Difference between revisions of "Team:Bordeaux/AchievementsNotebook"

Protocols

Our Organisms of Choice is Escherichia coli and Saccharomyces cerevisiae . Listed below are the protocols we used in our lab.

Cloning

• PCR Methods iGEM Bordeaux
• E.coli Competent Cells
• Plasmid Extraction using Alkaline Lysis Method

Working with E.coli

• Growing Media of E.coli
• E.coli Curdlan Purification

Working with S.cerevisiae

• High Efficiency Yeast Transformation
• Genomic DNA Preparation from Yeast
• Gibson Assembly

Curdlan Methods

• Curdlan Quantification
• Sulfation of Curdlan by Sodium Pyrosulfate in Dimethyl Sulfoxide

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