Difference between revisions of "Team:Warwick/Modelling2"
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<p style="float: right;"><img src="https://static.igem.org/mediawiki/2015/4/4e/Warwickh.png" align="right" height="150px" width="150px" border="1px"></p> | <p style="float: right;"><img src="https://static.igem.org/mediawiki/2015/4/4e/Warwickh.png" align="right" height="150px" width="150px" border="1px"></p> | ||
<p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/7/74/WarwickE.coli_Bonded_to_Origami.png" height="300px" width="300px" border="1px"></p> | <p style="float: left;"><img src="https://static.igem.org/mediawiki/2015/7/74/WarwickE.coli_Bonded_to_Origami.png" height="300px" width="300px" border="1px"></p> | ||
| − | The image on the left shows how the E.coli will bond to the DNA Origami structures. We can choose what zinc fingers go on what end of the structures so we could have a pattern in the origami structure. This is useful for analysing microbial communities as it allows different cell types to be brought together. | + | The image on the left shows how the <i>E.coli</i> will bond to the DNA Origami structures. We can choose what zinc fingers go on what end of the structures so we could have a pattern in the origami structure. This is useful for analysing microbial communities as it allows different cell types to be brought together. |
<br>It would be possible to create 2D and 3D structures using these Origami structures as a glue to hold the cells together but would require hundreds of different zinc fingers to prevent the wrong parts being bonded to one another. | <br>It would be possible to create 2D and 3D structures using these Origami structures as a glue to hold the cells together but would require hundreds of different zinc fingers to prevent the wrong parts being bonded to one another. | ||
| − | <br>This shows how a simple shape could be made by using E.coli (black squares) by connecting them with DNA origami (red crosses). In order for a shape to be made each piece of E.coli needs to express a different zinc finger so that it can only be bonded to a specific piece of origami (no non-specific bonding). | + | <br>This shows how a simple shape could be made by using <i>E.coli</i> (black squares) by connecting them with DNA origami (red crosses). In order for a shape to be made each piece of <i>E.coli</i> needs to express a different zinc finger so that it can only be bonded to a specific piece of origami (no non-specific bonding). |
<br>We only have four zinc fingers which means that we don’t have many options for patterns we could make, but given enough time and resources we could easily optimise more zinc fingers so more complex shapes could be made. | <br>We only have four zinc fingers which means that we don’t have many options for patterns we could make, but given enough time and resources we could easily optimise more zinc fingers so more complex shapes could be made. | ||
<br>Future iGEM teams could create more zinc fingers which could be combined with our structures so that as time progresses a database of different shaped and sized oligonucleotide adhesives can be made. Our project could then be used as a stepping stone to create complex 2D and eventually 3D shapes and structures. | <br>Future iGEM teams could create more zinc fingers which could be combined with our structures so that as time progresses a database of different shaped and sized oligonucleotide adhesives can be made. Our project could then be used as a stepping stone to create complex 2D and eventually 3D shapes and structures. | ||
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<h5 class="sidebartitle">Minimum Size of Plasmids</h5> | <h5 class="sidebartitle">Minimum Size of Plasmids</h5> | ||
<p> | <p> | ||
| − | It is paramount that the length of the plasmid arms are kept to a minimum length as the longer the arms the more unstable the resulting structure will be. It would also take a longer time to form and would have a lower probability of formation. However if the plasmid arms are kept to the smallest possible size it decreases the likelihood of the correct number of E.coli cells bonding to the ends (we have assumed that the ends of the E.coli are perfect spheres and will bond in the centre- if this is not the case the you will need an extra length to accommodate. We calculated 30% would be the optimum error margin to add). <br> Obviously calculating the plasmid sizes is very important then as it dictates cost and efficiency. The cube construction page explains how this was done. | + | It is paramount that the length of the plasmid arms are kept to a minimum length as the longer the arms the more unstable the resulting structure will be. It would also take a longer time to form and would have a lower probability of formation. However if the plasmid arms are kept to the smallest possible size it decreases the likelihood of the correct number of <i>E.coli</i> cells bonding to the ends (we have assumed that the ends of the <i>E.coli</i> are perfect spheres and will bond in the centre- if this is not the case the you will need an extra length to accommodate. We calculated 30% would be the optimum error margin to add). <br> Obviously calculating the plasmid sizes is very important then as it dictates cost and efficiency. The cube construction page explains how this was done. |
</p> | </p> | ||
Revision as of 14:56, 17 September 2015
This shows how the DNA strands come together. Three double stranded strings of DNA are denatured and then when slowly cooled will come together to form the Y shape. However after the denaturing each strand of DNA has an equal chance of bonding to the original piece of DNA as it does to the correct origami side. Therefore the more complex the structure the less likely it is that that structure will fully form.
