Difference between revisions of "Template:Team:Groningen/CONTENT/COLLABORATIONS/Collaborations"

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<div class="subtitle">iGEM Aix Marseile</div>
 
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<div class="text"> We helped the iGEM team of Aix Marseille out with their survey on chewing gum. They have awarded us with a gold medal badge because we have entered more than 30 surveys.  
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<div class="text"> We helped the iGEM team of Aix Marseille out with their survey on chewing gum. They awarded us with a gold medal badge because we submitted more than 30 surveys.  
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The iGEM team of the TU Delft used their 3D printer to create an artificial biofilm of our B. Subtilis 3610 ComI in alginate. We tested this layer in our set up to see if this could be used in our project. The layer was unfortunately not able to block water and we could not measure any selectivity.  
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During the national meetup in the Netherlands, the iGEM team from TU Delft told about their plans of building a 3D biofilm printer. Our project is about an ion-selective biofilm so we decided to collaborate. When the K’NEX 3D printer was ready Anne and Marit from the iGEM Delft team travelled to Groningen to test if they could print with our B. subtilis 3610 comI. It was indeed seen that the printer also worked with B. subtilis, using a mixture of bacteria in alginate, with using calcium chloride for polymerisation. As alginate is negatively charged, we decided to expand the experiment to also test the printed biofilm in our test device to test its selectivity for sodium ions. We tested the printed layer in our setup, but unfortunately the layer was not able to block water, making it impossible to measure a reliable value for the potential.
 
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This iGEM team asked us to sing a part of a iGEM song for them which would be used to create a iGEM song with all the participating teams. The result should be visible on the jamboree.  
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This iGEM team asked us to sing part of their Human Practices iGEM song, which would be used to create a iGEM song with all the participating teams. We submitted the first part of the song! The result should be visible on the Jamboree.  
 
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TecCEM HS asked for our help since they had problems getting their ordered DNA through customs. We ordered their DNA sequence for ferritin at IDT and ligated into the pSB1C3 backbone which we then transformed into E. coli. Their construct has an IPTG inducible promotor which we tested by IPTG induction. The SDS-page gel shows that the protein is expressed when adding IPTG. This confirms that the construct works.  
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TecCEM HS asked for our help since they had problems getting their ordered DNA through customs. The DNA sequence for ferritin was ordered at IDT and ligated into the pSB1C3 backbone which then was transformed into E. coli. Their construct has an IPTG inducible promoter which was tested by IPTG induction. The SDS-page gel has shown that the protein is expressed when adding IPTG. This confirms that the construct works.  
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Picture: Picture shows that after IPTG induction a thick band is visible of approximately 42kD
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<b>Picture: Picture shows that after IPTG induction a thick band is visible of approximately 42kD </b>
 
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Revision as of 19:56, 17 September 2015

iGEM Aix Marseile
We helped the iGEM team of Aix Marseille out with their survey on chewing gum. They awarded us with a gold medal badge because we submitted more than 30 surveys.

iGEM TU Delft

During the national meetup in the Netherlands, the iGEM team from TU Delft told about their plans of building a 3D biofilm printer. Our project is about an ion-selective biofilm so we decided to collaborate. When the K’NEX 3D printer was ready Anne and Marit from the iGEM Delft team travelled to Groningen to test if they could print with our B. subtilis 3610 comI. It was indeed seen that the printer also worked with B. subtilis, using a mixture of bacteria in alginate, with using calcium chloride for polymerisation. As alginate is negatively charged, we decided to expand the experiment to also test the printed biofilm in our test device to test its selectivity for sodium ions. We tested the printed layer in our setup, but unfortunately the layer was not able to block water, making it impossible to measure a reliable value for the potential.

iGEM Linkoping

This iGEM team asked us to sing part of their Human Practices iGEM song, which would be used to create a iGEM song with all the participating teams. We submitted the first part of the song! The result should be visible on the Jamboree.

Mexico

TecCEM HS asked for our help since they had problems getting their ordered DNA through customs. The DNA sequence for ferritin was ordered at IDT and ligated into the pSB1C3 backbone which then was transformed into E. coli. Their construct has an IPTG inducible promoter which was tested by IPTG induction. The SDS-page gel has shown that the protein is expressed when adding IPTG. This confirms that the construct works.


Picture: Picture shows that after IPTG induction a thick band is visible of approximately 42kD