Difference between revisions of "Team:Cambridge-JIC/Make Your Own js"
| Line 131: | Line 131: | ||
final = function(options) { | final = function(options) { | ||
window.location.href="#CC0"; | window.location.href="#CC0"; | ||
| − | ret = '<h3>Thanks!</h3><br/><h4><strong>Here\'s what you\'ll need\:</strong></h4><br/><table class="table table-striped"><thead><tr><th colspan="2" style="text-align:center">3D PRINTED COMPONENTS</th></tr><tr><th>#</th><th>Part</th></tr></thead><tbody><tr><td>1</td><td>main stage</td></tr><tr><td>1</td><td>base</td></tr><tr><td>2</td><td>side supports for z axis</td></tr><tr><td>1</td><td>cube holder</td></tr><tr><td>1</td><td>z axis in 2 parts (vertical + horizontal)</td></tr><tr><td>1</td><td>3-legged camera holder</td></tr><tr><td>2</td><td>slide clips</td></tr><tr><td>1</td><td>bright-field cube</td></tr><td>1</td><td> | + | ret = '<h3>Thanks!</h3><br/><h4><strong>Here\'s what you\'ll need\:</strong></h4><br/><table class="table table-striped"><thead><tr><th colspan="2" style="text-align:center">3D PRINTED COMPONENTS</th></tr><tr><th>#</th><th>Part</th></tr></thead><tbody><tr><td>1</td><td>main stage</td></tr><tr><td>1</td><td>base</td></tr><tr><td>2</td><td>side supports for z axis</td></tr><tr><td>1</td><td>cube holder</td></tr><tr><td>1</td><td>z axis in 2 parts (vertical + horizontal)</td></tr><tr><td>1</td><td>3-legged camera holder</td></tr><tr><td>2</td><td>slide clips</td></tr><tr><td>1</td><td>bright-field cube (for RPi lens)</td></tr><td>1</td><td>bright-field cube (for ball lens)</td></tr>' |
if(options[1] == 0) { | if(options[1] == 0) { | ||
ret += '<tr><td>1</td><td>dark-field tube in 3 parts</td></tr>' | ret += '<tr><td>1</td><td>dark-field tube in 3 parts</td></tr>' | ||
} | } | ||
if(options[2] == 0 && options[3] == 0 && options[4] == 0) { | if(options[2] == 0 && options[3] == 0 && options[4] == 0) { | ||
| − | ret += '<tr><td>3</td><td>epicubes</td></tr><tr><td>3</td><td>sets of drawers (for filters and mirrors)</td></ | + | ret += '<tr><td>3</td><td>sets of 2 epicubes (1 for RPi lens and 1 for ball lens)</td></tr><tr><td>3</td><td>sets of drawers (for filters and mirrors)</td></tr>' |
} | } | ||
else if((options[2] == 0 && options[3] == 0) || (options[2] == 0 && options[4] == 0) || (options[3] == 0 && options[4] == 0)) { | else if((options[2] == 0 && options[3] == 0) || (options[2] == 0 && options[4] == 0) || (options[3] == 0 && options[4] == 0)) { | ||
| − | ret += '<tr><td>2</td><td>epicubes</td></tr><tr><td>2</td><td>sets of drawers (for filters and mirrors)</td></ | + | ret += '<tr><td>2</td><td>sets of 2 epicubes (1 for RPi lens and 1 for ball lens)</td></tr><tr><td>2</td><td>sets of drawers (for filters and mirrors)</td></tr>' |
} | } | ||
else if(options[2] == 0 || options[3] == 0 || options[4] == 0) { | else if(options[2] == 0 || options[3] == 0 || options[4] == 0) { | ||
| − | ret += '<tr><td>1</td><td> | + | ret += '<tr><td>1</td><td>set of 2 epicubes (1 for RPi lens and 1 for ball lens)</td></tr><tr><td>1</td><td>set of drawers (for filters and mirrors)</td></tr>' |
} | } | ||
| − | |||
| − | |||
| − | |||
if(options[5] == 0 || options[5] == 2) { | if(options[5] == 0 || options[5] == 2) { | ||
ret += '<tr><td>3</td><td>screw gears</td></tr><tr><td>3</td><td>manual gears</td></tr><tr><td>3</td><td>screw holders</td></tr>' | ret += '<tr><td>3</td><td>screw gears</td></tr><tr><td>3</td><td>manual gears</td></tr><tr><td>3</td><td>screw holders</td></tr>' | ||
| Line 205: | Line 202: | ||
ret += '</tbody></table>' | ret += '</tbody></table>' | ||
| − | ret += '<br/>< | + | ret += '<br/><h5><strong>Important notes:</strong></h5><br/><ul>' |
if(options[3] == 0) { | if(options[3] == 0) { | ||
ret += '<li>We haven\'t managed to get RFP imaging working yet so the items marked with an asterisk <strong>(*)</strong> are provided for guidance only\! You may need to find alternative LEDs, filters and dichroic mirror in order to achieve RFP fluorescence.</li><br/>' | ret += '<li>We haven\'t managed to get RFP imaging working yet so the items marked with an asterisk <strong>(*)</strong> are provided for guidance only\! You may need to find alternative LEDs, filters and dichroic mirror in order to achieve RFP fluorescence.</li><br/>' | ||
| Line 214: | Line 211: | ||
ret += '<li>The filters and mirrors are larger than what is needed for a single epicube: if cut carefully into WxD rectangles, a single mirror or filter can be used for N epicubes.</li><br/><li>Only 1 LED is needed per epicube, but we recommend purchasing a few so as to have spares.</li><br/><li>We recommend purchasing 1 RPi camera for each imaging mode (e.g. if you want to image bright-field + GFP + RFP, buy 3 cameras). This is so that you can fix each camera to one cube and leave it there. You CAN buy a single camera for several imaging modes and swap it between cubes, but this will increase the risk of damaging the CCD.</li></ul>' | ret += '<li>The filters and mirrors are larger than what is needed for a single epicube: if cut carefully into WxD rectangles, a single mirror or filter can be used for N epicubes.</li><br/><li>Only 1 LED is needed per epicube, but we recommend purchasing a few so as to have spares.</li><br/><li>We recommend purchasing 1 RPi camera for each imaging mode (e.g. if you want to image bright-field + GFP + RFP, buy 3 cameras). This is so that you can fix each camera to one cube and leave it there. You CAN buy a single camera for several imaging modes and swap it between cubes, but this will increase the risk of damaging the CCD.</li></ul>' | ||
