Difference between revisions of "Team:Valencia UPV/Results"

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<h2>Section 4<br />
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<h2>Results<br />
 
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<p>Hello world</p>
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<p>The concrete aim of AladDNA in this occasion is the production of one of the most needed drugs in the remote areas of the world. The production of our compounds was achive by transcient expression in Nicothiana Benthamiana leaves. </p>
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<p>Rotavirus Sip</p>
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<p>The sequence construction was kindly given to us by Juarez P. It was created from the variable region of an IgA created by them against rotavirus. However as the structure of small inmunoproteins contains some constant regions, two different constructions were given to us with different fragments of the constant region. The objective of this two structures is to achive which one is more affine to the capside protein of the virus as the affinity can vary as the conformational structure can change.</p>
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<p>Two leaves of Nicotiana were agroinfiltrated for each SIP and after three days samples were collected and a western blot inmuno assay was performed. Western membranes were probed with a labelled antibody against anti-rotavirus IgA.</p>
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<p>As it can be observed in the inmunodetection assay, only the SIP with the CH2 constant fragment was recognized by the anti-IgA against rotavirus. In the results there are two detection bands for the CH2 SIP the one with higher molecular weight corresponds to the complete protein produced by the plant. However there is a second band with lower molecular weight caused by the protein degradation that occurs also in the plant.
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Revision as of 14:32, 17 September 2015

Valencia UPV iGEM 2015

Section 1

Hello world


Sub section 1

Hello world

Section 2

Hello world

Section 3

Hello world

Results

The concrete aim of AladDNA in this occasion is the production of one of the most needed drugs in the remote areas of the world. The production of our compounds was achive by transcient expression in Nicothiana Benthamiana leaves.

Rotavirus Sip

The sequence construction was kindly given to us by Juarez P. It was created from the variable region of an IgA created by them against rotavirus. However as the structure of small inmunoproteins contains some constant regions, two different constructions were given to us with different fragments of the constant region. The objective of this two structures is to achive which one is more affine to the capside protein of the virus as the affinity can vary as the conformational structure can change.

Two leaves of Nicotiana were agroinfiltrated for each SIP and after three days samples were collected and a western blot inmuno assay was performed. Western membranes were probed with a labelled antibody against anti-rotavirus IgA.

As it can be observed in the inmunodetection assay, only the SIP with the CH2 constant fragment was recognized by the anti-IgA against rotavirus. In the results there are two detection bands for the CH2 SIP the one with higher molecular weight corresponds to the complete protein produced by the plant. However there is a second band with lower molecular weight caused by the protein degradation that occurs also in the plant.

Section 5

Hello world

Section 5

Hello world