Difference between revisions of "Team:Scut-Champion-Park/Description"

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<title>iGEM - Champion Park</title>
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<link rel="stylesheet" type="text/css" href="https://2015.igem.org/Team:Scut-Champion-Park/css/index.css/?action=raw&ctype=text/css">
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<h2> Project Description </h2>
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<div class="container">
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<div class="header">
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<!-- <img src="./resources/igem_header_image.png" width="100%" height="100%"/> -->
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<div class="banner">
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<div class="clear"> </div>
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<div class="menu">
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<div class="menu-bar">
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<table class="menu-table">
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<td style="width:63px">
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<span class="menu-tag" href="#"> HOME </span>
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</ul>
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</td>
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<td style="width:92px">
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<span class="menu-tag"> PROJECT </span>
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<ul class="menu-list" >
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<li><a href="/Team:Scut-Champion-Park/Project/Overview" class="alink"> Over View </a></li>
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<li><a href="/Team:Scut-Champion-Park/Project/Protocols" class="alink"> Protocols </a></li>
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<li><a href="/Team:Scut-Champion-Park/Design" class="alink"> Design </a></li>
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<td style="width:125px">
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<span class="menu-tag"> ACHIEVEMENT </span>
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<ul class="menu-list">
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<li><a href="/Team:Scut-Champion-Park/Achievement/Results" class="alink"> Results </a></li>
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<li><a href="/Team:Scut-Champion-Park/Basic_Part" class="alink">  Registry Part </a></li>
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                                                                                                <li><a href="/Team:Scut-Champion-Park/Description" class="alink"> Contribution </a></li>
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<li><a href="/Team:Scut-Champion-Park/Achievement/Judging_Form" class="alink"> Judging Form </a></li>
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<li><a href="/Team:Scut-Champion-Park/Achievement/Financing" class="alink"> Financing </a></li>
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<li><a href="/Team:Scut-Champion-Park/Achievement/Acknowledgement" class="alink"> Acknowledgement </a></li>
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<span class="menu-tag"> POCLICY &amp; PRACTICES </span>
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<ul class="menu-list">
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<li><a href="/Team:Scut-Champion-Park/Practices" class="alink"> Over View </a></li>
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<li><a href="/Team:Scut-Champion-Park/Practices/Policy" class="alink"> Policy &amp; Solution </a></li>
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<li><a href="/Team:Scut-Champion-Park/Practices/Research" class="alink"> Research &amp; Solution </a></li>
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<li><a href="/Team:Scut-Champion-Park/Practices/Meet_Ups" class="alink"> Meet Ups </a></li>
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                                                                                                <li><a href="/Team:Scut-Champion-Park/Collaborations" class="alink"> Collaborations </a></li>
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<li><a href="/Team:Scut-Champion-Park/Practices/Social_Media " class="alink"> Social Media </a></li>
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<li><a href="/Team:Scut-Champion-Park/Entrepreneurship" class="alink"> Entrepreneurship </a></li>
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<li><a href="/Team:Scut-Champion-Park/Practices/Super_Brochure " class="alink"> Super Brochure </a></li>
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</ul>
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</td>
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<td style="width:118px">
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<span class="menu-tag"> TEAM </span>
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<ul class="menu-list">
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<li><a href="/Team:Scut-Champion-Park/Team/Team_Member" class="alink"> Team Member </a></li>
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<li><a href="/Team:Scut-Champion-Park/Attributions" class="alink"> Attributions </a></li>
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<li><a href="/Team:Scut-Champion-Park/Team/Team_Identity" class="alink"> Team Identity </a></li>
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<li><a href="/Team:Scut-Champion-Park/Team/Contact_Us" class="alink"> Contact Us </a></li>
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<li><a href="https://igem.org/Team.cgi?id=1778" class="alink"> Offical Team Profile </a></li>
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</ul>
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</td>
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<td style="width:90px">
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<span class="menu-tag"> SAFETY </span>
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<ul class="menu-list">
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<li><a href="/Team:Scut-Champion-Park/Safety" class="alink"> Safety </a></li>
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</ul>
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</td>
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</tr>
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</tbody>
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</table>
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</div>
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<div class="igem_logo"></div>
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<div class="team_logo"></div>
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<div class="clear"> </div>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
 
