Difference between revisions of "Team:LZU-China/Parts"

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   <p>RibB is our basic gene. Every part basic on it.</p>
 
   <p>RibB is our basic gene. Every part basic on it.</p>
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   <p><img class="left-align" src="https://static.igem.org/mediawiki/parts/5/5f/Constitutive.png"> <img class="right-align" src="https://static.igem.org/mediawiki/parts/thumb/6/6e/IGEM_2015_MFC.JPG/700px-IGEM_2015_MFC.JPG"></p>
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   <p>
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    The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
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This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.
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Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
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This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
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                  <p>Figure 1:Constitutive parts </p>
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                  <img src="left-align" src="https://static.igem.org/mediawiki/parts/thumb/6/6e/IGEM_2015_MFC.JPG/600px-IGEM_2015_MFC.JPG">
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                </div>
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                  <p>Figure 2:Our parts working in MFC</p>
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      <P> We construct many parts as the following list.</P>
 
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Revision as of 08:09, 17 September 2015

Team:LZU 2015

 

Parts List

 

RibB is our basic gene. Every part basic on it.

The protein RibB catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate. This protein is involved in one step of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate. Proteins known to be involved in this subpathway in this organism are: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.

Figure 1:Constitutive parts

Figure 2:Our parts working in MFC

We construct many parts as the following list.

Parts list
Part ID Characterization Details
K1755001 ribB coding sequence more information
K1755002 ribB + B0014(terminator) more information
K1755003 B0032(ribosome binding sequence,RBS) + ribB + B0014(terminator) more information
K1755004 K525998(PT7+RBS) + ribB + B0014(terminator) more information
K1755005 K608003(strong promoter+medium RBS) + ribB + B0014(terminator) more information
K1755006 K608006(medium promoter+medium RBS) + ribB + B0014(terminator) more information
K1755007 B608002(strong promoter+strong RBS)+ ribB + B0014(terminator) more information
K1755008 K608004(strong Promoter and weak RBS)+ribB+B0014(Double terminator) more information
K1755009 K608007(Medium Promoter and weak RBS)+ribB+B0014(Double terminator) more information
K1755301 MarO(Copper sensor) + B0032(RBS) + ribB + B0014(terminator) more information
K1755302 K190024(Copper sensor) + ribB + B0014(terminator) more information
K1755303 K540001（cobalt sensor） + B0032(RBS) + ribB + B0014(terminator) more information
K1755305 K346002(MerO) + B0032(RBS) + ribB + B0014(terminator) more information
K1755024 K911003+ B0032(RBS) + ribB + B0014(terminator) more information