| − | ret += '<br/><h4><strong>Assembly instructions:</strong></h4><br/>< | + | ret += '<br/><h4><strong>Assembly instructions:</strong></h4><br/><ul><li>Make sure you have some superglue, blue-tak, sticky tape, a file and some pliers. Print all the necessary parts. You'll notice that the printer adds a sort of brim to the bottom surface of each part. Cut away the brim for each of the parts, and file the edges if necessary.</li><li>Remove the lens from the Raspberry Pi camera. Using a pair of pliers (the kind with a ridged surface), grip the top circular plastic part of the lens and rotate counter-clockwise. It may be easier to grip the lens firmly and rotate the sensor, rather than the other way around. Once the lens in fully unscrewed, you can just pull it out. Quickly cover the resulting hole with some sticky tape to protect the CCD from dust particles.</li><br/><li>Take the lens you just removed from the RPi camera and push it into the bright-field cube for RPi lens (into the round hole). Make sure that the larger side of the lens is facing outwards.</li><br/><li>Whenever you need better resolution, use the ball lens instead of the RPi lens (keep in mind it will have smaller field of view!). In that case, use the bright-field cube for ball lens. Push the ball lens into the round hole. You can pop the lens in and out if needed, but be careful not to damage it!</li><br/><li>Let's assemble the chassis of the microscope. Using superglue (we recommend Loctite\; apply with a brush) for the following steps:<ul><li>glue 2 nuts on the bottom of the main stage, into the nut-shaped holes</li><li>glue 1 nut on the bottom of the z axis, into the nut-shaped hole (the z axis comes in two parts - the nut goes on the part shaped like a square with a corner missing)</li><li>glue the base onto the bottom the main stage</li><li>screw caps (x3)</li>' |
if(options[5] == 0) { | if(options[5] == 0) { | ||
ret += '<li>screw holders (x3)</li>' | ret += '<li>screw holders (x3)</li>' | ||
| Line 231: | Line 228: | ||
ret += '<li>To use the microscope in motorised mode, add the motors as follows: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors.</li><br/>' | ret += '<li>To use the microscope in motorised mode, add the motors as follows: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors.</li><br/>' | ||
} | } | ||
| − | ret +='</ | + | ret +='</ul>' |
return $('<div>').html(ret) | return $('<div>').html(ret) | ||
Revision as of 18:15, 17 September 2015
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$(document).ready(function(){
q = new quiz('#cam-quiz');
step0 = new q.step("Tell us which options you'd like for your microscope.", {
1: "Okay!"
});
step1 = new q.step("Besides bright-field, do you need dark-field imaging?", {
0: "Yes",
1: "No"
});
step2 = new q.step("Do you need to image GFP fluorescence?", {
0: "Yes",
1: "No"
});
step3 = new q.step("Do you need to image RFP fluorescence?", {
0: "Yes",
1: "No"
});
step4 = new q.step("Do you need to image another fluorescent protein, e.g. YFP?", {
0: "Yes",
1: "No"
});
step5 = new q.step("Do you want your microscope to be manual, motorised, or will you need to swap between both?", {
0: "Manual only",
1: "Motorised only",
2: "Both",
});
step6 = new q.step("Do you need your microscope to be fully battery-powered, e.g. for fieldwork?", {
0: "Yes",
1: "No"
});
final = function(options) {
window.location.href="#CC0";
ret = '<h3>Thanks!</h3>
Here\'s what you\'ll need\:
<thead>3D PRINTED COMPONENTS # Part 1 main stage 1 base 2 side supports for z axis 1 cube holder 1 z axis in 2 parts (vertical + horizontal) 1 3-legged camera holder 2 slide clips 1 bright-field cube (for RPi lens) 1 bright-field cube (for ball lens) </tr>'
if(options[1] == 0) {
ret += '1 dark-field tube in 3 parts }
if(options[2] == 0 && options[3] == 0 && options[4] == 0) {
ret += '3 sets of 2 epicubes (1 for RPi lens and 1 for ball lens) 3 sets of drawers (for filters and mirrors) }
else if((options[2] == 0 && options[3] == 0) || (options[2] == 0 && options[4] == 0) || (options[3] == 0 && options[4] == 0)) {
ret += '2 sets of 2 epicubes (1 for RPi lens and 1 for ball lens) 2 sets of drawers (for filters and mirrors) }
else if(options[2] == 0 || options[3] == 0 || options[4] == 0) {
ret += '1 set of 2 epicubes (1 for RPi lens and 1 for ball lens) 1 set of drawers (for filters and mirrors) }
if(options[5] == 0 || options[5] == 2) {
ret += '3 screw gears 3 manual gears 3 screw holders }
if(options[5] == 1 || options[5] == 2) {
ret += '3 screw gears for motors 3 motor holders }
if((options[5] == 1 || options[5] == 2) && options[6] == 1) {
ret += '1 battery holder for RPi }
if((options[5] == 1 || options[5] == 2) && options[6] == 0) {
ret += '2 battery holders for RPi }
if(options[6] == 0 && !(options[5] == 1 || options[5] == 2)) {
ret += '1 battery holder for RPi }
ret += '</tbody>
'
ret += 'All 3D printed components can be found in this ZIP file [LINK TO ZIP FOLDER WITH ALL SCAD & STL FILES]. For each of these parts, STL and SCAD files are provided. Use the STL file if you want to view the component, and the SCAD file if you need to edit it (using OpenSCAD or similar software).
