Difference between revisions of "Team:HUST-China/Notebook"

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               font-size:16px;
 +
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Line 315: Line 316:
 
  </ul>
 
  </ul>
 
  </div>
 
  </div>
 +
 +
<!--收缩栏-->
 +
<div align="center" class="description"><a name="1"></a><br>
 +
    <div class="dongxi"></div>
 +
<div class="viewport container">
 +
<div class="main-content">
 +
<div class="bellows">
 +
<div class="bellows__item bellows--is-open">
 +
 +
<div class="bellows__header">
 +
<h2>Standalization</h2>
 +
</div>
 +
<div class="bellows__content">
 +
<!--  可以开始改了-->
 +
<h3>
 +
Week 1 6.29-7.5
 +
</h3>
 +
<h3>
 +
Week 2 7.6-7.12
 +
</h3>
 +
 +
<h3>
 +
Week 3 7.13-7.19
 +
</h3>
 +
 +
<h3>
 +
Week 4 7.20-7.26
 +
</h3>
 +
 +
<h3>
 +
Week 5 7.27-8.2
 +
</h3>
 +
 +
 +
 +
<h3>
 +
Week 6 8.3-8.9
 +
</h3>
 +
<p>
 +
PCR:
 +
<br>
 +
More details, protocol
 +
<br>
 +
WangJie Liu and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:350px;height:350px" src="https://static.igem.org/mediawiki/2015/0/09/Standar-1-form.png"  class="picture">
 +
<p>
 +
 +
</p>
 +
<img style="width:423px;height:200px" src="https://static.igem.org/mediawiki/2015/f/f6/Standar-1-form-1-pic.png"  class="picture">
 +
<br>
 +
 +
<img style="width:359px;height:347px" src="https://static.igem.org/mediawiki/2015/c/c7/Standar-1-form-2-pic.png"  class="picture">
 +
    <br>
 +
 +
<img style="width:600px;height:370px" src="https://static.igem.org/mediawiki/2015/8/8b/Standar-1-form-3-pic.png"  class="picture">
 +
<p>
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
WangJie Liu and JunJie Xu
 +
 +
</p>
 +
<img style="width:350px;height:350px" src="https://static.igem.org/mediawiki/2015/4/4c/Standar-2-form.png"  class="picture">
 +
<br>
 +
<img style="width:300px;height:300px" src="https://static.igem.org/mediawiki/2015/0/01/Standar-2-form-2-pic.png"  class="picture">
 +
<p>
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
WangJie Liu and JunJie Xu
 +
</p>
 +
 +
 +
<img style="width:400px;height:400px" src="https://static.igem.org/mediawiki/2015/4/4b/Standar-3-form.png"  class="picture">
 +
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
Colony PCR
 +
<br>
 +
WangJie Liu and JunJie Xu
 +
</p>
 +
 +
<p>
 +
 +
</p>
 +
<img style="width:400px;height:400px" src="https://static.igem.org/mediawiki/2015/f/fd/Standar-4-form.png"  class="picture"><br>
 +
<img style="width:400px;height:400px" src="https://static.igem.org/mediawiki/2015/c/c5/Standar-4-form-1-pic.png"  class="picture">
 +
<br>
 +
<img style="width:340px;height:240px" src="https://static.igem.org/mediawiki/2015/1/1f/Standar-4-form-2-pic.png"  class="picture">
 +
<br>
 +
<img style="width:730px;height:330px" src="https://static.igem.org/mediawiki/2015/9/90/Standar-4-form-3-pic.png"  class="picture">
 +
<br>
 +
<img style="width:420px;height:400px" src="https://static.igem.org/mediawiki/2015/5/56/Standar-4-form-4-pic.png"  class="picture">
