Difference between revisions of "Team:Bordeaux/Yeast results"
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<h6 align="justify"><i>FKS1</i> gene</h6 | <h6 align="justify"><i>FKS1</i> gene</h6 | ||
<p align=justify> <i>FKS1</i> gene codes a D-glucan synthase. </p> | <p align=justify> <i>FKS1</i> gene codes a D-glucan synthase. </p> | ||
| − | + | ||
<h6 align="justify"><i>pYES2</i> plasmid</h6> | <h6 align="justify"><i>pYES2</i> plasmid</h6> | ||
<p align="justify">The plasmid used is the <i>pYES2</i> plasmid provided by <b>M. Didier Thoraval</b> (membrane biogenesis laboratory). It contains an inducible promoter to galactose (<i>GAL1</i>) and a selection gene <i>URA3</i>.</p> | <p align="justify">The plasmid used is the <i>pYES2</i> plasmid provided by <b>M. Didier Thoraval</b> (membrane biogenesis laboratory). It contains an inducible promoter to galactose (<i>GAL1</i>) and a selection gene <i>URA3</i>.</p> | ||
| + | |||
<h6 align="justify"><i>Saccharomyces cerevisiae</i> strain</h6> | <h6 align="justify"><i>Saccharomyces cerevisiae</i> strain</h6> | ||
| − | <p align="justify"> All results were obtained with haploid Lnvsc1 strain of Saccharomyces cerevisiae. Its genotype | + | <p align="justify"> All results were obtained with haploid <b>Lnvsc1 strain</b> of Saccharomyces cerevisiae. Its genotype is Leu / Trp / Ura- / Hist- mat α. |
This genotype allows us an easy selection of transformed cells. </p> | This genotype allows us an easy selection of transformed cells. </p> | ||
Revision as of 12:13, 17 September 2015