Difference between revisions of "Team:CSU Fort Collins/Results"
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<a href='#product'>3 Trans-zeatin Production</a><br> | <a href='#product'>3 Trans-zeatin Production</a><br> | ||
<a href='#design'>4 Kill Switch</a><br> | <a href='#design'>4 Kill Switch</a><br> | ||
| − | <a href='# | + | <a href='#ref'>5 References</a> |
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</p> | </p> | ||
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<p id='product'><br> | <p id='product'><br> | ||
<h4>Trans-zeatin Production Results</h4> | <h4>Trans-zeatin Production Results</h4> | ||
| − | We ran | + | We ran multiple growth experiments to assess how much metabolic stress, if any, our construct introduced into our strain. From the results, we can conclude that the presence of the genes do not increase metabolic stress on the cell.<br><br> |
| + | |||
<center> | <center> | ||
<img src='https://static.igem.org/mediawiki/2015/0/03/CSU_tZGrowth24.png' style='width:700px'/><br> | <img src='https://static.igem.org/mediawiki/2015/0/03/CSU_tZGrowth24.png' style='width:700px'/><br> | ||
| − | <i>Figure 1</i>: Growth comparison between a control strain and our trans-zeatin strain in shake flasks over 24 hours.<br> | + | <i>Figure 1</i>: Growth comparison between a control strain and our trans-zeatin strain in shake flasks over 24 hours.<br><br> |
<img src='https://static.igem.org/mediawiki/2015/2/28/CSU_tZGrowth72.png' style='width:700px'/><br> | <img src='https://static.igem.org/mediawiki/2015/2/28/CSU_tZGrowth72.png' style='width:700px'/><br> | ||
| − | <i>Figure 2</i>: Growth comparison between a control strain and our trans-zeatin strain in shake flasks over 72 hours.<br> | + | <i>Figure 2</i>: Growth comparison between a control strain and our trans-zeatin strain in shake flasks over 72 hours.<br><br> |
<img src='https://static.igem.org/mediawiki/2015/5/5c/CSU_tZGrowthBR.png' style='width:700px'/><br> | <img src='https://static.igem.org/mediawiki/2015/5/5c/CSU_tZGrowthBR.png' style='width:700px'/><br> | ||
| − | <i>Figure | + | <i>Figure 3</i>: Growth comparison between a control strain and our trans-zeatin strain in bioreactors over 72 hours.<br><br> |
</center> | </center> | ||
| + | Throughout these experiments, we also collected suspension samples which we purified and ran on a reverse-phase HPLC column. Initially, we extracted using 80% methanol and syringe filtration. The HPLC results had too much background to be conclusive, so we developed a new method, based on extraction of zeatin from coconut water<a href="#1"><sup>[1]</sup></a>. The procedure included | ||
</p> | </p> | ||
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<p id='design'><br> | <p id='design'><br> | ||
<h4>Kill Switch Results</h4> | <h4>Kill Switch Results</h4> | ||
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</p> | </p> | ||
<p id='ref'><br> | <p id='ref'><br> | ||
<h4>References</h4> | <h4>References</h4> | ||
| + | <ol> | ||
| + | <li> | ||
| + | |||
</p> | </p> | ||
Revision as of 00:29, 18 September 2015
COLORADO STATE
UNIVERSITY iGEM
Project Results
Key Results
Key Achievements- Construction and submission of multiple team parts
- Proof of the composite lac promoter:fadD:fadL part function
- Characterization of KillerRed in E. coli for use as an induced lethality switch
Key Challenges
- Reworking breakdown construct ideas after fadD experiment
- Inconclusive results about trans-zeatin production
- Proper handling and experimental design for testing KillerRed
Breakdown Results
Trans-zeatin Production Results
We ran multiple growth experiments to assess how much metabolic stress, if any, our construct introduced into our strain. From the results, we can conclude that the presence of the genes do not increase metabolic stress on the cell.
Figure 1: Growth comparison between a control strain and our trans-zeatin strain in shake flasks over 24 hours.
Figure 2: Growth comparison between a control strain and our trans-zeatin strain in shake flasks over 72 hours.
Figure 3: Growth comparison between a control strain and our trans-zeatin strain in bioreactors over 72 hours.
Kill Switch Results