However, when it comes to making the microscope, do print several components in one go - it will save some time. Just print everything that is in this file [INCLUDE COMBINED PRINT FILES]. And make sure to check out our 3D printing tips [LINK TO SO YOU THINK YOU CAN PRINT]
If you do not have access to a 3D printer, you can send your files to a 3D printing service and they’ll ship your printed parts.
'
ret += '
<thead>OFF-THE-SHELF COMPONENTS # Part Cost/unit (GBP) Details & link to provider 1 white LED (bright-field) 0.554 <a href="http://uk.rs-online.com/web/p/visible-leds/7133955/?searchTerm=NSPW500DS&relevancy-data=636F3D3226696E3D4931384E4B6E6F776E41734D504E266C753D656E266D6D3D6D61746368616C6C7061727469616C26706D3D5E5B5C707B4C7D5C707B4E647D2D2C2F255C2E5D2B2426706F3D313326736E3D592673743D4D414E5F504152545F4E554D4245522677633D424F5448267573743D4E535057353030445326" style="color:#0000bb">Mfr: Nichia; Part No. NSPW500DS</a> (must be bought in multiples of 5) 1 ball lens 12.20 <a href="http://www.comaroptics.com/components/lenses/simple-convex-lenses/sapphire-and-glass-ball-lenses#row-03_vq_04" style="color:#0000bb">Mfr: Comar; Part No: 03 VQ 04</a> 2 25 mm long M4 screws 0.107 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-25mm-blister-10-msph425b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> 1 40 mm long M4 screw 0.107 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-40mm-blister-6-msph440b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> 3 M4 nuts 0.0043 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-hexagon-nut-zp-m4-bag-100-100nut4-hexagonal-nuts-bolts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> 3 M4 washers 0.0037 Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-flat-washer-heavyduty-zp-m4-bag-100-100hdwash4-washers-bolts-nuts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> 1 Raspberry Pi 2 Model B 26.60 <a href="http://uk.rs-online.com/web/p/processor-microcontroller-development-kits/832-6274/" style="color:#0000bb">Processor part number: BCM2836</a> 1 Ralink RT5370 WiFi adapter 9.10 <a href="http://www.amazon.co.uk/gp/product/B00IQFGJSS?psc=1&redirect=true&ref_=oh_aui_detailpage_o02_s00" style="color:#0000bb">Amazon Standard Identification Number (ASIN)\: B00IQFGJSS</a> 1 Arduino UNO with its USB B connector 21.00 <a href="https://shop.pimoroni.com/products/arduino-uno" style="color:#0000bb">Mfr: Arduino; Part No. A000066</a> 1 breadboard 2.82 <a href="http://uk.farnell.com/multicomp/mcbb400/breadboard-solderless-300v-abs/dp/2395961?ost=2395961&mckv=ezr2Yisy&CMP=KNC-GUK-FUK-GEN-SHOPPING-MULTICOMP&CAGPSPN=pla&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaEPR46s_SrENpnwFOv5PS_hqUNVzk8VV5QPxB9AZBwYaAupo8P8HAQ&CAWELAID=120173390000249291&gross_price=true" style="color:#0000bb">Mfr: Multicomp; Part No. MCBB400</a> 1 16GB micro SD card 5.43 <a href="http://www.amazon.co.uk/SanDisk-Ultra-Imaging-C10-MicroSDHC/dp/B00MBTP8Q4/ref=sr_1_6?ie=UTF8&qid=1442328635&sr=8-6&keywords=micro+sd+16gb+memory+cards" style="color:#0000bb">Model number: SDSDQUIN-016G-G4; ASIN: B00MBTP8Q4</a> 2 220 ohm resistors 0.065 <a href="http://uk.rs-online.com/web/p/through-hole-fixed-resistors/0131794/" style="color:#0000bb">Brand: TE Connectivity; Part No. CFR100J220R</a> (must be bought in multiples of 10) 1 ethernet cable 9.99 <a href="http://www.pcworld.co.uk/gbuk/computing-accessories/computer-accessories/power-and-cables/computing-cables-and-adaptors/sandstrom-scat65m12-rj45-to-rj45-cat-6-ethernet-cable-5m-12567142-pdt.html" style="color:#0000bb">Mfr: Sandstrøm; Product code: 874926</a> 1 100 m spool insulated single-core wire 13.45 <a href="http://uk.rs-online.com/web/p/single-core-control-cable/7244311/" style="color:#0000bb">Brand: Lapp; Part No. 4510021</a> if(options[2] == 0 && options[3] == 0 && options[4] == 0) {
ret += '4 RPi cameras 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera '
}
