Difference between revisions of "Team:KU Leuven/Notebook/Newsfeed"

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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
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<p>-We are in the track ‘New applications’<br/>
+
<p>- We are in the track ‘New applications’<br/>
−
-Received offers for hoodies, stickers and tattoos <br/>
+
- Receiving offers for hoodies, stickers and tattoos <br/>
−
-Collaboration: Skype session with TU Delft <br/>
+
- Collaboration: Skype session with TU Delft <br/>
−
-Collaboration: Measure pH of tap water and river water & sending samples to the iGEM team of York <br/>
+
- Collaboration: Measuring pH of tap water and river water & sending samples to the iGEM team of York <br/>
−
-Collaboration: Interviewed people on the street for chewing-gum survey of the iGEM team of Aix-Marseille Université <br/>
+
- Collaboration: Interviewing people on the street for chewing-gum survey of the iGEM team of Aix-Marseille Université <br/>
−
-We translated our survey in French for further distribution in Wallonie.
+
- Translating our survey in French for further distribution in Wallonie.
 
</p>
 
</p>
 
     </div>
 
     </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-We searched more information about lab safety. <br/>
+
<p>- Searching more information about lab safety <br/>
−
-We utilized literature to prepare our experiment of the InterLab Measurement Study.</p>
+
- Analyzing the results of the InterLab Measurement Study</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-This week we made electrocompetent cells. Their transformation efficiency is higher than that of chemocompetent cells, so they could be useful in making BioBricks and transforming our assembled gBlocks.<br/>
+
<p>- Preparing electrocompetent cells<br/>
−
-We repeated the tail PCR of luxR. This time we performed a touchdown PCR in order to become more specific results. <br/>
+
- Optimizing the tail PCR to make Biobricks <br/>
−
-Our three BioBricks were purified by using a gel extraction kit. After this, we inserted our BioBricks in pSB1C3. The next step is screening colonies by PCR to find plasmids with the right insert.<br/>
+
- Cloning the PCR products into pSB1C3, transforming the plasmids and screening of colonies to find the correct insert <br/>
−
-We made our devices for the InterLab Measurement Study by using the BioBrick Assembly Method. After this we transformed electrocompetent <i>E. cloni</i> with our devices. First we grew our cells under the recommended conditions of iGEM on plates and afterwards we made a liquid culture. On Friday, we made our standard curve based on fluorescein and we measured our devices.
+
- Transforming the devices for the Interlab Measurement Study <br/>
−
<br/>
+
- Preparation of the standard curve based on fluorescein and measuring the fluorescence of the new devices <br/>
−
-We used the NEBuilder® HiFi DNA Assembly Master Mix to assemble our gBlocks. After doing a PCR, we visualised our results on a gel. We obtained a positive result for gBlocks 5 and 6. So we transformed these assembled gBlocks in electrocompetent <i>E. cloni</i>. We checked our  transformed gBlocks on PCR, but a confirmation by restriction mapping is still needed.
+
- Assembling the gBlocks using NEBuilder® HiFi DNA Assembly Master Mix and transforming the checked pcr products in electrocompetent <i>E. cloni</i> </p>
−
</p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Meeting with Tim Odenthal <br/>
+
<p>- Meeting with Tim Odenthal <br/>
−
-Optimization of hybrid model code <br/>
+
- Optimization of hybrid model code <br/>
−
-Implementation of cell-cell interactions, including repulsion as well as attraction
+
- Implementation of cell-cell interactions, including repulsion as well as attraction</p>
−
</p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Update Facebook & Twitter <br/>
+
<p>- Updating Facebook & Twitter <br/>
−
-Spreading the survey on social media & inform people about the symposium <br/>
+
- Spreading the survey on social media & informing people about the symposium <br/>
−
-Appointment with Ilse Frederickx for KU Leuven press </p>
+
- Appointment with Ilse Frederickx for KU Leuven press </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Contacting speakers for symposium<br/>
+
<p>- Contacting keynote speakers for the Symposium<br/>
−
-Organizing symposium <br/>
+
- Organizing symposium <br/>
−
-Mailing schools to give a playful course about synthetic biology <br/>
+
- Mailing schools to give a playful course about synthetic biology <br/>
−
-We decided on rules for a nice card game about synthetic biology </p>
+
- Deciding on the rules for a nice card game about synthetic biology </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Adapting pages <br/>
+
<p>- Adapting pages <br/>
−
-Putting ‘Symposium’ page online</p>
+
- Putting ‘Symposium’-page online</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Making informative images for the Research-page <br/>
+
<p>- Making informative images for the Research-page <br/>
−
-Making buttons for the wiki <br/>
+
- Making buttons for the wiki <br/>
−
-Design bacteria-stickers for use in schools and as gadget <br/>
+
- Designing bacteria-stickers for use in schools and as gadgets <br/>
−
-Continue with the design of our hoodies <br/>
+
- Continuing with the design of our hoodies <br/>
−
-Making funny images for our secret page on our wiki </p>
+
- Designing funny images for our secret page on the wiki </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Our flyers and sponsor brochures arrived!<br/>
+
<p>- Our flyers and sponsor brochures arrived!<br/>
−
-Survey about synthetic biology with people in the street <br/>
+
- Conducting a survey about synthetic biology interviewing people on the street <br/>
−
-Working on a team song
+
- Working on a team song
 