 +
<br>
 +
<img style="width:350px;height:270px" src="https://static.igem.org/mediawiki/2015/6/66/Standar-4-form-5-pic.png"  class="picture">
 +
<br>
 +
 +
<p>
 +
Extract the plasmid,Conserving.
 +
</p>
 +
 +
<h3>
 +
Week 7 8.10-8.16
 +
</h3>
 +
<p>
 +
PCR:
 +
<br>
 +
More details, protocol
 +
<br>
 +
WangJie Liu
 +
<br>
 +
</p>
 +
<img style="width:450px;height:350px" src="https://static.igem.org/mediawiki/2015/3/34/Standar-5-form.png"  class="picture">
 +
<p>
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
WangJie Liu
 +
<br>
 +
</p>
 +
<img style="width:450px;height:350px" src="https://static.igem.org/mediawiki/2015/f/f9/Standar-6-form.png"  class="picture">
 +
<br>
 +
<img style="width:450px;height:350px" src="https://static.igem.org/mediawiki/2015/5/5b/Standar-5-form-1-pic.png"  class="picture">
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
WangJie Liu
 +
<br>
 +
</p>
 +
 +
 +
<img style="width:550px;height:300px" src="https://static.igem.org/mediawiki/2015/9/98/Standar-7-form.png"  class="picture">
 +
<p>
 +
Colony PCR
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
WangJie Liu
 +
<br>
 +
 +
</p>
 +
<img style="width:550px;height:350px" src="https://static.igem.org/mediawiki/2015/2/2d/Standar-8-form.png"  class="picture">
 +
<p>
 +
<p>
 +
Extract the plasmid,Conserving.
 +
<br>
 +
</p>
 +
<h3>
 +
Week 8 8.17-8.23
 +
</h3>
 +
<img style="width:350px;height:450px" src="https://static.igem.org/mediawiki/2015/a/a7/S-l-W8P1.png"  class="picture">
 +
<br>
 +
<img style="width:350px;height:350px" src="https://static.igem.org/mediawiki/2015/1/1d/S-l-W8P2.png"  class="picture">
 +
<br>
 +
<img style="width:500px;height:350px" src="https://static.igem.org/mediawiki/2015/f/fc/S-l-W8P3.png"  class="picture">
 +
<br>
 +
<img style="width:450px;height:250px" src="https://static.igem.org/mediawiki/2015/a/a1/S-l-W8P4.png"  class="picture">
 +
 +
<h3>
 +
Week 9 8.24-8.30
 +
</h3>
 +
<img style="width:500px;height:390px" src="https://static.igem.org/mediawiki/2015/2/27/S-l-W9P1.png"  class="picture">
 +
<h3>
 +
Week 10 8.31-9.6
 +
</h3>
 +
<p>
 +
Successfully align the sequencing results with the objective sequences
 +
<br>
 +
Standalization of BD-CRY2 and AD-CIB1 finished.
 +
<br>
 +
 +
</p>
 +
<img style="width:350px;height:250px" src="https://static.igem.org/mediawiki/2015/d/da/S-l-W10P1.png"  class="picture">
 +
<br>
 +
<img style="width:450px;height:260px"src="https://static.igem.org/mediawiki/2015/8/89/S-l-W10P2.png"  class="picture">
 +
<br>
 +
<img style="width:450px;height:260px" src="https://static.igem.org/mediawiki/2015/1/1b/S-l-W10P3.png"  class="picture">
 +
<br>
 +
<img style="width:510px;height:450px" src="https://static.igem.org/mediawiki/2015/4/4a/S-l-W10P4.png"  class="picture">
 +
<p>
 +
Successfully align the sequencing results with the objective sequences
 +
<br>
 +
Standalization of CRY2 and CIB1 finished.
 +
<br>
 +
</p>
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
<div class="bellows__item">
