Difference between revisions of "Team:Central Calgary"
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− | <div id="inspire" class="white_content"><img src="https://static.igem.org/mediawiki/2015/6/68/ScienceCenter.jpg" alt="ScienceCenter.jpg" style="width:35%; float:left; padding:10px;"> <a href = "javascript:void(0)" onclick = "document.getElementById('light').style.display='none';document.getElementById('fade').style.display='none' | + | <div id="inspire" class="white_content"><img src="https://static.igem.org/mediawiki/2015/6/68/ScienceCenter.jpg" alt="ScienceCenter.jpg" style="width:35%; float:left; padding:10px;"> <a href = "javascript:void(0)" onclick = "document.getElementById('light').style.display='none';document.getElementById('fade').style.display='none'"></a>A few of the team members presented synthetic biology to children at the Telus Spark science centre. They explained our project to the greater public, and received some stunning feedback from visitors of all ages. Surveys given show that the majority of the individuals of the greater public would be willing to use a bacterium to cure cancer,taking into account the the risks of mutation. The majority believe as well that this form of treatment is ethical for human use. </div> |
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Revision as of 01:14, 18 September 2015
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Home Page
Meet The Team
Jared
Jared was once a no life, that is, until he joined IGEM -- of which then, he became a no life that does science. If he isn't in the lab, then in all likelihood, he would be attempting to learn idioms, reading papers, doing math, memorizing the quadratic formula, and devouring ice cream.
Jessica
Jessica Skall is a grade 12 student at Central Memorial Highschool. She recently moved to Calgary from Toronto, and she felt that iGem would be a good place to make friends with common interests in science. Long team plans of hers include becoming an international ESL teacher. That is all
Francis
Lorem ipsum dolor sit amet, consectetur adipiscing elit. In vestibulum magna eget nulla blandit, at faucibus enim fermentum. Sed malesuada, metus in lobortis rhoncus, nisi orci tempus leo, in finibus justo ligula ac lectus. Sed eu pharetra est. Vestibulum tincidunt nisl ex, placerat placerat dui sagittis at. Suspendisse vel neque non quam porttitor pharetra. Vivamus ac mauris id enim auctor bibendum. Vestibulum vel augue in risus faucibus luctus at ac libero. Sed et faucibus orci, sit amet tincidunt lacus. Pellentesque mattis quis lacus at fringilla. Curabitur ipsum nibh, fermentum at metus et, venenatis ullamcorper lorem. Pellentesque congue sit amet enim eu bibendum. Pellentesque tincidunt consequat magna. Ut eleifend arcu vel massa laoreet, at rutrum lacus dictum. Sed ut lorem ornare, sodales sem quis, imperdiet risus. Integer vitae eros vel dui dapibus gravida nec vel dui. Fusce at nisl interdum, dictum nisi sit amet, aliquam elit. Maecenas efficitur purus nunc, faucibus feugiat nulla vulputate et. Quisque a porttitor velit, nec dictum neque. Integer pretium egestas arcu, nec tempus ligula pharetra fermentum. Nam dapibus leo nec dui iaculis hendrerit. Sed in enim egestas, placerat felis vitae, luctus diam. Praesent non lobortis diam. Mauris pretium maximus enim, et ullamcorper eros suscipit sed. Integer.
Natalie
Natalie is a grade twelve student in Central's Performing and Visual Arts program in the dramatic arts discipline. She has always had a passion for the arts, having done private voice lessons from ages 8-16 as well as being involved in several choirs including Central Memorial's concert choir. Natalie, although always very academic, became extremely fascinated with human biology throughout high school. She worked as a research assistant in the lab of Dr. Marie Fraser at the University of Calgary during summer of 2015, work in on a biomedical research project with future application in dislipodemia and cancer treatment. Her future aspirations are to study Life Sciences or Health Sciences and one day become a perfusionist.
Danielle
My name is Danielle and I am currently a grade 12 student at Central Memorial High School. At central, I am a part of the Performing Visual Arts program for dance. I do many genres of dance such as jazz, tap, ballet, lyrical, hip hop, and more. It takes up most of my free time. I am also a co-captain of the cheerleading team, and a member of the iGem team. Being a part of iGem is a great experience! In the future, I aspire to be a doctor, and this is why I am a member of the iGem team. IGem has broadened my knowledge and will potentially help me in fulfilling my career goals.