else if((options[2] == 0 && options[3] == 0) || (options[2] == 0 && options[4] == 0) || (options[3] == 0 && options[4] == 0)) {
ret += '3 RPi cameras 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera } else if(options[2] == 0 || options[3] == 0 || options[4] == 0) {
ret += '2 RPi cameras 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera }
else {
ret +='1 RPi camera 22.18 <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> 1 RPi ribbon cable 0.00 Comes with RPi camera }
if(options[2] == 0) {
ret += '1 blue LED (GFP) 0.225 <a href="http://onecall.farnell.com/kingbright/l-10934vbc-ds-d/led-3mm-blue-4000mcd-470nm/dp/2373492?ost=2373492" style="color:#0000bb">Mfr: Kingbright; Part No. L-10934VBC/DS-D</a> 1 550nm dichroic mirror, 25x16mm (GFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters#row-550_iy_116" style="color:#0000bb">Mfr: Comar; Part No. 550 1Y 116</a> 1 490nm excitation filter, 25x16mm (GFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/short-pass-dichroic-filters#row-495_ik_116" style="color:#0000bb">Mfr: Comar; Part No. 495 1K 116</a> 1 500nm emission filter, 25x16mm (GFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/band-pass-dichroic-filters#row-515_ib_116" style="color:#0000bb">Mfr: Comar; Part No. 515 1B 116</a> }
if(options[3] == 0) {
ret += '1 amber LED* (RFP) 1.088 <a href="http://uk.rs-online.com/web/p/visible-leds/8107834/" style="color:#0000bb">Brand: OSRAM Opto Semiconductors; Part No. LY CKBP-JYKX-36-1</a> (must be bought in multiples of 5) 1 dichroic mirror* (RFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters#row-650_iy_116" style="color:#0000bb">Mfr: Comar; Part No. 650 IY 116</a> 1 excitation filter* (RFP) 19.40 <a href="http://www.comaroptics.com/components/filters/dichroic-filters/band-pass-dichroic-filters#row-534_ib_116" style="color:#0000bb">Mfr: Comar; Part No. 534 IB 116</a> 1 emission filter* (RFP) 19.40 <a href="http://www.comaroptics.com/components/filters/gelatin-and-polyester-colour-filters#row-575_wb_75" style="color:#0000bb">Mfr: Comar; Part No. 575 WB 75</a> }
if(options[4] == 0) {
ret += '5 LEDs** ? tailored to FP 1 dichroic mirror** ? tailored to FP 1 excitation filter** ? tailored to FP 1 emission filter** ? tailored to FP }
if(options[5] == 1 || options[5] == 2) {
ret += '3 stepper motors 3.95 <a href="http://onecall.farnell.com/adafruit-industries/858/stepper-motor-512-step-5vdc/dp/MC02058?ost=MC02058" style="color:#0000bb">Mfr: Adafruit Industries; Part No. 858</a> 2 UNL2003A Darlington driver chips 0.42 <a href="http://uk.rs-online.com/web/p/darlington-transistors/4368451/" style="color:#0000bb">Mfr: Texas Instruments; Part No. ULN2003AN</a> }
if((options[5] == 1 || options[5] == 2) && options[6] == 1) {
ret += '1 9V battery connector 0.172 <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> }
if((options[5] == 1 || options[5] == 2) && options[6] == 0) {
ret += '2 9V battery connectors 0.172 <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> }
if(options[6] == 0 && !(options[5] == 1 || options[5] == 2)) {
ret += '1 9V battery connector 0.172 <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> }
if(options[6] == 0) {
ret += '1 1 Low Profile MoPi power board 25.00 <a href="https://shop.pimoroni.com/products/mopi-mobile-pi-power?utm_medium=cpc&utm_source=googlepla&variant=909042229&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaMQfkAkb65Xgv6GXoWhTroobFXImuYP392yZocnZEJIaAoYM8P8HAQ" style="color:#0000bb">Product code: MOPI-LP</a> }
if(options[6] == 1) {
ret += '1 2A micro USB power cable 5.66 <a href="http://uk.rs-online.com/web/p/plug-in-power-supply/7653311/" style="color:#0000bb">Mfr: RS; Part No. HNP10I-microUSB</a> }
ret += '</tbody>
'
ret += '
Important notes:
'
if(options[3] == 0) {
ret += 'We haven\'t managed to get RFP imaging working yet so the items marked with an asterisk (*) are provided for guidance only\! You may need to find alternative LEDs, filters and dichroic mirror in order to achieve RFP fluorescence.
