Difference between revisions of "Team:Liceo Eugenio Hostos/Experiments"

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<h2>Experiments &amp; Protocols</h2>
 
<h2>Experiments &amp; Protocols</h2>
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<br>
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<h3>Protocols<h3>
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<p><ul>Bacterial transformation:
 +
<li>remove the biobriks that come in kits plates.
 +
<li>Put 10 cl water HQ in the well and pump that blended leaving 10 ul of a red substance. Then put the substance in had ependorf.
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<li> It should then be left with chemo-competent bacteria.
 +
<br>
 +
<li> Introducing: 100 ul of bacteria.
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<br>
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<li>Add 250 ul only to bacteria.
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<br>
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<li>2 ul plasmido.
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<br>
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<li>10 cold 0 °.
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<br>
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<li>42° min 50 sec Thermo blocked.
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<br>
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<li> 2 min 0 °.
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<br>
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<li>10 min T ° ambiente.
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<br><b><li>LB+Antibiotico.
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<li>Lb+CHLOR.
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<li>LB+Bacteria.
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<li>LB+Biobrick CHLOR.
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<li>5ml.</b>
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<li>5 antibiotic to Biobrick.
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<li>LB CHLOR.
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<li>10 ul min T ° environment.
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<li>Place all the bacteria in a culture.
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</p>
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 +
 +
 +
 +
 +
 +
 +
 +
 +
  
 
<p>Describe the experiments, research and protocols you used in your iGEM project.</p>
 
<p>Describe the experiments, research and protocols you used in your iGEM project.</p>

Revision as of 00:50, 18 September 2015

Experiments & Protocols


Protocols

    Bacterial transformation:
  • remove the biobriks that come in kits plates.
  • Put 10 cl water HQ in the well and pump that blended leaving 10 ul of a red substance. Then put the substance in had ependorf.
  • It should then be left with chemo-competent bacteria.
  • Introducing: 100 ul of bacteria.
  • Add 250 ul only to bacteria.
  • 2 ul plasmido.
  • 10 cold 0 °.
  • 42° min 50 sec Thermo blocked.
  • 2 min 0 °.
  • 10 min T ° ambiente.
  • LB+Antibiotico.
  • Lb+CHLOR.
  • LB+Bacteria.
  • LB+Biobrick CHLOR.
  • 5ml.
  • 5 antibiotic to Biobrick.
  • LB CHLOR.
  • 10 ul min T ° environment.
  • Place all the bacteria in a culture.

    Describe the experiments, research and protocols you used in your iGEM project.

    What should this page contain?
    • Protocols
    • Experiments
    • Documentation of the development of your project

    Inspiration