</p>
 
</p>
 
     </div>
 
     </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Making a protocol for leucine detection <br/>
+
<p>- Making a protocol for leucine detection <br/>
−
-Research about lab safety</p>
+
- Researching lab safety</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-The transformation of chemocompetent cells for the Interlab Measurement Study was not efficient, therefore we repeat this with electrocompetent cells. We grew the transformed cells overnight and performed a miniprep.<br/>
+
<p>- Switching from transformation of chemocompetent cells to electroporation due to low efficiency for the Interlab Measurement Study <br/>
−
-To check if the operon of Δ<i>tar</i>Δ<i>cheZ</i> is OK, we used a PCR to confirm that all the genes are present.<br/>
+
- Checking the intactness of other genes in the tar-tap-cheRBYZ operon by PCR <br/>
−
-After performing a PCR, there were no bands visible of the assembled gBlocks, probably we didn’t use enough Gibson Assembly Master Mix <br/>
+
- Ordering NEBuilder to repeat the failed Gibson Assembly <br/>
−
-We started making three BioBricks starting from our gBlocks. We performed a high fidelity tail PCR to include the prefix and suffix in our BioBrick. The parts were digested and purified on a gel. <br/>
+
- Making three BioBricks starting from our gBlocks by tail PCR and restriction digestion <br/>
−
-We ordered materials for leucine and AHL detection </p>
+
- Ordering materials for leucine and AHL detection </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Finish implementing hybrid model I in 2D, including ADI scheme for PDE part <br/>
+
<p>- Finishing implementation of the hybrid model I in 2D, including ADI scheme for PDE part <br/>
−
-Extend hybrid model II to 2D <br/>
+
- Extending hybrid model II to 2D <br/>
−
-Run hybrid model simulations <br/>
+
- Running hybrid model simulations <br/>
−
-Finishing report Simbiology <br/>
+
- Finishing report Simbiology <br/>
−
-Contacting Toulouse team for collaboration <br/>
+
- Contacting Toulouse team for collaboration <br/>
−
-Contacting professors for possible collaboration</p>
+
- Contacting professors for possible collaboration</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Update Facebook & Twitter </p>
+
<p>- Updating Facebook & Twitter </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Thinking of educational games <br/>
+
<p>- Brainstorming about educational games </p>
−
-Outreach: Put popular message about our project on Facebook and Twitter </p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Our ‘Newsfeed’ & ‘Research’-page are online! <br/>
+
<p>- Putting our ‘Newsfeed’ & ‘Research’-page online! <br/>
−
-Implemented Lightbox and EasySwitch button</p>
+
- Implementing an EasySwitch button and a Lightbox</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Making informative images for the ‘Research’-page <br/>
+
<p>- Making informative images for the ‘Research’-page <br/>
−
-Start with the design of hoodies <br/>
+
- Starting the design of hoodies <br/>
−
-Making funny images for our secret page </p>
+
- Photoshopping funny images for our secret page </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-We received lab material kindly given by KOLO Instruments - Paulussen Freddy <br/>
+
<p>- We received lab material kindly provided by KOLO Instruments - Paulussen Freddy <br/>
−
-Searching hotels in Bordeaux for the iGEM Meetup France 2015 <br/>
+
- Searching hotels in Bordeaux for the iGEM Meetup France 2015 <br/>
−
-Folders and brochures are ordered
+
- Ordering folders and brochures</p>
−
</p>
+
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Writing protocol for AHL detection <br/>
+
<p>- Writing protocols for AHL detection <br/>
−
-Writing abstract about literature on Wiki</p>
+
- Writing abstract about literature on Wiki</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Perform gel electrophoresis to confirm double knock-outs: 2 colonies of Δ<i>tar</i> Δ<i>cheZ</i> and 4 colonies of Δ<i>tar</i> Δ<i>tsr</i> still had Δ<i>tar</i>. This means we have our double mutants! We still need to check Δ<i>tar</i> Δ<i>cheZ</i> to be sure the rest of the operon is still intact. <br/>
+
<p>- Confirming the double knock-outs and checking the intactness <br/>
−
-We performed a motility test to verify the phenotypical change of knocking out <i>cheZ</i> <br/>
+
- Performing a motility test to verify the phenotypical change of knocking out <i>cheZ</i> <br/>
−
-Assembly of gBlocks by using the Gibson Assembly Method <br/>
+
- Assembling the gBlocks using the Gibson Assembly Method <br/>
−
-We decided to participate at the iGEM 2015 Measurement Interlab Study. Therefore we transformed <i>E. cloni</i> with the BioBricks J23101, I12504, J23106 and J23117.</p>
+
- Transforming <i>E. cloni</i> with the BioBricks J23101, I12504, J23106 and J23117 to participate in the iGEM 2015 Measurement Interlab Study.</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Write report about Simbiology <br/>
+
<p>- Writing a report about Simbiology <br/>
−
-Extending Hybrid Model to 2D </p>
+
- Extending the Hybrid Model to 2D </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Update Facebook & Twitter </p>
+
<p>- Updating Facebook & Twitter </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Contact potential keynote speakers for the symposium  </p>
+
<p>- Contacting potential keynote speakers for the symposium  </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-The History-page is online!</p>
+
<p>- Putting the History-page online</p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>-Making images for wiki-icons for subsections of the Newsfeed <br/>
+
<p>- Making images for wiki-icons for subsections of the Newsfeed <br/>
−
-Making tattoo-images to use as gadget </p>
+
- Making tattoo-images to use as gadgets </p>
 