'
}
if(options[4] == 0) {
ret += 'You are looking to image another fluorescent protein e.g. YFP. You will need to find adequate LEDs, filters and dichroic mirror - these items are marked with two asterisks (**). Otherwise, just follow instructions as usual.
'
}
ret += 'The filters and mirrors are larger than what is needed for a single epicube: if cut carefully into WxD rectangles, a single mirror or filter can be used for N epicubes.
Only 1 LED is needed per epicube, but we recommend purchasing a few so as to have spares.
We recommend purchasing 1 RPi camera for each imaging mode (e.g. if you want to image bright-field + GFP + RFP, buy 3 cameras). This is so that you can fix each camera to one cube and leave it there. You CAN buy a single camera for several imaging modes and swap it between cubes, but this will increase the risk of damaging the CCD. '
ret += '
Assembly instructions:
- Make sure you have some superglue, blue-tak, sticky tape, a file and some pliers. Print all the necessary parts. You'll notice that the printer adds a sort of brim to the bottom surface of each part. Cut away the brim for each of the parts, and file the edges if necessary.
- Remove the lens from the Raspberry Pi camera. Using a pair of pliers (the kind with a ridged surface), grip the top circular plastic part of the lens and rotate counter-clockwise. It may be easier to grip the lens firmly and rotate the sensor, rather than the other way around. Once the lens in fully unscrewed, you can just pull it out. Quickly cover the resulting hole with some sticky tape to protect the CCD from dust particles.
- Take the lens you just removed from the RPi camera and push it into the bright-field cube for RPi lens (into the round hole). Make sure that the larger side of the lens is facing outwards.
- Whenever you need better resolution, use the ball lens instead of the RPi lens (keep in mind it will have smaller field of view!). In that case, use the bright-field cube for ball lens. Push the ball lens into the round hole. You can pop the lens in and out if needed, but be careful not to damage it!
- Let's assemble the chassis of the microscope. Using superglue (we recommend Loctite\; apply with a brush) for the following steps:
- glue 2 nuts on the bottom of the main stage, into the nut-shaped holes
- glue 1 nut on the bottom of the z axis, into the nut-shaped hole (the z axis comes in two parts - the nut goes on the part shaped like a square with a corner missing)
- glue the base onto the bottom the main stage
- screw caps (x3)
'
if(options[5] == 0) {
ret += ' - screw holders (x3)
'
}
if(options[5] == 1) {
ret += 'motor holders (x3) '
}
if(options[5] == 2) {
ret += 'motor holders (x3) or screw holders (x3) depending on whether motorised or manual mode is used '
}
ret += 'the 2 parts of the z axis together RPi lens into lens holder supporting axes (x2) into cube holder washer into large leg (around the hole)
Fit the following parts in (you might have to file all holes until they are smooth and large enough...)\:- slide the main z axis into its slot (the central one) from the bottom, make sure it can move smoothly
- slide the remaining z axis assembly in from top
- make sure the main z axis fits into the slot in the camera holder, if it does - glue it in place
- slide the lens (with its holder) into the camera cube
- click the camera cube into the square hole of the camera holder, make sure it goes all the way down (front face of cube at the level of the bottom surface of the holder)
- detach the camera sensor from the camera board, click the RPi Cam sensor into the camera cube, click the SUNNY back to the board (make sure it stays connected at all times!!!)
- put screws into holes on the top cover, place a washer under each screwtop and screw into the nuts (make sure the superglue has dried...) - now you can move the stage and the z axis and you are ready to image!
'
if(options[5] == 1) {
ret += 'To add the motors: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors
'
}
if(options[5] == 2) {
ret += 'To use the microscope in motorised mode, add the motors as follows: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors.
'
}
ret +=''
return $('').html(ret)
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step0.bind(step1, [1]);
step1.bind(step2, [0]);
step1.bind(step2, [1]);
step2.bind(step3, [0]);
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step3.bind(step4, [1]);
step4.bind(step5, [0]);
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step5.bind(step6, [0]);
step5.bind(step6, [1]);
step5.bind(step6, [2]);
step6.bind(final, [0]);
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q.start(step0);
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.text(this.answers[key].answer)
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ref.elem.find('.cam-quiz-selected').removeClass('cam-quiz-selected');
$(this).addClass('cam-quiz-selected');
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var nref = {
'step': undefined,
'elem': $('')
Here\'s what you\'ll need\:
<thead>
'
<thead>
'
ret += '
We haven\'t managed to get RFP imaging working yet so the items marked with an asterisk (*) are provided for guidance only\! You may need to find alternative LEDs, filters and dichroic mirror in order to achieve RFP fluorescence.
'You are looking to image another fluorescent protein e.g. YFP. You will need to find adequate LEDs, filters and dichroic mirror - these items are marked with two asterisks (**). Otherwise, just follow instructions as usual.
'The filters and mirrors are larger than what is needed for a single epicube: if cut carefully into WxD rectangles, a single mirror or filter can be used for N epicubes.
Only 1 LED is needed per epicube, but we recommend purchasing a few so as to have spares.
