Difference between revisions of "Team:Bordeaux/Meetup"
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<p align="justify" style="text-indent: 3vw;"> This year, Bordeaux team hosted the <b>French Meet-up</b> with the help of Cap Sciences (Scientific Museum in Bordeaux specialized in scientific popularization) during the last weekend of August (29-30th). We invited all French and francophone teams. Aix-Marseille University iGEM team, Paris_Saclay iGEM team, Pasteur_Paris iGEM team and Toulouse iGEM team came from France, KU_Leuven iGEM team came from Belgium and EPF_Lausanne iGEM team came from Switzerland and all enjoyed the weekend with us. </p> | <p align="justify" style="text-indent: 3vw;"> This year, Bordeaux team hosted the <b>French Meet-up</b> with the help of Cap Sciences (Scientific Museum in Bordeaux specialized in scientific popularization) during the last weekend of August (29-30th). We invited all French and francophone teams. Aix-Marseille University iGEM team, Paris_Saclay iGEM team, Pasteur_Paris iGEM team and Toulouse iGEM team came from France, KU_Leuven iGEM team came from Belgium and EPF_Lausanne iGEM team came from Switzerland and all enjoyed the weekend with us. </p> | ||
− | <p align="justify" style="text-indent: 3vw;"> On Saturday morning, iGEM Bordeaux organized an <b>orientation races</b> in the city. The objective was to answer enigmas about synthetic biology and other iGEM projects to find clues for the next location they needed to go to. Divided into three teams, we were able to visit the beautiful city of Bordeaux which ended in a picnic at the Cap Science Museum. </p> | + | <p align="justify" style="text-indent: 3vw;"> On Saturday morning, iGEM Bordeaux organized an <b>orientation races</b> in the city. The objective was to answer enigmas about synthetic biology and other iGEM projects to find clues for the next location they needed to go to. Divided into three teams, we were able to visit the beautiful city of Bordeaux which ended in a picnic at the Cap Science Museum. </p> |
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<p align="justify" style="text-indent: 3vw;"> There, teams presented <b>their projects</b> in front of other teams and general public at Cap Sciences and we talked with the audience about the inconvenient and advantages of each project. Each team had to make their presentation understandable for everyone. We also were lucky to have a <b>theatre expert</b> who gave us <b>constructive feedback</b> on the best way to <b>present our projects</b> at Boston and to a non-scientific public. </br> </br> | <p align="justify" style="text-indent: 3vw;"> There, teams presented <b>their projects</b> in front of other teams and general public at Cap Sciences and we talked with the audience about the inconvenient and advantages of each project. Each team had to make their presentation understandable for everyone. We also were lucky to have a <b>theatre expert</b> who gave us <b>constructive feedback</b> on the best way to <b>present our projects</b> at Boston and to a non-scientific public. </br> </br> | ||
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<p align="justify" style="text-indent: 3vw;"> On Sunday, we all met at the Jardin Public (Bordeaux Park) to do some activities proposed by <b>iGEM Paris_Saclay and iGEM Pasteur_Paris</b> teams. The first one organized a <b>debate about biosafety</b>, and filmed a short video of iGEM team presentation on biosafety for each project. This encouraged all teams to think about <b>safety considerations</b> in their project and allowed us to discuss general safety issues such as containing <b>Genetically Modified Organisms</b> and ensuring that they do not become out of our control.</br> | <p align="justify" style="text-indent: 3vw;"> On Sunday, we all met at the Jardin Public (Bordeaux Park) to do some activities proposed by <b>iGEM Paris_Saclay and iGEM Pasteur_Paris</b> teams. The first one organized a <b>debate about biosafety</b>, and filmed a short video of iGEM team presentation on biosafety for each project. This encouraged all teams to think about <b>safety considerations</b> in their project and allowed us to discuss general safety issues such as containing <b>Genetically Modified Organisms</b> and ensuring that they do not become out of our control.</br> | ||
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<center> <FONT COLOR="red"> You can see the little videos which have been made this day following this link: LINK.</FONT></center> </br> </br> | <center> <FONT COLOR="red"> You can see the little videos which have been made this day following this link: LINK.</FONT></center> </br> </br> | ||
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<br> They decided to test curdlan toxicity on two Bacteria, <I>Rhodobacter sphaeroides</I> and <I>Pseudomonas putida</I>. They choose these bacteria because these two species are very common in the environment so they believe these bacteria represent a good marker for an eventual environmental toxicity.</p> | <br> They decided to test curdlan toxicity on two Bacteria, <I>Rhodobacter sphaeroides</I> and <I>Pseudomonas putida</I>. They choose these bacteria because these two species are very common in the environment so they believe these bacteria represent a good marker for an eventual environmental toxicity.</p> | ||
− | <p><b>Methods and Materials</b></p> | + | <p align ="left" ><b>Methods and Materials</b></p> |
<p align="justify" style="text-indent: 3vw;">Single colonies of wild-type <I>Rhodobacter sphaeroides</I> WS8N and <I>Pseudomonas putida </I> KT2440 were grown in SUX media and Lysogeny Broth respectively overnight to stationary | <p align="justify" style="text-indent: 3vw;">Single colonies of wild-type <I>Rhodobacter sphaeroides</I> WS8N and <I>Pseudomonas putida </I> KT2440 were grown in SUX media and Lysogeny Broth respectively overnight to stationary | ||
phase at 30°C with orbital shaking at 225 rpm. 1% w/v curdlan in NaOH was made by adding 0.1g of solid curdlan to 10mL of 0.5M NaOH and warming the mixture at 55°C with regular vigorous shaking for 15 minutes to achieve complete dissolution. 100µL of stationary culture was added to 100µL of curdlan-NaOH or NaOH in the wells of a Corning 96-well flat bottom plate, and each condition was repeated in six-plicate. The plate was incubated at 30°C in a FLUOstar Omega Microplate Reader with 200 rpm shaking in between reads, and OD600 was monitored every 15 minutes. | phase at 30°C with orbital shaking at 225 rpm. 1% w/v curdlan in NaOH was made by adding 0.1g of solid curdlan to 10mL of 0.5M NaOH and warming the mixture at 55°C with regular vigorous shaking for 15 minutes to achieve complete dissolution. 100µL of stationary culture was added to 100µL of curdlan-NaOH or NaOH in the wells of a Corning 96-well flat bottom plate, and each condition was repeated in six-plicate. The plate was incubated at 30°C in a FLUOstar Omega Microplate Reader with 200 rpm shaking in between reads, and OD600 was monitored every 15 minutes. |
Revision as of 13:40, 17 September 2015