Difference between revisions of "Template:Team:Groningen/CONTENT/LOGBOOK/Restriction th3"
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Revision as of 16:42, 17 September 2015
Restriction (th3)
Digestion of pdg1664
extra integration of DNA in thrC locus
Plasmid was digest and purified.
<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/restriction">Restriction</a>
00:00, 26 Augustus 2015 - 00:00, 26 Augustus 2015
pdg1664 plasmid DNA was digested with EcoRI and AatII for 2 hours at 37 °C. The restriction samples were run on a 1% agarose gel and correct bands were cut out of the gel and purified with the PCR purification kit from Thermo Scientific.