We recommend purchasing 1 RPi camera for each imaging mode (e.g. if you want to image bright-field + GFP + RFP, buy 3 cameras). This is so that you can fix each camera to one cube and leave it there. You CAN buy a single camera for several imaging modes and swap it between cubes, but this will increase the risk of damaging the CCD. '
ret += '
motor holders (x3) '
motor holders (x3) or screw holders (x3) depending on whether motorised or manual mode is used '
the 2 parts of the z axis together RPi lens into lens holder supporting axes (x2) into cube holder washer into large leg (around the hole)
Fit the following parts in (you might have to file all holes until they are smooth and large enough...)\:
'To add the motors: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors
'To use the microscope in motorised mode, add the motors as follows: place the camshaft of the motor into the hole designated for it (in the screw cap) and click the motor in - the body should now touch the holder surface, press hard so that it glues in...repeat for all three motors.
'
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'option': undefined
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quiz.steps.push(nref);
quiz.elem.append(nref.elem);
break;
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alert("Quiz error! Please contact the site maintainers....");
break;
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return elem;
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answer = answers[key];
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'elem': step.elem(this, index),
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this.steps.push(ref);
this.elem.append(ref.elem);
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$(document).ready(function(){
q = new quiz('#cam-quiz');
step0 = new q.step("Tell us which options you'd like for your microscope.", {
1: "Okay!"
});
step1 = new q.step("Besides bright-field, do you need dark-field imaging?", {
0: "Yes",
1: "No"
});
step2 = new q.step("Do you need to image GFP fluorescence?", {
0: "Yes",
1: "No"
});
step3 = new q.step("Do you need to image RFP fluorescence?", {
0: "Yes",
1: "No"
});
step4 = new q.step("Do you need to image another fluorescent protein, e.g. YFP?", {
0: "Yes",
1: "No"
});
step5 = new q.step("Do you want your microscope to be manual, motorised, or will you need to swap between both?", {
0: "Manual only",
1: "Motorised only",
2: "Both",
});
step6 = new q.step("Do you need your microscope to be fully battery-powered, e.g. for fieldwork?", {
0: "Yes",
1: "No"
});
final = function(options) {
window.location.href="#CC0";ret = '<h3>Thanks!</h3>
Here\'s what you\'ll need\:
| 3D PRINTED COMPONENTS | |
|---|---|
| # | Part |
| 1 | main stage |
| 1 | base |
| 2 | side supports for z axis |
| 1 | cube holder |
| 1 | z axis in 2 parts (vertical + horizontal) |
| 1 | 3-legged camera holder |
| 2 | slide clips |
| 1 | bright-field cube (for RPi lens) | 1 | bright-field cube (for ball lens) | </tr>'
| 1 | dark-field tube in 3 parts |
| 3 | sets of 2 epicubes (1 for RPi lens and 1 for ball lens) |
| 3 | sets of drawers (for filters and mirrors) |
| 2 | sets of 2 epicubes (1 for RPi lens and 1 for ball lens) |
| 2 | sets of drawers (for filters and mirrors) |
| 1 | set of 2 epicubes (1 for RPi lens and 1 for ball lens) |
| 1 | set of drawers (for filters and mirrors) |
| 3 | screw gears |
| 3 | manual gears |
| 3 | screw holders |
| 3 | screw gears for motors |
| 3 | motor holders |
| 1 | battery holder for RPi |
| 2 | battery holders for RPi |
| 1 | battery holder for RPi |
ret += 'All 3D printed components can be found in this ZIP file [LINK TO ZIP FOLDER WITH ALL SCAD & STL FILES]. For each of these parts, STL and SCAD files are provided. Use the STL file if you want to view the component, and the SCAD file if you need to edit it (using OpenSCAD or similar software).
However, when it comes to making the microscope, do print several components in one go - it will save some time. Just print everything that is in this file [INCLUDE COMBINED PRINT FILES]. And make sure to check out our 3D printing tips [LINK TO SO YOU THINK YOU CAN PRINT]
If you do not have access to a 3D printer, you can send your files to a 3D printing service and they’ll ship your printed parts.