     </div>
 
     </div>
 
   </div>
 
   </div>
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     </div>
 
     </div>
 
     <div class="partnews">
 
     <div class="partnews">
−
<p>- Making the protocol for plasmid assembly <br/>
+
<p>- Preparing the protocol for plasmid assembly <br/>
 
- Making the protocol for leucine detection <br/>
 
- Making the protocol for leucine detection <br/>
 
- Making protocol for AHL detection <br/>
 
- Making protocol for AHL detection <br/>

Revision as of 13:45, 17 September 2015

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Newsfeed

Week 11: the 7th-11th of September

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-On Monday, we had our ‘iGEM Symposium Day on Synthetic Biology, Cell Systems and Ethics in Biochemistry’.

team

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-We are constructing the BioBricks LuxI-His, RBS-LuxI-His, cheZ-GFP, RBS-cheZ-GFP, RBS-cheZ-GFP-RBS-PenI-Term-PpenI-RBS-RFP-Term by high fidelity tail-PCRn digestion and ligation in pSB1C3. We transformed these BioBricks in E. cloni and tested these colonies by PCR. The BioBrick RBS-cheZ-GFP-RBS-PenI-Term-PpenI-RBS-RFP-Term was confirmed by PCR and prepared for sequencing. The high fidelity PCR and cloning of other potential BioBricks was repeated.
-This week, we made our strains ΔtarΔtsr and ΔtarΔcheZ electrocompetent.
-Further, we would like to characterise a BioBrick. Therefore, we pasted RBS-cheZ-GFP after the promoter J23101. The idea is to test this BioBrick in a cheZ knock-out strain and to prove the presence of GFP.
-Our colonies containing the assembled gBlocks were checked by restriction mapping. The presence of the pUC vector in our samples made the restriction mapping complicated. This is why we chose to also investigate different samples who were treated with DpnI after the Gibson Assembly. The DpnI only cuts methylated DNA and thus only the original pUC. Unfortunately, the restriction mapping did not give the expected result.
-To have a backup plan, we also repeated the Gibson Assembly with pUC and the enzyme DpnI. After cloning and restriction mapping, we concluded that our sample contains only pUC.
-Our fourth gBlock is more or less 600 bp. To increase the amount and concentration of this gBlock, we performed a Phusion high fidelity PCR.

team

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-Hybrid model: implemented periodic boundary conditions
-Hybrid model: first draft of internal model incorporation into hybrid model
-Attended MATLAB webinar: Optimizing and Accelerating your MATLAB Code

team

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-Preparing games and presentations for school visits
-Visit the schools to teach children about synthetic biology
-Analyzing the results of our survey

team

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-Writing texts for on Wiki
-Our secret page is online!

team

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-Working on the design of the hoodies

