Difference between revisions of "Team:Bordeaux/Yeast results"
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<h5 align="center"> <b><i>Saccharomyces cerevisiae</i> RESULTS </b></h5> | <h5 align="center"> <b><i>Saccharomyces cerevisiae</i> RESULTS </b></h5> | ||
− | <br> | + | <p align="justify"> <br> The strategy of overexpressing FKS1 was a failure. Our production results show a dramatical decrease of the Curdlan. It can mean we « broke » the gene during the experiment. Despite these bad results we managed to produce a Biobrick that can be use for every expression in <I>Saccharomyces Cerevisae</I>. |
− | + | <br> <b> Successful results: </b> | |
+ | <br> - Cloning in pSB1C3 | ||
+ | <br> - Transformation of IVSc1 strain | ||
+ | <br> - Curdlan production in wild type | ||
+ | <br> <br> | ||
+ | <b> Unsuccessful results: </b> | ||
+ | <br> - No PCR amplification of FKS1 | ||
+ | <br> - No increase of production | ||
+ | <br> <br> | ||
+ | <b> Perspectives: </b> | ||
+ | <br> - Sulfation of our produced Curdlan | ||
+ | <br> - Seperation of beta1,3 and beta 1,6 glucans | ||
+ | <br> - Optimization of curdlan purification steps | ||
+ | <br> - Tests on plants | ||
+ | </p> | ||
Revision as of 16:27, 17 September 2015