 +
<div class="bellows__header">
 +
<h2>Light Control</h2>
 +
</div>
 +
<div class="bellows__content">
 +
<h3>
 +
Week 1 6.29-7.5
 +
</h3>
 +
 +
<h3>
 +
Week 2 7.6-7.12
 +
</h3>
 +
<img style="width:450px;height:300px" src="https://static.igem.org/mediawiki/2015/1/15/L-prt-W2P1.png"  class="picture">
 +
<br>
 +
<img style="width:450px;height:300px" src="https://static.igem.org/mediawiki/2015/1/19/L-prt-W2P2.png"  class="picture">
 +
<br>
 +
<img style="width:500px;height:250px" src="https://static.igem.org/mediawiki/2015/6/64/L-prt-W2P3.png"  class="picture">
 +
<br>
 +
<img style="width:570px;height:420px" src="https://static.igem.org/mediawiki/2015/4/41/L-prt-W2P4.png"  class="picture">
 +
<img style="width:630px;height:720px" src="https://static.igem.org/mediawiki/2015/4/4f/L-prt-W2P5.png"  class="picture">
 +
<p>
 +
Successfully align the sequencing results with the objective sequences of Pgal1 and CYC_TT.
 +
<br>
 +
Extract the plasmid.
 +
<br>
 +
Conserving.
 +
 +
<br>
 +
</p>
 +
 +
 +
<h3>
 +
Week 3 7.13-7.19
 +
</h3>
 +
 +
<h3>
 +
Week 4 7.20-7.26
 +
</h3>
 +
<br> <img style="width:450px;height:250px" src="https://static.igem.org/mediawiki/2015/9/9b/L-prt-W4P1.png"  class="picture">
 +
<br> <img style="width:450px;height:250px" src="https://static.igem.org/mediawiki/2015/8/8d/L-prt-W4P2.png"  class="picture">
 +
<br>
 +
<img style="width:570px;height:300px" src="https://static.igem.org/mediawiki/2015/d/dd/L-prt-W4P3.png"  class="picture">
 +
 +
 +
<h3>
 +
Week 5 7.27-8.2
 +
</h3>
 +
<p>
 +
Successfully align the sequencing results with the objective sequences <br>
 +
</p>
 +
<img style="width:420px;height:270px" src="https://static.igem.org/mediawiki/2015/9/94/L-prt-W5P1.png"  class="picture">
 +
<br>
 +
<img style="width:450px;height:265px" src="https://static.igem.org/mediawiki/2015/3/3e/L-prt-W5P2.png"  class="picture">
 +
<br>
 +
<img style="width:430px;height:350px" src="https://static.igem.org/mediawiki/2015/7/71/L-prt-W5P3.png"  class="picture">
 +
<br>
 +
<img style="width:560px;height:270px" src="https://static.igem.org/mediawiki/2015/1/1d/L-prt-W5P4.png"  class="picture">
 +
<br>
 +
<img style="width:520px;height:230px" src="https://static.igem.org/mediawiki/2015/5/5b/L-prt-W5P5.png"  class="picture">
 +
<p>
 +
Digestion Test
 +
<br>
 +
Guozhao Wu
 +
<br>
 +
</p>
 +
 +
<h3>
 +
Week 6 8.3-8.9
 +
</h3>
 +
<img style="width:400px;height:200px" src="https://static.igem.org/mediawiki/2015/8/8a/L-prt-W6P1.png"  class="picture">
 +
<br>
 +
<img style="width:460px;height:250px" src="https://static.igem.org/mediawiki/2015/f/fb/L-prt-W6P2.png"  class="picture">
 +
<br>
 +
<img style="width:490px;height:250px" src="https://static.igem.org/mediawiki/2015/8/8a/L-prt-W6P3.png"  class="picture">
 +
<p>
 +
Successfully align the sequencing results with the objective sequences
 +
<br>
 +
Pgal-ROX1-CYC_TT circuit was constructed.
 +
<br>
 +
</p>
 +
<h3>
 +
Week 7 8.10-8.16
 +
</h3>
 +
 +
<h3>
 +
Week 8 8.17-8.23
 +
</h3>