Week 10: the 31thAugust-6th of September

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-We have new sponsors: Gips Mineral, Genzyme and VWR !
-We kindly received gadgets of our sponsors for use in our goody bag.
-Our bacteria-stickers are ordered

team

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-We obtained assembled gBlocks 1-2-3 and 5-6 in mini prepped form. On gel, we discovered that we have assembled gBlocks in pUC, but also a band at the height of pUC. We cut out the correct band, did a gel purification and transformed again.
-In parallel, we further tried to assemble gBlocks 1+2+3 with gBlock 4 by using two colonies who did not contain pUC. Later, we noticed by PCR that the assembly was not correct.
-We also tried to assemble gBlocks 1+2+3 with 5+6, this would give us the total plasmid for cell B. On gel, we saw that the restriction did not work. Probably, there was a problem with the restriction enzyme.

team

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-Collaboration with Toulouse: diffusion in Comsol
-Implementation of cells algorithm for nearest neighbor search
-Code optimization
-Meeting with Dirk Roose
-Examine effects of different contributions to cell movement in hybrid mode

team

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-Making presentation, scenario for our symposium.
-Practical arrangements for our symposium.
-Working on an educational game about synthetic biology

team

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-Adapting the team page: our mentors and advisors are online!
-Adapting our website for mobiles.
-Making a game for our secret pag.

team

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-Designing our iGEM banner
-Designing the print of our hoodies
-Adapting buttons of the wiki
-Designing the bacteria version of our promoter and supervisor
-Graphical design for the secret page

Week 8: the 17th-21th of August

team

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- We are in the track ‘New applications’
- Receiving offers for hoodies, stickers and tattoos
- Collaboration: Skype session with TU Delft
- Collaboration: Measuring pH of tap water and river water & sending samples to the iGEM team of York
- Collaboration: Interviewing people on the street for chewing-gum survey of the iGEM team of Aix-Marseille Université
- Translating our survey in French for further distribution in Wallonie.

team

hide

- Searching more information about lab safety
- Analyzing the results of the InterLab Measurement Study

team

hide

- Preparing electrocompetent cells
- Optimizing the tail PCR to make Biobricks
- Cloning the PCR products into pSB1C3, transforming the plasmids and screening of colonies to find the correct insert
- Transforming the devices for the Interlab Measurement Study
- Preparation of the standard curve based on fluorescein and measuring the fluorescence of the new devices
- Assembling the gBlocks using NEBuilder® HiFi DNA Assembly Master Mix and transforming the checked pcr products in electrocompetent E. cloni

team

hide

- Meeting with Tim Odenthal
- Optimization of hybrid model code
- Implementation of cell-cell interactions, including repulsion as well as attraction

team

hide

- Contacting keynote speakers for the Symposium
- Organizing symposium
- Mailing schools to give a playful course about synthetic biology
- Deciding on the rules for a nice card game about synthetic biology

team

hide

- Adapting pages
- Putting ‘Symposium’-page online

team

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- Making informative images for the Research-page
- Making buttons for the wiki
- Designing bacteria-stickers for use in schools and as gadgets
- Continuing with the design of our hoodies
- Designing funny images for our secret page on the wiki

Week 7: the 10th-14th of August

team

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- Our flyers and sponsor brochures arrived!
- Conducting a survey about synthetic biology interviewing people on the street
- Working on a team song

team

hide

- Making a protocol for leucine detection
- Researching lab safety

team

hide

- Switching from transformation of chemocompetent cells to electroporation due to low efficiency for the Interlab Measurement Study
- Checking the intactness of other genes in the tar-tap-cheRBYZ operon by PCR
- Ordering NEBuilder to repeat the failed Gibson Assembly
- Making three BioBricks starting from our gBlocks by tail PCR and restriction digestion
- Ordering materials for leucine and AHL detection

team

hide

- Finishing implementation of the hybrid model I in 2D, including ADI scheme for PDE part
- Extending hybrid model II to 2D
- Running hybrid model simulations
- Finishing report Simbiology
- Contacting Toulouse team for collaboration
- Contacting professors for possible collaboration

team

hide

- Brainstorming about educational games

team

hide

- Putting our ‘Newsfeed’ & ‘Research’-page online!
- Implementing an EasySwitch button and a Lightbox

team

hide

- Making informative images for the ‘Research’-page
- Starting the design of hoodies
- Photoshopping funny images for our secret page

Week 6: the 3th-7th of August

team

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- We received lab material kindly provided by KOLO Instruments - Paulussen Freddy
- Searching hotels in Bordeaux for the iGEM Meetup France 2015
- Ordering folders and brochures

team

hide

- Writing protocols for AHL detection
- Writing abstract about literature on Wiki

team

hide

- Confirming the double knock-outs and checking the intactness
- Performing a motility test to verify the phenotypical change of knocking out cheZ
- Assembling the gBlocks using the Gibson Assembly Method
- Transforming E. cloni with the BioBricks J23101, I12504, J23106 and J23117 to participate in the iGEM 2015 Measurement Interlab Study.

