Difference between revisions of "Team:UFMG Brazil/Results"

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<h2> Project Results</h2>
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<h3>Results</h3>
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<p> DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in <i>Leishmania</i> host (Figure 1).</p>
  
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<h5>What should this page contain?</h5>
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<img class="centered-image" src="https://static.igem.org/mediawiki/2015/b/b3/Ufmg-brazil-results00.png"/>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project </li>
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<li> Considerations for replicating the experiments </li>
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<h5> <b>Figure 1</b> - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.</h5>
  
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<p> The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. <i>E. coli</i> strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).</p>
  
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<h4> Project Achievements </h4>
 
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
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<li>A list of linked bullet points of the successful results during your project</li>
 
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
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<h4>Inspiration</h4>
 
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<p>See how other teams presented their results.</p>
 
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
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Revision as of 20:07, 17 September 2015




Project

Overview

Problem and
Solution

Chassis

Devices and
kill switch

Lab Work

Safety

Notebook

Protocols

Results

Modeling

Practices

Overview

Integrated Human
Practices

Public
Engagement

Synenergene

Overview

Application
Scenarios

Techno-moral

Team

Our Team

Attributions

Collaborations

Sponsors




Results

DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in Leishmania host (Figure 1).

Figure 1 - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.

The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. E. coli strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).