 +
 +
<h3>
 +
Week 9 8.24-8.30
 +
</h3>
 +
 +
<h3>
 +
Week 10 8.31-9.6
 +
</h3>
 +
 +
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
</div>
 +
<div class="bellows__item">
 +
<div class="bellows__header">
 +
<h2>Viscous Parts</h2>
 +
</div>
 +
<div class="bellows__content">
 +
<h3>
 +
Week 1 6.29-7.5
 +
</h3>
 +
 +
<h3>
 +
Week 2 7.6-7.12
 +
</h3>
 +
<p>
 +
PCR:
 +
<br>
 +
More details, protocol
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:400px;height:400px" src="https://static.igem.org/mediawiki/2015/0/0e/STMcfp-1-form.png"  class="picture">
 +
<br>
 +
<img style="width:320px;height:250px" src="https://static.igem.org/mediawiki/2015/c/c0/STMcfp-1-form-1-pic.png"  class="picture">
 +
<br>
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:400px;height:400px" src="https://static.igem.org/mediawiki/2015/6/67/STMcfp-2-form.png"  class="picture">
 +
<br>
 +
<img style="width:400px;height:300px" src="https://static.igem.org/mediawiki/2015/9/95/STMcfp-2-form-1-pic.png"  class="picture">
 +
<br>
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:500px;height:400px" src="https://static.igem.org/mediawiki/2015/f/f9/STMcfp-3-form.png"  class="picture">
 +
<p>
 +
Colony PCR
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:260px;height:423px" src="https://static.igem.org/mediawiki/2015/b/b7/STMcfp-4-form.png"  class="picture">
 +
<br>
 +
<img style="width:500px;height:290px" src="https://static.igem.org/mediawiki/2015/7/71/STMcfp-4-form-1-pic.png"  class="picture">
 +
<br>
 +
<img style="width:440px;height:300px" src="https://static.igem.org/mediawiki/2015/b/bf/STMcfp-4-form-2-pic.png"  class="picture">
 +
<br>
 +
<p>
 +
Extract the plasmid,Conserving<br>
 +
</p>
 +
 +
<h3>
 +
Week 3 7.13-7.19
 +
</h3>
 +
<p>
 +
PCR:
 +
 +
<br>
 +
More details, protocol
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:420px;height:340px" src="https://static.igem.org/mediawiki/2015/b/be/STMcfp-5-form.png"  class="picture">
 +
<br>
 +
<img style="width:340px;height:200px" src="https://static.igem.org/mediawiki/2015/a/a0/STMcfp-5-form-1-pic.png"  class="picture">
 +
<br>
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:420px;height:400px" src="https://static.igem.org/mediawiki/2015/6/64/STMcfp-6-form.png"  class="picture">
 +
<br>
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:800px;height:200px" src="https://static.igem.org/mediawiki/2015/0/07/STMcfp-7-form.png"  class="picture">
 +
 +
<p>
 +
Colony PCR
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
</p>
 +
<img style="width:480px;height:320px" src="https://static.igem.org/mediawiki/2015/c/c3/STMcfp-8-form.png"  class="picture">
 +
<br>
 +
<img style="width:250px;height:300px" src="https://static.igem.org/mediawiki/2015/9/93/STMcfp-8-form-1-pic.png"  class="picture">
 +
<p>
 +
Extract the plasmid,Conserving.<br>
 +
</p>
 +
<h3>
 +
Week 4 7.20-7.26
 +
</h3>
 +
<p>
 +
PCR:
 +
<br>
 +
More details, protocol
