Difference between revisions of "Team:Warwick/Parts"
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+ | Our new parts are all versions of the same modular system, a fusion protein formed from a bacterial transmembrane domain and a zinc-finger domain, with linker domains between domains and a FLAG-tag for immunofluorescence staining. Each transmembrane/zinc-finger domain is independently interchangeable with other transmembrane/zinc-finger domains, though not interchangeable between each other (i.e. the transmembrane domain cannot be swapped for a zinc-finger domain and vice versa). | ||
+ | <br> The purpose of this fusion protein is to allow bacteria (specifically Escherichia coli) to bind specific short double stranded DNA sequences at their surface; using immobilised DNA the cell should remain bound in place, or this protein can be used for cell identification (barcoding). | ||
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+ | <h5 class="sidebartitle">Lpp-OmpA-Zif268</h5> | ||
<p> | <p> | ||
− | + | This part is our construct with Lpp-OmpA as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | |
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− | <h5 class="sidebartitle"> | + | <h5 class="sidebartitle">Lpp-OmpA-sZF2</h5> |
<p> | <p> | ||
− | + | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF2 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | |
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− | <h5 class="sidebartitle"> | + | <h5 class="sidebartitle">Lpp-OmpA-sZF10</h5> |
<p> | <p> | ||
− | + | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF10 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | |
</p> | </p> | ||
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+ | <h5 class="sidebartitle">Lpp-OmpA-sZF14</h5> | ||
+ | <p> | ||
+ | This part is our construct with Lpp-OmpA as the transmembrane domain and sZF14 as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
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+ | </p> | ||
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+ | <h5 class="sidebartitle">INP-Zif268</h5> | ||
+ | <p> | ||
+ | This part is our construct with INP as the transmembrane domain and the Zif268 1AAY fragment as the zinc-finger domain. The transmembrane domain can be exchanged with another using NdeI and AgeI, and the zinc-finger domain can be exchanged using NheI and ClaI. The entire construct is under the pL_lac promoter, and contains a C-terminal FLAG tag. | ||
+ | </p> | ||
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<h5 class="sidebartitle">Part 4</h5> | <h5 class="sidebartitle">Part 4</h5> | ||
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Revision as of 12:14, 17 September 2015