'
| OFF-THE-SHELF COMPONENTS | |||
|---|---|---|---|
| # | Part | Cost/unit (GBP) | Details & link to provider |
| 1 | white LED (bright-field) | 0.554 | <a href="http://uk.rs-online.com/web/p/visible-leds/7133955/?searchTerm=NSPW500DS&relevancy-data=636F3D3226696E3D4931384E4B6E6F776E41734D504E266C753D656E266D6D3D6D61746368616C6C7061727469616C26706D3D5E5B5C707B4C7D5C707B4E647D2D2C2F255C2E5D2B2426706F3D313326736E3D592673743D4D414E5F504152545F4E554D4245522677633D424F5448267573743D4E535057353030445326" style="color:#0000bb">Mfr: Nichia; Part No. NSPW500DS</a> (must be bought in multiples of 5) |
| 1 | ball lens | 12.20 | <a href="http://www.comaroptics.com/components/lenses/simple-convex-lenses/sapphire-and-glass-ball-lenses#row-03_vq_04" style="color:#0000bb">Mfr: Comar; Part No: 03 VQ 04</a> |
| 2 | 25 mm long M4 screws | 0.107 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-25mm-blister-10-msph425b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> |
| 1 | 40 mm long M4 screw | 0.107 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-machine-screw-pozi-pan-head-zp-m4-40mm-blister-6-msph440b-screws-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 10)</a> |
| 3 | M4 nuts | 0.0043 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-hexagon-nut-zp-m4-bag-100-100nut4-hexagonal-nuts-bolts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> |
| 3 | M4 washers | 0.0037 | Buy at hardware store<a ref="https://www.mackay.co.uk/forgefix-flat-washer-heavyduty-zp-m4-bag-100-100hdwash4-washers-bolts-nuts-fixings-hardware-security-toolbank-brands.html" style="color:#0000bb"> (reference price/unit for a pack of 100)</a> |
| 1 | Raspberry Pi 2 Model B | 26.60 | <a href="http://uk.rs-online.com/web/p/processor-microcontroller-development-kits/832-6274/" style="color:#0000bb">Processor part number: BCM2836</a> |
| 1 | Ralink RT5370 WiFi adapter | 9.10 | <a href="http://www.amazon.co.uk/gp/product/B00IQFGJSS?psc=1&redirect=true&ref_=oh_aui_detailpage_o02_s00" style="color:#0000bb">Amazon Standard Identification Number (ASIN)\: B00IQFGJSS</a> |
| 1 | Arduino UNO with its USB B connector | 21.00 | <a href="https://shop.pimoroni.com/products/arduino-uno" style="color:#0000bb">Mfr: Arduino; Part No. A000066</a> |
| 1 | breadboard | 2.82 | <a href="http://uk.farnell.com/multicomp/mcbb400/breadboard-solderless-300v-abs/dp/2395961?ost=2395961&mckv=ezr2Yisy&CMP=KNC-GUK-FUK-GEN-SHOPPING-MULTICOMP&CAGPSPN=pla&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaEPR46s_SrENpnwFOv5PS_hqUNVzk8VV5QPxB9AZBwYaAupo8P8HAQ&CAWELAID=120173390000249291&gross_price=true" style="color:#0000bb">Mfr: Multicomp; Part No. MCBB400</a> |
| 1 | 16GB micro SD card | 5.43 | <a href="http://www.amazon.co.uk/SanDisk-Ultra-Imaging-C10-MicroSDHC/dp/B00MBTP8Q4/ref=sr_1_6?ie=UTF8&qid=1442328635&sr=8-6&keywords=micro+sd+16gb+memory+cards" style="color:#0000bb">Model number: SDSDQUIN-016G-G4; ASIN: B00MBTP8Q4</a> |
| 2 | 220 ohm resistors | 0.065 | <a href="http://uk.rs-online.com/web/p/through-hole-fixed-resistors/0131794/" style="color:#0000bb">Brand: TE Connectivity; Part No. CFR100J220R</a> (must be bought in multiples of 10) |
| 1 | ethernet cable | 9.99 | <a href="http://www.pcworld.co.uk/gbuk/computing-accessories/computer-accessories/power-and-cables/computing-cables-and-adaptors/sandstrom-scat65m12-rj45-to-rj45-cat-6-ethernet-cable-5m-12567142-pdt.html" style="color:#0000bb">Mfr: Sandstrøm; Product code: 874926</a> |
| 1 | 100 m spool insulated single-core wire | 13.45 | <a href="http://uk.rs-online.com/web/p/single-core-control-cable/7244311/" style="color:#0000bb">Brand: Lapp; Part No. 4510021</a> |
| 4 | RPi cameras | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera | '
| 3 | RPi cameras | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera |
| 2 | RPi cameras | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera |
| 1 | RPi camera | 22.18 | <a href="http://www.misco.co.uk/product/217272/Raspberry-Pi-NoIR-Camera-Board?fromProductList=Search+result" style="color:#0000bb">Brand: Raspberry Pi; Part No. PiNoir</a> |
| 1 | RPi ribbon cable | 0.00 | Comes with RPi camera |
| 1 | blue LED (GFP) | 0.225 | <a href="http://onecall.farnell.com/kingbright/l-10934vbc-ds-d/led-3mm-blue-4000mcd-470nm/dp/2373492?ost=2373492" style="color:#0000bb">Mfr: Kingbright; Part No. L-10934VBC/DS-D</a> |
| 1 | 550nm dichroic mirror, 25x16mm (GFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters#row-550_iy_116" style="color:#0000bb">Mfr: Comar; Part No. 550 1Y 116</a> |
| 1 | 490nm excitation filter, 25x16mm (GFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/short-pass-dichroic-filters#row-495_ik_116" style="color:#0000bb">Mfr: Comar; Part No. 495 1K 116</a> |
| 1 | 500nm emission filter, 25x16mm (GFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/band-pass-dichroic-filters#row-515_ib_116" style="color:#0000bb">Mfr: Comar; Part No. 515 1B 116</a> |
| 1 | amber LED* (RFP) | 1.088 | <a href="http://uk.rs-online.com/web/p/visible-leds/8107834/" style="color:#0000bb">Brand: OSRAM Opto Semiconductors; Part No. LY CKBP-JYKX-36-1</a> (must be bought in multiples of 5) |
| 1 | dichroic mirror* (RFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/long-pass-dichroic-filters#row-650_iy_116" style="color:#0000bb">Mfr: Comar; Part No. 650 IY 116</a> |
