Collaborations

Collaboration is an important part of the iGEM competition. We worked with three different 2015 iGEM teams to achieve different goals.

Valencia (UPV) team

The Valencia (UPV) team are also working on plants (along with the Cambridge team we are participating in the Plant Workshop at the Giant Jamboree).

We also received plant parts from Valencia-UPV, which we tested in the plant chassis, Nicotiana tabacum, in a transient assay. We recorded the result using confocal microscopy. We also sent them the ‘MoClo Flipper Constructs’ made by the 2014 NRP-UEA allowing them to easily convert their GoldenGate parts to standardised BioBricks. The Valencia-UPV team helped us by testing our plant-expression constructs in their laboratory.

You can learn more by clicking on the image on the right.

TU_Eindhoven team

The The Tu-Eindhoven team are compiling a ‘Cloning Guide’ for future iGEM teams. We have contributed a chapter on Golden Gate Cloning, which we used for the assembly of parts into plant expression vectors. The guide can be found on Eindhoven’s wiki: https://2015.igem.org/Team:TU_Eindhoven/Collaborations/CloningGuide

You can learn more by clicking on the image on the right.

Manchester-Graz team

The Manchester-Graz team are also working on a project centred around the gut microbiome. We learned this because our PhD adviser, Sibyl, met their supervisor at a carbohydrate conference in May 2015.

Manchester-Graz have developed an expression system designed to regulate single and multi-gene pathways for an intestine expression. They helped us to model butyrate production in the gut. We exchanged parts to conduct reciprocal testing experiments but have so far not been successful in collecting data.

You can see the butyrate production model by clicking on the image on the right.