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:420px;height:200px" src="https://static.igem.org/mediawiki/2015/c/ce/STMcfp-9-form.png"  class="picture">
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
</p>
 +
<img style="width:420px;height:300px" src="https://static.igem.org/mediawiki/2015/c/cc/STMcfp-10-form.png"  class="picture">
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
Wang Xi and ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:620px;height:430px" src="https://static.igem.org/mediawiki/2015/e/ec/STMcfp-11-form.png"  class="picture">
 +
<p>
 +
Colony PCR
 +
<br>
 +
Wang Xi and JunJie Xu
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
</p>
 +
<img style="width:470px;height:250px" src="https://static.igem.org/mediawiki/2015/8/83/STMcfp-12-form.png"  class="picture">
 +
<br>
 +
<img style="width:300px;height:300px" src="https://static.igem.org/mediawiki/2015/f/f2/STMcfp-12-form-1-pic.png"  class="picture">
 +
<p>
 +
Extract the plasmid,Conserving.
 +
</p>
 +
<p>
 +
Tramsformation to yeast
 +
<br>
 +
Lei Yin
 +
<br>
 +
</p>
 +
<img style="width:440px;height:480px" src="https://static.igem.org/mediawiki/2015/0/0d/STMcfp-13-form.png"  class="picture">
 +
<p>
 +
Genome extract and PCR test
 +
<br>
 +
Lei Yin
 +
<br>
 +
</p>
 +
<img style="width:390px;height:480px" src="https://static.igem.org/mediawiki/2015/c/c8/STMcfp-14-form.png"  class="picture">
 +
<p>
 +
Positive strain conservation
 +
<br>
 +
</p>
 +
<h3>
 +
Week 5 7.27-8.2
 +
</h3>
 +
<p>
 +
PCR:
 +
<br>
 +
More details, protocol
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:420px;height:340px" src="https://static.igem.org/mediawiki/2015/4/46/STMcfp-15-form.png"  class="picture">
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:420px;height:380px" src="https://static.igem.org/mediawiki/2015/d/d1/STMcfp-16-form.png"  class="picture">
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:620px;height:280px" src="https://static.igem.org/mediawiki/2015/2/20/STMcfp-17-form.png"  class="picture">
 +
<p>
 +
Colony PCR
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
</p>
 +
<img style="width:470px;height:340px" src="https://static.igem.org/mediawiki/2015/6/63/STMcfp-18-form.png"  class="picture">
 +
<br>
 +
<img style="width:420px;height:370px" src="https://static.igem.org/mediawiki/2015/1/17/STMcfp-18-form-1-pic.png"  class="picture">
 +
<br>
 +
<img style="width:300px;height:370px" src="https://static.igem.org/mediawiki/2015/0/0a/STMcfp-18-form-2-pic.png"  class="picture">
 +
<br>
 +
<img style="width:510px;height:380px" src="https://static.igem.org/mediawiki/2015/0/0d/STMcfp-18-form-3-pic.png"  class="picture">
 +
<br>
 +
<img style="width:400px;height:400px" src="https://static.igem.org/mediawiki/2015/a/a0/STMcfp-18-form-4-pic.png"  class="picture">
 +
<p>
 +
Extract the plasmid,Conserving.
 +
<br>
 +
</p>
 +
<p>
 +
Trasnsform to yeast
 +
<br>
 +
</p>
 +
<img style="width:500px;height:340px" src="https://static.igem.org/mediawiki/2015/a/a4/STMcfp-19-form.png"  class="picture">
