Difference between revisions of "Team:CHINA CD UESTC/Results"
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<th scope="row">2</th> | <th scope="row">2</th> | ||
<td>piGEM-G6</td> | <td>piGEM-G6</td> | ||
− | <td><i>mamGFDC</i> + <i>mms6</i></td> | + | <td><i>mamGFDC</i>+<i>mms6</i></td> |
<td> | <td> | ||
Encode a series of proteins that can regulate the size and shape of crystals in the formation of magnetosome | Encode a series of proteins that can regulate the size and shape of crystals in the formation of magnetosome | ||
Line 409: | Line 409: | ||
<th scope="row">3</th> | <th scope="row">3</th> | ||
<td>piGEM-G6X</td> | <td>piGEM-G6X</td> | ||
− | <td><i>mamGFDC</i> + <i>mms6</i> + <i>mamXY</i></td> | + | <td><i>mamGFDC</i>+<i>mms6</i>+<i>mamXY</i></td> |
<td> | <td> | ||
Encode a series of proteins that can regulate the size and shape of crystals in the formation of magnetosome | Encode a series of proteins that can regulate the size and shape of crystals in the formation of magnetosome | ||
Line 420: | Line 420: | ||
<th scope="row">4</th> | <th scope="row">4</th> | ||
<td>piGEM-R-Lac</td> | <td>piGEM-R-Lac</td> | ||
− | <td><i>RFP</i> + <i>laccase</i></td> | + | <td><i>RFP</i>+<i>laccase</i></td> |
<td>Encode a fusion protein which makes Laccase visible</td> | <td>Encode a fusion protein which makes Laccase visible</td> | ||
<td><a href="http://parts.igem.org/Part:BBa_K1779204">BBa_K1779204</a></td> | <td><a href="http://parts.igem.org/Part:BBa_K1779204">BBa_K1779204</a></td> | ||
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<th scope="row">5</th> | <th scope="row">5</th> | ||
<td>piGEM-W-R-Lac</td> | <td>piGEM-W-R-Lac</td> | ||
− | <td><i>mamW</i> + <i>RFP</i> + <i>laccase</i></td> | + | <td><i>mamW</i>+<i>RFP</i>+<i>laccase</i></td> |
<td> | <td> | ||
Encode a fusion protein to bind Laccase to the transmembrane protein MamW, and RFP can make it visible | Encode a fusion protein to bind Laccase to the transmembrane protein MamW, and RFP can make it visible | ||
Line 440: | Line 440: | ||
<th scope="row">6</th> | <th scope="row">6</th> | ||
<td>piGEM-Plac-H</td> | <td>piGEM-Plac-H</td> | ||
− | <td><i>Lac I</i> promoter + <i>mamH</i></td> | + | <td><i>Lac I</i> promoter+<i>mamH</i></td> |
<td> | <td> | ||
To make sure whether <i>mamH</i> gene can successfully express | To make sure whether <i>mamH</i> gene can successfully express | ||
Line 449: | Line 449: | ||
<th scope="row">7</th> | <th scope="row">7</th> | ||
<td>piGEM-Plac-G</td> | <td>piGEM-Plac-G</td> | ||
− | <td><i>Lac I</i> promoter + <i>mamG</i></td> | + | <td><i>Lac I</i> promoter+<i>mamG</i></td> |
<td>To make sure whether <i>mamG</i> gene can successfully express</td> | <td>To make sure whether <i>mamG</i> gene can successfully express</td> | ||
<td></td> | <td></td> | ||
Line 457: | Line 457: | ||
<th scope="row">8</th> | <th scope="row">8</th> | ||
<td>piGEM-Plac-6</td> | <td>piGEM-Plac-6</td> | ||
− | <td><i>Lac I</i> promoter + <i>mms6</i></td> | + | <td><i>Lac I</i> promoter+<i>mms6</i></td> |
<td>To make sure whether <i>mamH</i> gene can successfully express</td> | <td>To make sure whether <i>mamH</i> gene can successfully express</td> | ||
<td></td> | <td></td> | ||
Line 465: | Line 465: | ||
<th scope="row">9</th> | <th scope="row">9</th> | ||
<td>piGEM-Plac-Y</td> | <td>piGEM-Plac-Y</td> | ||
− | <td><i>Lac I</i> promoter + <i>mamY</i></td> | + | <td><i>Lac I</i> promoter+<i>mamY</i></td> |