Alexia
Alexia Gendron is a simple 12th grader who spends her spare time perfecting recipes and playing piano. She enjoys reading, volunteering, good soup, Italian, and having insightful conversations. Although born in Calgary, Alberta, she was brought up in French, and attended a francophone school until her 10th grade. This has given her exposure to French-Canadian culture and taught her where to find it even if she finds herself a few provinces away from its origin. For five years she took karate and developed quite a fondness for martial arts. Despite having had to stop karate this year, she plans on starting to practice judo as soon as possible. After discovering Ronda Rousey, she found the very first person ever to move her so strongly, and she considers Rousey to be an inspiration. Right now, Alexia is focusing on the present, and is trying to enjoy her final year of high school before she graduates. Although she plans on attending university next year, she has yet to figure out exactly what she wants to study. She has always loved to learn all there is to learn and do all there is to do; she hopes to travel the world someday, meet fantastical people, and have a great collection of jaw-dropping stories to tell.
Zenab
Zenab Bhamgerwala is a grade 12 student at Central Memorial High School. She joined the iGEM team because of the vast amount of opportunities it offered. Through central iGEM, Zenab was able to perform various procedures in the lab and gain a great amount of experience in the world of synthetic biology. Outside of school she enjoys being the programming lead of her Robotics team, sketching, reading, and running long distance. In the future, Zenab hopes to pursue her passion of biomedical studies in post secondary. Joining this team has helped develop essential life skills and reaffirm her career decisions.
Vlad
Vladimir has been living in Calgary since day one. Growing up, he has experienced and explored everything from the bike paths in Fish Creek to the top of the Bow. At the age of eight, Vladimir developed a passion of photography and filmmaking, and carries it with him to this day. If he is not capturing photos, or studying, you can most likely find him zipping around from city to city on his bike. His passion for iGEM developed within the first weeks of grade ten, and carries into many aspects of his life. From lab work to media, Vladimir does it all.
Attributions
Thank you for you generous sponsorship
For giving us an opportunity and something worth my spare time
Special Thanks to: David Lloyd, our mentor
Iain George our other mentor
Ms.Wyshinski, who is not a biologist but kept us on track
Mr.Klemmer for his great support
The Project; The Dream 1
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The Project; The Dream 2
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec lacinia, mauris vel porta pellentesque, lectus mi aliquam erat, ut ultricies ex orci id massa. Cras ultrices lacinia libero sed porta. Pellentesque tempor vehicula leo, sit amet viverra dui posuere et. Vivamus gravida et felis ut interdum. Phasellus quis quam enim. Nullam suscipit viverra ex, sit amet bibendum massa scelerisque et. Nunc a lorem nisi. Integer ultricies metus sed mi pretium, vitae aliquet mi egestas. Duis gravida tellus quis sem scelerisque varius. Mauris erat lorem, congue sit amet erat eu, bibendum laoreet nisl. Morbi ac massa eu quam pulvinar lacinia lacinia et dui. Aliquam sed ex lobortis, lobortis tellus ut, pretium sem. Aenean id semper lacus. Sed sed mauris lobortis, tincidunt sapien vel, dictum ipsum. Nulla euismod dui at placerat pulvinar.
The Project; The Dream 3
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The Project; The Dream 4
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec lacinia, mauris vel porta pellentesque, lectus mi aliquam erat, ut ultricies ex orci id massa. Cras ultrices lacinia libero sed porta. Pellentesque tempor vehicula leo, sit amet viverra dui posuere et. Vivamus gravida et felis ut interdum. Phasellus quis quam enim. Nullam suscipit viverra ex, sit amet bibendum massa scelerisque et. Nunc a lorem nisi. Integer ultricies metus sed mi pretium, vitae aliquet mi egestas. Duis gravida tellus quis sem scelerisque varius. Mauris erat lorem, congue sit amet erat eu, bibendum laoreet nisl. Morbi ac massa eu quam pulvinar lacinia lacinia et dui. Aliquam sed ex lobortis, lobortis tellus ut, pretium sem. Aenean id semper lacus. Sed sed mauris lobortis, tincidunt sapien vel, dictum ipsum. Nulla euismod dui at placerat pulvinar.
The Project; The Dream 5
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec lacinia, mauris vel porta pellentesque, lectus mi aliquam erat, ut ultricies ex orci id massa. Cras ultrices lacinia libero sed porta. Pellentesque tempor vehicula leo, sit amet viverra dui posuere et. Vivamus gravida et felis ut interdum. Phasellus quis quam enim. Nullam suscipit viverra ex, sit amet bibendum massa scelerisque et. Nunc a lorem nisi. Integer ultricies metus sed mi pretium, vitae aliquet mi egestas. Duis gravida tellus quis sem scelerisque varius. Mauris erat lorem, congue sit amet erat eu, bibendum laoreet nisl. Morbi ac massa eu quam pulvinar lacinia lacinia et dui. Aliquam sed ex lobortis, lobortis tellus ut, pretium sem. Aenean id semper lacus. Sed sed mauris lobortis, tincidunt sapien vel, dictum ipsum. Nulla euismod dui at placerat pulvinar.