 +
<p>
 +
Genome extract and PCR test
 +
<br>
 +
Lei Yin
 +
<br>
 +
</p>
 +
<img style="width:390px;height:340px" src="https://static.igem.org/mediawiki/2015/c/c2/STMcfp-20-form.png"  class="picture">
 +
<p>
 +
Positive strain conservation
 +
<br>
 +
</p>
 +
<h3>
 +
Week 6 8.3-8.9
 +
</h3>
 +
<p>
 +
PCR:
 +
<br>
 +
More details, protocol
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:420px;height:240px" src="https://static.igem.org/mediawiki/2015/9/99/STMcfp-21-form.png"  class="picture">
 +
<p>
 +
Restriction Enzyme Digestion
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:420px;height:250px" src="https://static.igem.org/mediawiki/2015/5/5d/STMcfp-22-form.png"  class="picture">
 +
<p>
 +
DNA ligation&Transformation
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:660px;height:250px" src="https://static.igem.org/mediawiki/2015/a/a9/STMcfp-23-form.png"  class="picture">
 +
<p>
 +
Colony PCR
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
Successfully align the sequencing results with the objective sequences:
 +
<br>
 +
</p>
 +
<img style="width:470px;height:240px" src="https://static.igem.org/mediawiki/2015/e/e9/STMcfp-24-form.png"  class="picture">
 +
<br>
 +
<img style="width:380px;height:250px" src="https://static.igem.org/mediawiki/2015/a/ad/STMcfp-24-form-1-pic.png"  class="picture">
 +
<br>
 +
<img style="width:250px;height:220px" src="https://static.igem.org/mediawiki/2015/a/a8/STMcfp-24-form-2-pic.png"  class="picture">
 +
<p>
 +
Extract the plasmid,Conserving.
 +
<br>
 +
</p>
 +
<p>
 +
Trasnsform to yeast
 +
<br>
 +
Lei Yin
 +
<br>
 +
</p>
 +
<img style="width:440px;height:260px" src="https://static.igem.org/mediawiki/2015/d/d4/STMcfp-25-form.png"  class="picture">
 +
<p>
 +
Genome extract and PCR test
 +
<br>
 +
Lei Yin
 +
<br>
 +
</p>
 +
<img style="width:400px;height:240px" src="https://static.igem.org/mediawiki/2015/2/21/STMcfp-26-form.png"  class="picture">
 +
<p>
 +
Positive strain conservation<br>
 +
</p>
 +
<h3>
 +
Week 7 8.10-8.16
 +
</h3>
 +
<p>
 +
SDS-PAGE
 +
<br>
 +
Lei Yin
 +
<br>
 +
</p>
 +
<img style="width:300px;height:150px" src="https://static.igem.org/mediawiki/2015/6/65/STMcfp-27-form.png"  class="picture">
 +
<p>
 +
Immunoflourecense
 +
<br>
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<img style="width:400px;height:320px" src="https://static.igem.org/mediawiki/2015/a/ac/STMcfp-28-form.png"  class="picture">
 +
<p>
 +
Verification of the secreting Mcfp-3 (Coomassie blue staining)  <br>
 +
 +
ZhangYu Cheng
 +
<br>
 +
</p>
 +
<h3>
 +
Week 8 8.17-8.23
 +
</h3>
 +
 +
<h3>
 +
Week 9 8.24-8.30
 +
</h3>
 +
 +
<h3>
 +
Week 10 8.31-9.6
 +
</h3>
 +
</div>
 +
</div>
 +
<div class="bellows__item">
 +
<div class="bellows__header">
 +
<h2>Improvement</h2>
 +
</div>
 +
<div class="bellows__content">
 +
<h3>
 +
Week 1 6.29-7.5
 +
</h3>
 +
 +
<h3>
 +
Week 2 7.6-7.12
 +
</h3>
 +
 +
<h3>
 +
Week 3 7.13-7.19
 +
</h3>
 +
<img style="width:650px;height:300px" src="https://static.igem.org/mediawiki/2015/3/3f/I-W3P1.png"  class="picture">
