Difference between revisions of "Team:BostonU/Interlab"

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Revision as of 20:03, 17 September 2015

Our Project

Interlab Study

Our team participated in the interlab study this year. The interlab study is coordinated effort by iGEM to gather fluorescence data from three known genetic devices across different labs from around the world. This is an effort to normalize fluorescence data that may have been recorded under different conditions.

Our team used biobrick cloning methods to make the constructs each of which consisted of a GFP gene under a different constitutive promoter. The data was collected using FACS. The data was then processed by negating all fluorescence from cells that were outliers in size or complexity. Then, all cells that expressed a negative fluorescence value were disregarded.