Difference between revisions of "Team:CGU Taiwan/Notebook"

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                            <h1 class="title">Blog</h1>
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                            <p>Timeline</p>
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                        <a href="#" class="btn btn-common uppercase">August 2015</a>
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<tr><td><a>Description</a></td><td><a>Yeast With IL-8 Receptor</a></td><td><a>Toehold Switch As RNA Senor</a></td><td><a>Prototype Design</a></td></tr>
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                                        <img src="https://static.igem.org/mediawiki/2015/8/82/CGU%26Missouri_University_Pro._David_Westenberg-850x400.jpg" class="img-responsive" alt="">
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                                          <span class="uppercase"><a href="#">05 <br><small>Agu</small></a></span>
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<h2 class="post-title bold"><a href="blogdetails.html">Experience sharing with Prof. Westenberg from Missouri University iGEM team</a></h2>
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<h3 class="post-author"><a href="#">Posted by micron News</a></h3>
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<p>Pro. David from Missouri University came to CGU today!<br>We had a really great time sharing each other's project, and show him around our campus~<br>Thank you so much for the experiences sharing~</p>
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                                          <span class="uppercase"><a href="#">19 <br><small>Jul</small></a></span>
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<h3 class="post-author"><a href="#">----------</a></h3>
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<p>-----------<br>--------------------------------------------<br>------------<br>----------------<br></p>
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Operator: Wan yun, Jinting , Jinye
 +
Goal:
 +
1. Extraction of gDNA of Far1∆::KANMX strain
 +
    2. Detection the concentration of fast extracted gDNA of ∆Far1 strain
 +
3. PCR gDNA of Far1∆ ::KANMX
 +
4. Electrophoresis to check PCR product
 +
Experiment steps:
 +
< Extraction of gDNA of Far1∆::KANMX strain>
 +
Consult the protocol<protocol of fast extraction of gDNA of yeast>
  
 +
<Detection the concentration fo fast extracted gDNA>
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<table class="protocol-table">
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<tr><td>Strains </td><td>260/280 </td><td>260/230 </td><td>C(ng/μl) </td></tr>
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<tr><td>Far1∆::KANMX strain </td><td>1.62 </td><td>0.97 </td><td>44.7 </td></tr>
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</table>
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<PCR gDNA of Far1∆::KANMX >
 +
1. Design of primers
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<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>55</td><td>21.25</td><td>212.50</td></tr>
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<tr><td> </td><td> </td><td> </td><td> </td><td> </td><td> </td><td>41</td><td>17.12</td><td>171.20</td></tr>
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Home | CGU_Taiwan

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DescriptionYeast With IL-8 ReceptorToehold Switch As RNA SenorPrototype Design

GPCR

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol
Strains 260/280 260/230 C(ng/μl)
Far1∆::KANMX strain 1.62 0.97 44.7
1. Design of primers
Tm (℃)GC (%)Nmoleμl for 100μM
5521.25212.50
4117.12171.20