, useful functions for helping humans survive in different extreme environments. In the wetlab activity visitors get try their hands at the same real-world process of engineering a bacteria to have a new property of their choosing.

Activity Design Outline

Visitors transform a living bacteria to make it do something new:
-5 DNA options that change the physical appearance of bacteria in different ways
-Each ‘color’ represents a bacteria function that can provide one of the 5 needs on Mars
-Visitors pick the DNA they want to use and transform e.coli with it
-They can see a photo of their grown ‘mars bacteria’ several days later

Development

Plasmids:
Selected 5 bioBricks from the iGEM distribution kit:
-Lux Operon (BBa_K325909) - Bioluminescence pathway
amilGFP (BBa_K592010) - Yellow chromoprotein
aeBlue (BBa_K864401) - Blue chromoprotein
eforRed (BBa_K592012) - Red/pink chromoprotein
asPink (BBa_K1033933) - Pink/purple chromoprotein

Transformation

Optimized to be accessible/easy for walk-up museum visitors of all ages and skill levels:

@@ Line 73: / Line 73: @@
 asPink (BBa_K1033933) - <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1033933” target="_blank" style="color:blue">Pink/purple chromoprotein</a><br>
 </p>
+<hr>
+<h3>Transformation</h3>
+<p>Optimized to be accessible/easy for walk-up museum visitors of all ages and skill levels:</p>
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