| 1 | excitation filter* (RFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/dichroic-filters/band-pass-dichroic-filters#row-534_ib_116" style="color:#0000bb">Mfr: Comar; Part No. 534 IB 116</a> |
| 1 | emission filter* (RFP) | 19.40 | <a href="http://www.comaroptics.com/components/filters/gelatin-and-polyester-colour-filters#row-575_wb_75" style="color:#0000bb">Mfr: Comar; Part No. 575 WB 75</a> |
| 5 | LEDs** | ? | tailored to FP |
| 1 | dichroic mirror** | ? | tailored to FP |
| 1 | excitation filter** | ? | tailored to FP |
| 1 | emission filter** | ? | tailored to FP |
| 3 | stepper motors | 3.95 | <a href="http://onecall.farnell.com/adafruit-industries/858/stepper-motor-512-step-5vdc/dp/MC02058?ost=MC02058" style="color:#0000bb">Mfr: Adafruit Industries; Part No. 858</a> |
| 2 | UNL2003A Darlington driver chips | 0.42 | <a href="http://uk.rs-online.com/web/p/darlington-transistors/4368451/" style="color:#0000bb">Mfr: Texas Instruments; Part No. ULN2003AN</a> |
| 1 | 9V battery connector | 0.172 | <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> |
| 2 | 9V battery connectors | 0.172 | <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> |
| 1 | 9V battery connector | 0.172 | <a href="http://uk.rs-online.com/web/p/battery-holders-mounts/0489021/" style="color:#0000bb">Brand: RS; Stock No. 489-021</a> |
| 1 | 1 Low Profile MoPi power board | 25.00 | <a href="https://shop.pimoroni.com/products/mopi-mobile-pi-power?utm_medium=cpc&utm_source=googlepla&variant=909042229&gclid=Cj0KEQjwg9-vBRCK7L7wmO2u0JcBEiQA_tzoaMQfkAkb65Xgv6GXoWhTroobFXImuYP392yZocnZEJIaAoYM8P8HAQ" style="color:#0000bb">Product code: MOPI-LP</a> |
| 1 | 2A micro USB power cable | 5.66 | <a href="http://uk.rs-online.com/web/p/plug-in-power-supply/7653311/" style="color:#0000bb">Mfr: RS; Part No. HNP10I-microUSB</a> |
Important notes:
- '
if(options[3] == 0) {
ret += ''
}
if(options[4] == 0) {
ret += ''
}ret += '
Assembly instructions:
- Make sure you have some superglue, blue-tak, sticky tape, a file and some pliers. Print all the necessary parts. You'll notice that the printer adds a sort of brim to the bottom surface of each part. Cut away the brim for each of the parts, and file the edges if necessary.
- Remove the lens from the Raspberry Pi camera. Using a pair of pliers (the kind with a ridged surface), grip the top circular plastic part of the lens and rotate counter-clockwise. It may be easier to grip the lens firmly and rotate the sensor, rather than the other way around. Once the lens in fully unscrewed, you can just pull it out. Quickly cover the resulting hole with some sticky tape to protect the CCD from dust particles.
- Take the lens you just removed from the RPi camera and push it into the bright-field cube for RPi lens (into the round hole). Make sure that the larger side of the lens is facing outwards.
- Whenever you need better resolution, use the ball lens instead of the RPi lens (keep in mind it will have smaller field of view!). In that case, use the bright-field cube for ball lens. Push the ball lens into the round hole. You can pop the lens in and out if needed, but be careful not to damage it!
- Let's assemble the chassis of the microscope. Using superglue (we recommend Loctite\; apply with a brush) for the following steps:
- glue 2 nuts on the bottom of the main stage, into the nut-shaped holes
- glue 1 nut on the bottom of the z axis, into the nut-shaped hole (the z axis comes in two parts - the nut goes on the part shaped like a square with a corner missing)
- glue the base onto the bottom the main stage
- screw caps (x3) '
if(options[5] == 0) {ret += ' - screw holders (x3) '
}
if(options[5] == 1) {
ret += ' }
if(options[5] == 2) {
ret += '}ret += '
- slide the main z axis into its slot (the central one) from the bottom, make sure it can move smoothly
- slide the remaining z axis assembly in from top
- make sure the main z axis fits into the slot in the camera holder, if it does - glue it in place
- slide the lens (with its holder) into the camera cube
- click the camera cube into the square hole of the camera holder, make sure it goes all the way down (front face of cube at the level of the bottom surface of the holder)
- detach the camera sensor from the camera board, click the RPi Cam sensor into the camera cube, click the SUNNY back to the board (make sure it stays connected at all times!!!)
- put screws into holes on the top cover, place a washer under each screwtop and screw into the nuts (make sure the superglue has dried...) - now you can move the stage and the z axis and you are ready to image!
'
if(options[5] == 1) {
ret += ''
}
if(options[5] == 2) {
ret += ''
}ret +='' return $('
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}
step0.bind(step1, [1]);
step1.bind(step2, [0]);
step1.bind(step2, [1]);
step2.bind(step3, [0]);
step2.bind(step3, [1]);
step3.bind(step4, [0]);
step3.bind(step4, [1]);
step4.bind(step5, [0]);
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step5.bind(step6, [0]);
step5.bind(step6, [1]);
step5.bind(step6, [2]);
step6.bind(final, [0]);
step6.bind(final, [1]);
q.start(step0);
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