 +
<br>
 +
<img style="width:520px;height:500px" src="https://static.igem.org/mediawiki/2015/3/39/I-W3P2.png"  class="picture">
 +
<h3>
 +
Week 4 7.20-7.26
 +
</h3>
 +
 +
<h3>
 +
Week 5 7.27-8.2
 +
</h3>
 +
<img style="width:620px;height:300px" src="https://static.igem.org/mediawiki/2015/4/49/I-W5P1.png"  class="picture">
 +
<br>
 +
<img style="width:470px;height:300px" src="https://static.igem.org/mediawiki/2015/d/dd/I-W5P2.png"  class="picture">
 +
<br>
 +
<img style="width:460px;height:300px" src="https://static.igem.org/mediawiki/2015/e/e3/I-W5P3.png"  class="picture">
 +
<br>
 +
<img style="width:530px;height:490px" src="https://static.igem.org/mediawiki/2015/3/33/I-W5P4.png"  class="picture">
 +
<br>
 +
<img style="width:280px;height:400px" src="https://static.igem.org/mediawiki/2015/6/6d/I-W5P5.png"  class="picture">
 +
<h3>
 +
Week 6 8.3-8.9
 +
</h3>
 +
<img style="width:500px;height:340px" src="https://static.igem.org/mediawiki/2015/e/ee/I-W6P1.png"  class="picture">
 +
<br>
 +
<img style="width:340px;height:330px" src="https://static.igem.org/mediawiki/2015/0/02/I-W6P2.png"  class="picture">
 +
<br>
 +
<img style="width:300px;height:270px" src="https://static.igem.org/mediawiki/2015/1/17/I-W6P3.png"  class="picture">
 +
<br>
 +
<img style="width:500px;height:500px" src="https://static.igem.org/mediawiki/2015/d/df/I-W6P4.png"  class="picture">
 +
<h3>
 +
Week 7 8.10-8.16
 +
</h3>
 +
 +
<h3>
 +
Week 8 8.17-8.23
 +
</h3>
 +
 +
<h3>
 +
Week 9 8.24-8.30
 +
</h3>
 +
 +
<h3>
 +
Week 10 8.31-9.6
 +
</h3>
 +
</div>
 +
 +
<div class="bellows__item">
 +
<div class="bellows__header">
 +
<h2>Consolidation</h2>
 +
</div>
 +
<div class="bellows__content">
 +
<h3>
 +
 +
Week 1 6.29-7.5
 +
</h3>
 +
 +
<h3>
 +
Week 2 7.6-7.12
 +
                                </h3>
 +
 +
                                <h3>
 +
Week 3 7.13-7.19
 +
</h3>
 +
 +
<h3>
 +
Week 4 7.20-7.26
 +
</h3>
 +
                                <h3>
 +
Week 5 7.27-8.2
 +
</h3>
 +
 +
<h3>
 +
Week 6 8.3-8.9
 +
</h3>
 +
 +
<h3>
 +
Week 7 8.10-8.16
 +
</h3>
 +
 +
<h3>
 +
Week 8 8.17-8.23
 +
</h3>
 +
 +
<h3>
 +
Week 9 8.24-8.30
 +
</h3>
 +
 +
<h3>
 +
Week 10 8.31-9.6
 +
</h3>
 +
                                <img style="width:600px;height:400px" src="https://static.igem.org/mediawiki/2015/6/67/STYZ1.png"  class="picture">
 +
<br>
 +
                                <img style="width:650px;height:400px" src="https://static.igem.org/mediawiki/2015/d/d4/STYZ2.png"  class="picture">
 +
<br>
 +
                                <img style="width:650px;height:400px" src="https://static.igem.org/mediawiki/2015/8/82/STYZ3.png"  class="picture">
 +
<br>
 +
                                <img style="width:650px;height:400px" src="https://static.igem.org/mediawiki/2015/8/88/STYZ4.png"  class="picture">
 +
 +
</div>
 +
</div>
 +
</div>
 +
 +
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Revision as of 14:50, 17 September 2015