team

hide

- Writing a report about Simbiology
- Extending the Hybrid Model to 2D

team

hide

- Contacting potential keynote speakers for the symposium

team

hide

- Putting the History-page online

team

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- Making images for wiki-icons for subsections of the Newsfeed
- Making tattoo-images to use as gadgets

Week 5: the 27th-31th of July

team

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- Booking plane tickets to Boston
- Booking hotels Boston
- Two new companies are sponsoring: Eppendorf & LRD!

team

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- Preparing the protocol for plasmid assembly
- Making the protocol for leucine detection
- Making protocol for AHL detection
- Designing & ordering primers for the Gibson Assembly Method

team

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- Making double knock-out strains by P1 transduction
- Performing PCR and gel electrophoresis to confirm correct double knock-outs

team

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- Finishing the 2D models on a 100 by 100 grid

team

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- Contacting potential keynote speakers for the symposium
- Making a survey about public perception of synthetic biology
- Mailing the Bordeaux iGEM team for the France Meetup 2015

team

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- Putting the Modeling page online
- Adjusting the description of the project

team

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- Designing images for wiki team presentation
- Designing images for wiki icons for subsections of the Newsfeed
- Designing animations that represent our pattern forming bacteria

Week 4: the 20th-24th of July

team

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- Meeting the Toulouse iGEM team on 07/21/2015 at ESI (Expo Science International) in Brussels

team

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- Searching parameters necessary in mathematical model
- Designing and ordering the designed plasmid in gBlocks

team

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- Checking the removal of the kanamycin cassette in the Δtar strain and make a stock of our successful knockout
- Preparing phage P1 lysate to make the double knock-out strains

team

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- Simulating cell A and cell B in SymBiology
- Looking for usable constants
- Adapting the 2D continuous model

team

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- Inviting potential keynote speakers for the symposium

team

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- Adding the Team page

team

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- Making images for wiki team presentation
- Designing the flyer
- Making images of animals with new patterns for the wiki

Week 3: the 13th-17th of July

team

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- Constructing the plasmids
- Designing & ordering primers for controlling the knock-out proces
- Researching an alternative knock-out technique for double knockouts

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- Calculating the transformation efficiency of competent E. cloni cells
- Ordering 3 knock-out strains (Δtar, Δtsr and ΔcheZ) & preparing a stock
- Ordering Chromobacterium violaceum CV026 transposon mutant for usage in AHL detection & preparing a stock
- Removing the kanamycin resistance gene of the Δtar strain by transforming a plasmid with recombinase gene

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- Making and adapting the 1D hybrid and continuous model to the conditions of the wet lab
- Making a simple 2D continuous model
- Implementing biologically relevant parameters
- Making a 1D model with pdepe in Matlab
- Exploring symbiology
- Making a 2D model with the PDE toolbox in Matlab (not ready yet)

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- Preparing e-mail for symposium and contacting possible keynote speakers

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- Putting the description of our project online

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- Making images for the wiki

Week 2: the 6th-10th of July

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- Discussion with modeling team: which parameters do they need?
- Searching experiments for quantification of specific proteins, small molecules and amino acids
- Deciding on promoters of the plasmid
- Constructing the plasmids
- Making a working scheme (strategy)

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- Preparing LB agar medium
- Preparing competent cells (E. cloni) and testing their competency

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- Researching literature about hybrid models
- Further working on single cell agent-based model
- Implementing a simple one-dimensional hybrid model
- Exploring PDE Toolbox
- Working on an implicit continuous model
- Trying to simulate pattern formation of bacteria in COMSOL

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- First meeting about school projects
- Brainstorming about a symposium

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- Designing images and layout of wiki
- Designing images and brochure for sponsors

Week 1: the 1st-3th of July

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- Lab safety training
- Discussing tasks and practical arrangements (tickets Boston)
- Taking photos to use in the brochure, on the wiki and for social media
- Taking a tour through our high tech bio laboratory
- Meeting with potential sponsor

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- Searching for strains and BioBricks for our circuit

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- Setting up GitHub
- Constructing a simple single cell agent-based model
- Working on an explicit discretization of a continuous model

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- 'Coming soon' page is online

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- Designing images and layout of wiki
- Designing images and brochure for sponsors

History

Stroll down our memory lane !

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Go back to the Notebook page.
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History

Stroll down our memory lane.
Back

Go back to the Notebook page.

Contact

Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be