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<h5>What should this page contain?</h5>
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<div class="main-container">
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<ul>
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<div class="contrib-container">
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<li> A clear and concise description of your project.</li>
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<div class="contrib-wrapper">
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<div class="contrib-content-container contrib-content-container-1 bg-radius bg-shade">
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<li>References and sources to document your research.</li>
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<div class="contrib-content">
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h1 class="contrib-title"> Contribution </h1>
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<p class="contrib-main-content">
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Our team improved the function and characterization of previously existing BioBrick Parts (created by one of our univerisity teams <span style="color:#02b2da;">SCUT</span> in 2014 of iGEM), and entered this information in the part's page on the Registry. Please see the Registry Contribution below, and you can click on the name of parts to see more detailed information. (These parts do not come from our team's 2015 range of part numbers)
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</p>
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<p class="contrib-main-content">
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This experiment is designed to demonstrate the improvement of element's function. We use the fluorescence quantitative PCR method, add a pair of primers and a special fluorescent probe which is oligonucleotides with report fluorescent groups and fluorescence quenching groups at the ends. When the probe is complete, the fluorescence signal emitted by report fluorescent groups are absorbed by fluorescence quenching groups. When the PCR begin, the probe combine with a single chain of DNA. Then in the amplification, Taq enzyme, a the 5'-3'end excision enzyme degrade the probe, make the report fluorescence groups and the fluorescence quenching groups separate. Then the fluorescence monitoring system can detect the fluorescence signal. One DNA chain, one fluorescent molecular. We can identify the yield of PCR by detecting the fluorescence signal. We also draw a standard curve to quantify the experimental result.
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<th> Name </th>
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<th> Type </th>
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<th> Description </th>
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<th> Designer </th>
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<th> Length </th>
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</tr>
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<tr>
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<td> <span style="color:#02b2da; font-weight:bold">BBa_K1462430</span> </td>
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<td> Composite </td>
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<td> pTEF2+GFP+tADH1 </td>
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<td> Haonan Qi </td>
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<td> 1486 </td>
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</tr>
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<tr>
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<td> <span style="color:#02b2da; font-weight:bold">BBa_K1462440</span> </td>
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<td> Composite </td>
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<td> pTDH3+GFP+tADH1 </td>
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<td> Haonan Qi </td>
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<td> 1583 </td>
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</tr>
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<tr>
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<td> <span style="color:#02b2da; font-weight:bold">BBa_K1462450</span> </td>
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<td> Composite </td>
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<td> pGAL1+GFP+tADH1 </td>
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<td> Haonan Qi </td>
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<td> 1632 </td>
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</tr>
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</table>
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</div>
  
  
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<div class="footer">
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<h4>Advice on writing your Project Description</h4>
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<div class="team_info">
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Team Email: scut-champion-park@hotmail.com <br/><br/>
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<p>
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Address: School of Bioscience &amp; Bioengineering <br/>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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South China University of Technology, Building B6 Guangzhou<br/>
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</p>
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Higher Education Mega Centre, Panyu District,<br/>
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Guangzhou, China <br/>
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<p>
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</div>
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h4>References</h4>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you though about your project and what works inspired you.</p>
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<h4>Inspiration</h4>
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<p>See how other teams have described and presented their projects: </p>
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<ul>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Revision as of 08:52, 17 September 2015

iGEM - Champion Park

Contribution

Our team improved the function and characterization of previously existing BioBrick Parts (created by one of our univerisity teams SCUT in 2014 of iGEM), and entered this information in the part's page on the Registry. Please see the Registry Contribution below, and you can click on the name of parts to see more detailed information. (These parts do not come from our team's 2015 range of part numbers)

This experiment is designed to demonstrate the improvement of element's function. We use the fluorescence quantitative PCR method, add a pair of primers and a special fluorescent probe which is oligonucleotides with report fluorescent groups and fluorescence quenching groups at the ends. When the probe is complete, the fluorescence signal emitted by report fluorescent groups are absorbed by fluorescence quenching groups. When the PCR begin, the probe combine with a single chain of DNA. Then in the amplification, Taq enzyme, a the 5'-3'end excision enzyme degrade the probe, make the report fluorescence groups and the fluorescence quenching groups separate. Then the fluorescence monitoring system can detect the fluorescence signal. One DNA chain, one fluorescent molecular. We can identify the yield of PCR by detecting the fluorescence signal. We also draw a standard curve to quantify the experimental result.

Name Type Description Designer Length
BBa_K1462430 Composite pTEF2+GFP+tADH1 Haonan Qi 1486
BBa_K1462440 Composite pTDH3+GFP+tADH1 Haonan Qi 1583
BBa_K1462450 Composite pGAL1+GFP+tADH1 Haonan Qi 1632
Team Email: scut-champion-park@hotmail.com

Address: School of Bioscience & Bioengineering
South China University of Technology, Building B6 Guangzhou
Higher Education Mega Centre, Panyu District,
Guangzhou, China