|
|
| Line 171: |
Line 171: |
| | <div id="child1" style="display:none"> | | <div id="child1" style="display:none"> |
| | <ul> | | <ul> |
| − | <li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-model.html#deliverymodel" style="font-weight:bold;font-family:幼圆;color:black">Delivery model</a></li> | + | <li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/NJU-China-model.html" style="font-weight:bold;font-family:幼圆;color:black">Delivery model</a></li> |
| − | <li style="line-height:250%;margin-left:10%"><a href="#" style="font-weight:bold;font-family:幼圆;color:black">RNAi model</a></li> | + | <li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/RNAi" style="font-weight:bold;font-family:幼圆;color:black">RNAi model</a></li> |
| | + | <li style="line-height:250%;margin-left:10%"><a href="https://2015.igem.org/Team:NJU-China/signaling" style="font-weight:bold;font-family:幼圆;color:black">Signaling</a></li> |
| | </ul> | | </ul> |
| | </div> | | </div> |
| Line 193: |
Line 194: |
| | </TD> | | </TD> |
| | <TD width="73%" bgColor=#FFFFFF style="vertical-align:top;padding-left:80px;padding-right:80px;padding-top:50px;padding-bottom:50px;word-wrap:break-word;"> | | <TD width="73%" bgColor=#FFFFFF style="vertical-align:top;padding-left:80px;padding-right:80px;padding-top:50px;padding-bottom:50px;word-wrap:break-word;"> |
| − | <h1> Collaboration </h1>
| |
| − |
| |
| − | Since firstly taking part in iGEM, NJU-China has the tradition of communicating with
| |
| − |
| |
| − | other teams in China. We do not only exchange our ideas and experiences with others, but
| |
| − |
| |
| − | also spread the knowledge of synthetic biology as well as iGEM Competition in China.
| |
| − |
| |
| − | This year we kept the tradition and took part in several activities.
| |
| − |
| |
| − | <B>
| |
| − | These activities strengthened our understanding of synthetic biology and deepened the
| |
| − |
| |
| − | friendship with other teams. We benefit each other in a mutual way.
| |
| − | </B>
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | <h2> Collaboration with SYSU-Software </h2>
| |
| − |
| |
| − | We cooperated with the Software team from Sun Yat-sen University to develop the
| |
| − |
| |
| − | conditioned place preference (CPP) mice image recognition software, and utilize the
| |
| − |
| |
| − | software into our CPP test to record the reflection of mice. The software is able to
| |
| − |
| |
| − | present the statistic results in the form of heat maps, reflecting the movement of mice
| |
| − |
| |
| − | directly and vividly.
| |
| − |
| |
| − | <B>
| |
| − | We thank our collaborator for receiving us with attention and for such strong
| |
| − |
| |
| − | contribution to our work.
| |
| − | </B>
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | In this experiment, we have seven mice. Each mouse is placed in a pair of interconnected
| |
| − |
| |
| − | cages, including a bright one and a dark one. Our experiment requires us to record the
| |
| − |
| |
| − | mice movement in order to accomplish the following goals: <br>
| |
| − | 1.To tell which cage the mice in, bright one or dark one <br>
| |
| − | 2.Record the mice coordinate precisely <br>
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | It’s hard for us to finish such job in tradition way. So we decided to seek some
| |
| − |
| |
| − | software to assist us. However, the software available to us can not meet our demands.
| |
| − |
| |
| − | It is really tricky. Fortunately, in iGEM China Community, we contact SYSU-Software, a
| |
| − |
| |
| − | well-known software team and ask them to produce a tool for us. Their tool really
| |
| − |
| |
| − | contributes a lot. With their tool analyzing the video we record, we can obtain the
| |
| − |
| |
| − | following results: <br>
| |
| − | 1.The data of the mice coordinate, 30 times per second <br>
| |
| − | 2.Based on the data, the tool can generate a Heat Maps, from which, we can know which
| |
| − |
| |
| − | cage the mice prefer to stay <br>
| |
| − |
| |
| − | <B> Once again, we would like to thank SYSU-Software. </B>
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | <h2> Collaboration with NUDT_China </h2>
| |
| − |
| |
| − | 1.NUDT_China helped our to perform nanoparticle tracking analysis (NTA) to have a more
| |
| − |
| |
| − | precise determination of the quantity and size of secreted exosomes. They did a
| |
| − |
| |
| − | supereminent experiment for us and we collected the perfect data we want. <br>
| |
| − | 2.We also helped NUDT_China to construct a TAL effector.
| |
| − | The recognition sequence of TALE1 (provided by NUDT_CHINA): 5’-GGAGGCACCGGTGG -3’
| |
| − |
| |
| − | (14bp) <br>
| |
| − | The corresponding RVD: NN NN NI NN NN HD NI HD HD NN NN NG NN NN <br>
| |
| − |
| |
| − | <img src = ""> <br><br> <!-- 插图一 -->
| |
| − |
| |
| − | Figure 1 Sequence of TALE1 cDNA and deduced amino acids.
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | The construction of this implemented with Golden Gate TALEN and TAL Effector Kit 2.0 by
| |
| − |
| |
| − | Addgene. <br>
| |
| − |
| |
| − | We then reedited the vector pTAL1 provided by the kit to add EcoRI, SpeI and PstI
| |
| − |
| |
| − | cutting cite to make the TALE we built using this vector can meet the RCF10 standard.
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | <h2> Conferences with NJAU_China </h2>
| |
| − | We held several project intercommunion meetings with Nanjing Agricultural University and
| |
| − |
| |
| − | exchanged our ideas and experiences. During the meetings, we provided some suggestions
| |
| − |
| |
| − | on their project. We also offered many plasmids to support their experiments
| |
| − |
| |
| − | efficiently.
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | <h2> Mentoring FAFU-CHINA </h2>
| |
| − |
| |
| − | We helped the iGEM team from Fujian Agriculture and Forestry University in their topic
| |
| − |
| |
| − | revision and provided them with some suggestions. We also provided a vigorous support
| |
| − |
| |
| − | for their follow-on experiments.
| |
| − |
| |
| − | <br><br>
| |
| − |
| |
| − | <h2> Offering Parts </h2>
| |
| − |
| |
| − | We communicated experiments and modeling with the iGEM team from Northeast Agricultural
| |
| − |
| |
| − | University, and also offered RVG-Lamp 2b parts to help them finish their project.
| |
| | | | |
| | </TD> | | </TD> |