Team:HUST-China:Description


Scroll down to read more


Standalization

Week 1 6.29-7.5

Week 2 7.6-7.12

Week 3 7.13-7.19

Week 4 7.20-7.26

Week 5 7.27-8.2

Week 6 8.3-8.9

PCR:
More details, protocol
WangJie Liu and JunJie Xu



Restriction Enzyme Digestion
WangJie Liu and JunJie Xu


DNA ligation&Transformation
WangJie Liu and JunJie Xu


Successfully align the sequencing results with the objective sequences:
Colony PCR
WangJie Liu and JunJie Xu







Extract the plasmid,Conserving.

Week 7 8.10-8.16

PCR:
More details, protocol
WangJie Liu

Restriction Enzyme Digestion
WangJie Liu


DNA ligation&Transformation
WangJie Liu

Colony PCR
Successfully align the sequencing results with the objective sequences:
WangJie Liu

Extract the plasmid,Conserving.

Week 8 8.17-8.23




Week 9 8.24-8.30

Week 10 8.31-9.6

Successfully align the sequencing results with the objective sequences
Standalization of BD-CRY2 and AD-CIB1 finished.




Successfully align the sequencing results with the objective sequences
Standalization of CRY2 and CIB1 finished.

Light Control

Week 1 6.29-7.5

Week 2 7.6-7.12




Successfully align the sequencing results with the objective sequences of Pgal1 and CYC_TT.
Extract the plasmid.
Conserving.

Week 3 7.13-7.19

Week 4 7.20-7.26




Week 5 7.27-8.2

Successfully align the sequencing results with the objective sequences





Digestion Test
Guozhao Wu

Week 6 8.3-8.9



Successfully align the sequencing results with the objective sequences
Pgal-ROX1-CYC_TT circuit was constructed.

Week 7 8.10-8.16

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6

Viscous Parts

Week 1 6.29-7.5

Week 2 7.6-7.12

PCR:
More details, protocol
Wang Xi and JunJie Xu



Restriction Enzyme Digestion
Wang Xi and JunJie Xu



DNA ligation&Transformation
Wang Xi and JunJie Xu

Colony PCR
Successfully align the sequencing results with the objective sequences:
Wang Xi and JunJie Xu




Extract the plasmid,Conserving

Week 3 7.13-7.19

PCR:
More details, protocol
ZhangYu Cheng



Restriction Enzyme Digestion
ZhangYu Cheng


DNA ligation&Transformation
ZhangYu Cheng

Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:


Extract the plasmid,Conserving.

Week 4 7.20-7.26

PCR:
More details, protocol
Wang Xi and JunJie Xu

Restriction Enzyme Digestion
Wang Xi and JunJie Xu

DNA ligation&Transformation
Wang Xi and ZhangYu Cheng

Colony PCR
Wang Xi and JunJie Xu
Successfully align the sequencing results with the objective sequences:


Extract the plasmid,Conserving.

Tramsformation to yeast
Lei Yin

Genome extract and PCR test
Lei Yin

Positive strain conservation

Week 5 7.27-8.2

PCR:
More details, protocol
ZhangYu Cheng

Restriction Enzyme Digestion
ZhangYu Cheng

DNA ligation&Transformation
ZhangYu Cheng

Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:





Extract the plasmid,Conserving.

Trasnsform to yeast

Genome extract and PCR test
Lei Yin

Positive strain conservation

Week 6 8.3-8.9

PCR:
More details, protocol
ZhangYu Cheng

Restriction Enzyme Digestion
ZhangYu Cheng

DNA ligation&Transformation
ZhangYu Cheng

Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:



Extract the plasmid,Conserving.

Trasnsform to yeast
Lei Yin

Genome extract and PCR test
Lei Yin

Positive strain conservation

Week 7 8.10-8.16

SDS-PAGE
Lei Yin

Immunoflourecense
ZhangYu Cheng

Verification of the secreting Mcfp-3 (Coomassie blue staining)
ZhangYu Cheng

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6

Improvement

Week 1 6.29-7.5

Week 2 7.6-7.12

Week 3 7.13-7.19


Week 4 7.20-7.26

Week 5 7.27-8.2





Week 6 8.3-8.9




Week 7 8.10-8.16

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6

Consolidation

Week 1 6.29-7.5

Week 2 7.6-7.12

Week 3 7.13-7.19

Week 4 7.20-7.26

Week 5 7.27-8.2

Week 6 8.3-8.9

Week 7 8.10-8.16

Week 8 8.17-8.23

Week 9 8.24-8.30

Week 10 8.31-9.6