<td>To make sure whether <i>mamH</i> gene can successfully express</td> | <td>To make sure whether <i>mamH</i> gene can successfully express</td> | ||
<td></td> | <td></td> | ||
Line 473: | Line 473: | ||
<th scope="row">10</th> | <th scope="row">10</th> | ||
<td>piGEM-PH-R-1</td> | <td>piGEM-PH-R-1</td> | ||
− | <td><i>mamH</i> promoter + <i>RFP</i></td> | + | <td><i>mamH</i> promoter+<i>RFP</i></td> |
<td>To make sure whether <i>mamH</i> promoter can work</td> | <td>To make sure whether <i>mamH</i> promoter can work</td> | ||
<td></td> | <td></td> | ||
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<th scope="row">11</th> | <th scope="row">11</th> | ||
<td>piGEM-PG-R</td> | <td>piGEM-PG-R</td> | ||
− | <td><i>mamG</i> promoter + <i>RFP</i></td> | + | <td><i>mamG</i> promoter+<i>RFP</i></td> |
<td>To make sure whether <i>mamG</i> promoter can work</td> | <td>To make sure whether <i>mamG</i> promoter can work</td> | ||
<td></td> | <td></td> | ||
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<th scope="row">12</th> | <th scope="row">12</th> | ||
<td>piGEM-P6-R</td> | <td>piGEM-P6-R</td> | ||
− | <td><i>mms6</i> promoter + <i>RFP</i></td> | + | <td><i>mms6</i> promoter+<i>RFP</i></td> |
<td>To make sure whether <i>mms6</i> promoter can work</td> | <td>To make sure whether <i>mms6</i> promoter can work</td> | ||
<td></td> | <td></td> | ||
Line 497: | Line 497: | ||
<th scope="row">13</th> | <th scope="row">13</th> | ||
<td>piGEM-PY-R</td> | <td>piGEM-PY-R</td> | ||
− | <td><i>mamY</i> promoter + <i>RFP</i></td> | + | <td><i>mamY</i> promoter+<i>RFP</i></td> |
<td>To make sure whether <i>mamY</i> promoter can work</td> | <td>To make sure whether <i>mamY</i> promoter can work</td> | ||
<td></td> | <td></td> | ||
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<th scope="row">14</th> | <th scope="row">14</th> | ||
<td>piGEM-PH-R-2</td> | <td>piGEM-PH-R-2</td> | ||
− | <td><i>mamH</i> promoter + <i>RFP</i></td> | + | <td><i>mamH</i> promoter+<i>RFP</i></td> |
<td>To make sure whether <i>mamH</i> promoter can work and verify the backbone of pET28a</td> | <td>To make sure whether <i>mamH</i> promoter can work and verify the backbone of pET28a</td> | ||
<td></td> | <td></td> | ||
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<th scope="row">15</th> | <th scope="row">15</th> | ||
<td>piGEM-LacI-RFP</td> | <td>piGEM-LacI-RFP</td> | ||
− | <td><i>Lac I</i> promoter + <i>RFP</i></td> | + | <td><i>Lac I</i> promoter+<i>RFP</i></td> |
<td>To verify the backbone of pET28a</td> | <td>To verify the backbone of pET28a</td> | ||
<td></td> | <td></td> | ||
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<th scope="row">16</th> | <th scope="row">16</th> | ||
<td>piGEM-GFP-PG6-R</td> | <td>piGEM-GFP-PG6-R</td> | ||
− | <td><i>GFP<i> + <i>mamG-mms6</i> promoter + <i>RFP</i></td> | + | <td><i>GFP<i>+<i>mamG-mms6</i> promoter+<i>RFP</i></td> |
<td>To make sure whether <i>mamG-mms6</i> promoter can work</td> | <td>To make sure whether <i>mamG-mms6</i> promoter can work</td> | ||
<td></td> | <td></td> |
Revision as of 08:18, 17 September 2015
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RESULTS
Fortunately, we got the fusion proteins MamW + RFP + Laccase and RFP + Laccase. The fusion protein RFP + Laccase worked very well in our EBFC. What's more, after we co-transferred the two vectors piGEM-AB and piGEM-G6X into Escherichia coli BL21(DE3), there were something interesting appeared in the bacteria!