Difference between revisions of "Team:Aix-Marseille/Measurement"

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<p class="space20"><div align="justify"><span style ="font-family:Courier New">We choose to use 4 different methods to study the strength of the different promoters:
 
<p class="space20"><div align="justify"><span style ="font-family:Courier New">We choose to use 4 different methods to study the strength of the different promoters:
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The results show that the promoter J23100 is the strongest in all methods. In contrary the promoter J23117 is weaker than the other promoters. The promoter T7 hasn’t got basal expression detectable.
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The promoter’s order of the strength is little bit variable depends on the different methods.
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<TABLE BORDER="5">
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  <TR>
 +
<TH></TH>
 +
<TH> TECAN  </TH>
 +
<TH>  Fluorometer  </TH>
 +
<TH>  Magicbox  </TH>
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<TH>  Confocal Microscopy</TH>
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  </TR>
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  <TR>
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<TH>J23100</TH>
 +
<TD>+++++</TD>
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<TD>+++++</TD>
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<TD>+++++</TD>
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<TD>+++++</TD>
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  </TR>
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<TR>
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<TH>J23101</TH>
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<TD>++++</TD>
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<TD>++++</TD>
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<TD>+++</TD>
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<TD>+++++</TD>
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  </TR>
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<TR>
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<TH>J23118</TH>
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<TD>++++</TD>
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<TD>+++</TD>
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<TD>+++++</TD>
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<TD>+++++</TD>
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  </TR>
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<TR>
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<TH>J23106</TH>
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<TD>++</TD>
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<TD>++</TD>
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<TD>+</TD>
 +
<TD>+</TD>
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  </TR>
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<TR>
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<TH>J23117</TH>
 +
<TD>+</TD>
 +
<TD>+</TD>
 +
<TD>+</TD>
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<TD>+</TD>
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  </TR>
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<TR>
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<TH>P T7</TH>
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<TD>-</TD>
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<TD>-</TD>
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<TD>-</TD>
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<TD>-</TD>
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  </TR>
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</TABLE>
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Revision as of 22:13, 17 September 2015

Chew fight

Interlab study results

Results

The Team AMU decided to study 5 different promoters (J23117, J23106, J23118, J23101 and J23100) and the promoter T7 that we used for ours construction. We want to know what the basal expression of the T7 promoter is.

Measurement methods

We choose to use 4 different methods to study the strength of the different promoters:
  • - TECAN
  • - Fluorometer
  • - Magicbox
  • - Confocal Microscopy
Each method was calibrated thanks to the molecule Atto488. Only the confocal microscopy couldn’t be calibrated.

TECAN results

Fluorometer results

Magicbox results

Confocal microscopy results

Conclusion

The results show that the promoter J23100 is the strongest in all methods. In contrary the promoter J23117 is weaker than the other promoters. The promoter T7 hasn’t got basal expression detectable. The promoter’s order of the strength is little bit variable depends on the different methods.

TECAN Fluorometer Magicbox Confocal Microscopy
J23100 +++++ +++++ +++++ +++++
J23101 ++++ ++++ +++ +++++
J23118 ++++ +++ +++++ +++++
J23106 ++ ++ + +
J23117 + + + +
P T7 - - - -

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  • Aix Marseille Université
  • Marseille
  • France

Chew figth project, for the iGEM competition. See you soon in Boston !