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Revision as of 22:49, 17 September 2015
Miniprep
Liquid culture (pSB1C3-Holin/Endolysin x4 and pSB1C3-pDawn x2)
Liquid culture from the bacterial growth kinetic experiment (pDawn-Holin/Endolysin x2)
Digestion
| Tube | Miniprep | pDawn-H/E |
|---|---|---|
| H2O MQ | 2 μL | 3 μL |
| Buffer 2.1 | 1 μL | 1 μL |
| DNA | 5 μL | 5 μL |
| EcoRI | 1 μL | 1 μL |
| PstI | 1 μL | - |
37°C, 1h
Expected results
Results
There were no expected profile of digestion excepted for the first and the third wells with pDawn-Holin/Endolysin.
Digestion
| Tube | Biobrick |
|---|---|
| H2O MQ | 5 μL |
| Buffer 2.1 | 1 μL |
| DNA | 3 μL |
| EcoRI | 1 μL |
37°C, 1h
Liquid culture of bacteria transformed with pSB1C3-nanoluc (x5)
16 Sept. 15