Difference between revisions of "Team:KU Leuven/Research/Methods"
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<dd>2. Take 1 mL of the sample that you would like to quantify. Measure the OD(600 nm) and centrifuge till the cells are down (max speed 15000 rpm, 10 min). If you want to include the amount of AHL inside the cell, you should lyse the cells in advance.</dd> | <dd>2. Take 1 mL of the sample that you would like to quantify. Measure the OD(600 nm) and centrifuge till the cells are down (max speed 15000 rpm, 10 min). If you want to include the amount of AHL inside the cell, you should lyse the cells in advance.</dd> | ||
<dd>3. Inoculate the <i>C. violaceum</i> CV026 to OD(600 nm) = 0.1 in air-lid culture tubes containing LB and LB supplemented with 1 mL supernatans of your sample at 27°C (150 rev/min agitation) for 24 h in a shaking incubator. </dd> | <dd>3. Inoculate the <i>C. violaceum</i> CV026 to OD(600 nm) = 0.1 in air-lid culture tubes containing LB and LB supplemented with 1 mL supernatans of your sample at 27°C (150 rev/min agitation) for 24 h in a shaking incubator. </dd> | ||
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− | + | <p><b>References</b></p> | |
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− | + | <dd>1. iGEM ETH Zurich (2013). Modeling part overview <a href=”https://docs.google.com/document/d/1ba0T9HpIxsk0XKRHQpCnRwGk6e2kFNkFglwZHPA-8Nc/edit”> [online] </a> </dd> | |
− | + | <dd>2. Choo, J.H., Rukayadi, Y. and Hwang, J.K. (2005).Inhibition of bacterial quorum sensing by vanilla extract. Letters in Applied Microbiology. <a href=”http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2006.01928.x/epdf”> [online] </a></dd> | |
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Revision as of 21:41, 17 September 2015
Methods
On this page you can find all of the methods and protocols used in the lab to obtain our results. For some techniques, we included some basic theory, since it is a prerequisite to get acquainted with the theory behind these techniques before using them. To learn more about them, click the titles below!
Contact
Address: Celestijnenlaan 200G room 00.08 - 3001 Heverlee
Telephone: +32(0)16 32 73 19
Email: igem@chem.kuleuven.be