Difference between revisions of "Team:NJAU China/Parts"

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<h2> Part Documentation</h2>
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<body>
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<div class="decration">
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<img src="https://static.igem.org/mediawiki/2015/f/fc/NJAU_China2015_teamparts.jpg" />
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<div class="highlightBox">
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<h4>Note</h4>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<div class="main_content">
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<h2>BBa_K1649000</h2>
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<h4>Adding parts to the registry</h4>
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<p>red light sensor</p>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<div class="first_down"><span>T</span>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<p>he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.</p>
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</div>
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<img src="https://static.igem.org/mediawiki/2015/f/f0/NJAU_2015_teamparts1.png"/>
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<h4>What information do I need to start putting my parts on the Registry?</h4>
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<h2>BBa_K1649001</h2>
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<p>The information needed to initially create a part on the Registry is:</p>
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<p>red light sensor with a GFP reporter</p>
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<ul>
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<div class="first_down"><span class="first_down">G</span>
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<li>Part Name</li>
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<p>FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.</p>
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<li>Part type</li>
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</div>
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<li>Creator</li>
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<img src="https://static.igem.org/mediawiki/2015/6/64/NJAU_2015_teamparts2.png" />
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<h2>BBa_K1649002</h2>
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<li>Long Description (Longer description of what the DNA does)</li>
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<p>Red light sensor with TetR not gate and GFP reporter</p>
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<li>Design considerations</li>
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<div class="first_down"><span class="first_down">A</span>
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</ul>
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<p>s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.</p>
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</div>
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<p>
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<img src="https://static.igem.org/mediawiki/2015/1/11/NJAU_2015_teamparts3.png" />
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
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<h2>BBa_K1649004</h2>
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<p>YF1-FixJ with terminator</p>
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<div class="first_down"><span class="first_down">T</span>
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<p>he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.</p>
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</div>
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<img src="https://static.igem.org/mediawiki/2015/0/04/NJAU_2015_teamparts4.png" />
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<h4>Inspiration</h4>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<h2>BBa_K1649003</h2>
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<p>YF1 blue light sensor with GFP reporter</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<div class="first_down"><span class="first_down">G</span>
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<ul>
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<p>FP is used to report the function of blue light sensor.</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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</div>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<img src="https://static.igem.org/mediawiki/2015/9/9b/NJAU_2015_teamparts5.png" />
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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</ul>
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<div class="sub_bottom">
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<h4>Part Table </h4>
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<groupparts>iGEM015 Example</groupparts>
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Revision as of 23:35, 17 September 2015

BBa_K1649000

red light sensor

T

he chimaera Cph8 is the phytochrome Cph1 fusing with a cyanobacterial photoreceptor EnvZ which can engineer a red light-regulated transcription system in E. coli, with the phycocyanobilin-biosynthesis genes (ho1 and pcyA). The promoter OmpC is repressed by the function of red light and activated by dark.

BBa_K1649001

red light sensor with a GFP reporter

G

FP can be used to evaluate the efficiency of red light sensor when this part is regulated by different promoters by measuring the florescence.

BBa_K1649002

Red light sensor with TetR not gate and GFP reporter

A

s the transcription from the red light sensor is repressed by the function of red light and activated by dark, we use a not gate, repressor TetR to ensure the system is activated by red light. GFP can be used to test whether the circuit works.

BBa_K1649004

YF1-FixJ with terminator

T

he LOV photo-switch YF1 was created by fusing the light perception domain of YtvA to the FixL kinase domain. YtvA acts as a blue-light photoreceptor and carries a blue-light-sensitive flavin-binding LOV domain. The response regulator FixJ forms a two-component system together with FixL. The sensor is inactive in light and active in the dark.

BBa_K1649003

YF1 blue light sensor with GFP reporter

G

FP is used to report the function of blue light sensor.