The Project; The Dream 6
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec lacinia, mauris vel porta pellentesque, lectus mi aliquam erat, ut ultricies ex orci id massa. Cras ultrices lacinia libero sed porta. Pellentesque tempor vehicula leo, sit amet viverra dui posuere et. Vivamus gravida et felis ut interdum. Phasellus quis quam enim. Nullam suscipit viverra ex, sit amet bibendum massa scelerisque et. Nunc a lorem nisi. Integer ultricies metus sed mi pretium, vitae aliquet mi egestas. Duis gravida tellus quis sem scelerisque varius. Mauris erat lorem, congue sit amet erat eu, bibendum laoreet nisl. Morbi ac massa eu quam pulvinar lacinia lacinia et dui. Aliquam sed ex lobortis, lobortis tellus ut, pretium sem. Aenean id semper lacus. Sed sed mauris lobortis, tincidunt sapien vel, dictum ipsum. Nulla euismod dui at placerat pulvinar.
The Project; The Dream 7
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec lacinia, mauris vel porta pellentesque, lectus mi aliquam erat, ut ultricies ex orci id massa. Cras ultrices lacinia libero sed porta. Pellentesque tempor vehicula leo, sit amet viverra dui posuere et. Vivamus gravida et felis ut interdum. Phasellus quis quam enim. Nullam suscipit viverra ex, sit amet bibendum massa scelerisque et. Nunc a lorem nisi. Integer ultricies metus sed mi pretium, vitae aliquet mi egestas. Duis gravida tellus quis sem scelerisque varius. Mauris erat lorem, congue sit amet erat eu, bibendum laoreet nisl. Morbi ac massa eu quam pulvinar lacinia lacinia et dui. Aliquam sed ex lobortis, lobortis tellus ut, pretium sem. Aenean id semper lacus. Sed sed mauris lobortis, tincidunt sapien vel, dictum ipsum. Nulla euismod dui at placerat pulvinar.
Lab Protocol 1: Making LB Broth
This protocol is used to make LB Broth. This like every protocol has been adapted from the Synthetic Biology Guidebook for iGEM High School. Important note for all protocols: Everything must be sterile, and kept as aseptic as possible, throughout all procedures.
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Lab Protocol 1.5: Making LB Plates
This protocol is used to make LB Plates. Note how this protocol makes 500ml of LB Agar for 20-25 plates. It can be scaled up and down as required.
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Lab Protocol 2: Using the DNA Plate - Rehydrating
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Lab Protocol 3: Making Competent Cells
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Lab Protocol 4: Restriction Digestion
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Lab Protocol 5: Ligation
A few notes about the ligation: There should be a 3:1, insert DNA (the segment being added) to the vector DNA (the now linear plasmid backbone). Also, the amount of DNA in the ligation should be low, around 100ng.
The DNA concentration is assumed to be the same for both the insert and vector restriction digests, and it equals 25ng/µl, as set up by the previous protocol. Assuming the insert is about half the size of the vector, to get a molar ratio of 3 insert : 1 Vector, the volume ratio must 1.5 insert :1 vector.
Ligation buffers are usually 10 fold, (10x) more concentrated than needed. Therefore, they need to be diluted 10 times, i.e. 1µl in a total volume in 10µl.
*A general formula for determining the volume ratio of insert to vector.*
Vi / Vv = 3 x (Cv / Ci) x (Mi / Mv)
Vi = Volume Insert
Vv = Volume Vector
Cv = DNA conc. of vector restriction digest
Ci = DNA conc. of insert restriction digest
Mi = Molecular weight i.e. approximate size of insert
Mv = Molecular weight i.e. approximate size of vector
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Lab Protocol 6: Transformation
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Lab Protocol: Miniprep
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Lab Protocol 8: Making 10X TAE Buffer
To prepare 10x TAE Buffer you must first prepare a solution of 0.5M EDTA (pH 8.0).
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Lab Protocol: Agarose Gel Electrophoresis
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Safety
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Ethics
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Logbook
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Outreach
Please click the images to see the explanation of the event