Difference between revisions of "Team:UFMG Brazil/Results"
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<h3>Results</h3> | <h3>Results</h3> | ||
| − | <p> DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in <i>Leishmania</i> host (Figure 1).</p> | + | <p> DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in <i>Leishmania</i> host (Figure 1).<br/></p> |
<img class="centered-image" src="https://static.igem.org/mediawiki/2015/b/b3/Ufmg-brazil-results00.png"/> | <img class="centered-image" src="https://static.igem.org/mediawiki/2015/b/b3/Ufmg-brazil-results00.png"/> | ||
| − | <h5> <b>Figure 1</b> - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.</h5> | + | <h5> <b>Figure 1</b> - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.</h5><br/><br/> |
| − | <p> The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. <i>E. coli</i> strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).</p> | + | <p> The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. <i>E. coli</i> strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).<br/></p> |
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Revision as of 20:34, 17 September 2015
Project
Lab Work
Modeling
Practices
Synenergene
Team
Results
DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in Leishmania host (Figure 1).
Figure 1 - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.
The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. E. coli strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).
